Invitrogen

CD8a Monoclonal Antibody (RPA-T8), PE, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

40 Published Figures

34 References

View all (532) CD8 antibodies

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CD8a Antibody (12-0088-80) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD8a PE and CD4 PerCP-Cy5.5. As expected based on known relative expression patterns, CD4 clone OKT4 (OKT-4) stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

Product Image Gallery

CD8a Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 APC, CD8a PE and CD4 PerCP-Cy5.5. As expected based on known relative expression patterns, CD4 clone OKT4 (OKT-4) stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue). {RE}

Product Details

12-0088-80

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 33 publications 33 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RPA-T8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657771

Product Specific Information

Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported: The RPA-T8 antibody been reported for use in flow cytometric analysis.

Applications Tested: This RPA-T8 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Increased levels of endogenous retroviruses trigger fibroinflammation and play a role in kidney disease development.

Nature communications

Dhillon P,Mulholland KA,Hu H,Park J,Sheng X,Abedini A,Liu H,Vassalotti A,Wu J,Susztak K

12-0088-80 was used in flow cytometry to indicate an important role of epigenetic derepression-induced ERV activation triggering renal fibroinflammation.

Thu Feb 02 00:00:00 EST 2023

Endothelial dysfunction markers and immune response indices in cosmonauts' blood after long-duration space flights.

NPJ microgravity

Kuzichkin DS,Nichiporuk IA,Zhuravleva OA,Markin AA,Rykova MP,Zhuravleva TV,Sadova AA,Kutko OV,Shmarov VA,Ponomarev SA

12-0088-80 was used in Flow cytometry/Cell sorting to investigat the markers of endothelial activation and damage (plasma concentrations of soluble thrombomodulin fraction (sTM), von Willebrand factor (vWF), highly sensitive C-reactive protein (hs-CRP)), as well as the level of D-dimer and compared them to the immunological parameters characterizing the state of human humoral and cellular immunity.

Wed Nov 02 00:00:00 EDT 2022

Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes.

Nature communications

Renner K,Schwittay T,Chaabane S,Gottschling J,Müller C,Tiefenböck C,Salewski JN,Winter F,Buchtler S,Balam S,Malfertheiner MV,Lubnow M,Lunz D,Graf B,Hitzenbichler F,Hanses F,Poeck H,Kreutz M,Orsó E,Burkhardt R,Niedermair T,Brochhausen C,Gessner A,Salzberger B,Mack M

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Fri May 21 00:00:00 EDT 2021

Multipeptide stimulated PBMCs generate T_EM/T_CM for adoptive cell therapy in multiple myeloma.

Oncotarget

Vardam-Kaur T,Pathangey LB,McCormick DJ,Bergsagel PL,Cohen PA,Gendler SJ

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Tue Sep 28 00:00:00 EDT 2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.

Theranostics

Zhong L,Shi W,Gan L,Liu X,Huo Y,Wu P,Zhang Z,Wu T,Peng H,Huang Y,Zhao Y,Yuan Y,Deng Z,Tang H

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

CD19 Chimeric Antigen Receptor-Exosome Targets CD19 Positive B-lineage Acute Lymphocytic Leukemia and Induces Cytotoxicity.

Cancers

Haque S,Vaiselbuh SR

12-0088-80 was used in Flow Cytometry to show that the novel CD19 CAR exosomes target the CD19 surface antigens of leukemic B-cells and can induce contact-dependent cytotoxicity.

Fri Mar 19 00:00:00 EDT 2021

Preventing ATP Degradation by ASO-Mediated Knockdown of CD39 and CD73 Results in A2aR-Independent Rescue of T Cell Proliferation.

Molecular therapy. Nucleic acids

Festag J,Thelemann T,Schell M,Raith S,Michel S,Jaschinski F,Klar R

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Fri Sep 04 00:00:00 EDT 2020

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Molecular therapy oncolytics

Hammill JA,Kwiecien JM,Dvorkin-Gheva A,Lau VWC,Baker C,Wu Y,Bezverbnaya K,Aarts C,Heslen CW,Denisova GF,Derocher H,Milne K,Nelson BH,Bramson JL

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models.

Stem cell reports

Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Tue Nov 14 00:00:00 EST 2017

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.

Stem cells international

Chen J,Wang Q,Feng X,Zhang Z,Geng L,Xu T,Wang D,Sun L

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Ly J,Lagman M,Saing T,Singh MK,Tudela EV,Morris D,Anderson J,Daliva J,Ochoa C,Patel N,Pearce D,Venketaraman V

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.

PloS one

Lagman M,Ly J,Saing T,Kaur Singh M,Vera Tudela E,Morris D,Chi PT,Ochoa C,Sathananthan A,Venketaraman V

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Wed Dec 30 00:00:00 EST 2015

Immune response to JC virus T antigen in patients with and without colorectal neoplasia.

Gut microbes

Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients.

Central-European journal of immunology

Biylgi O,Karagöz B,Türken O,Gültepe M,Özgün A,Tunçel T,Emirzeoğlu L,Çelik S,Müftüoğlu T,Gökhan Kandemir E

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Thu Jul 09 00:00:00 EDT 2015

Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.

Oncotarget

Ye SB,Li ZL,Luo DH,Huang BJ,Chen YS,Zhang XS,Cui J,Zeng YX,Li J

12-0088 was used in Flow cytometry/Cell sorting to show that tumour-derived exosomes promote tumour progression and T-cell dysfunction through regulation of exosomal microRNAs in human carcinomas.

Wed Jul 30 00:00:00 EDT 2014

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

A mutation in the human Uncoordinated 119 gene impairs TCR signaling and is associated with CD4 lymphopenia.

Blood

Gorska MM,Alam R

12-0088 was used in Flow cytometry/Cell sorting to show that the V22G mutation of Unc119 represents a novel genetic defect in ICL.

Thu Feb 09 00:00:00 EST 2012

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

Frequency of circulating regulatory T cells increases during chronic HIV infection and is largely controlled by highly active antiretroviral therapy.

PloS one

Presicce P,Orsborn K,King E,Pratt J,Fichtenbaum CJ,Chougnet CA

12-0088 was used in Flow cytometry/Cell sorting to analyse Treg frequency and phenotype in 11 HIV-infected individuals enrolled in a single, prospective clinical trial.

Fri Jul 20 00:00:00 EDT 2012

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2009

B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.

Retrovirology

Brudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

Published figure using CD8a monoclonal antibody (Product # 12-0088-80) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2009

Expression and function of PDCD1 at the human maternal-fetal interface.

Biology of reproduction

Taglauer ES,Trikhacheva AS,Slusser JG,Petroff MG

12-0088 was used in Flow cytometry/Cell sorting to examine whether human maternal T cells express PDCD1.

Mon Sep 01 00:00:00 EDT 2008

The proapoptotic factors Bax and Bak regulate T Cell proliferation through control of endoplasmic reticulum Ca(2+) homeostasis.

Immunity

Jones RG,Bui T,White C,Madesh M,Krawczyk CM,Lindsten T,Hawkins BJ,Kubek S,Frauwirth KA,Wang YL,Conway SJ,Roderick HL,Bootman MD,Shen H,Foskett JK,Thompson CB

12-0088 was used in Flow cytometry/Cell sorting to establish the role of Bax and Bak in viable T cell function.

Wed Aug 01 00:00:00 EDT 2007

CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers.

The Journal of biological chemistry

Pang DJ,Hayday AC,Bijlmakers MJ

12-0088 was used in Immunofluorescence to investigate raft localisation into CD8αβ heterodimers versus CD8αα homodimers in human cells.

Fri May 04 00:00:00 EDT 2007

CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers.

The Journal of biological chemistry

Pang DJ,Hayday AC,Bijlmakers MJ

12-0088 was used in Immunofluorescence to investigate raft localisation into CD8αβ heterodimers versus CD8αα homodimers in human cells.

Fri May 04 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

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CD8a Monoclonal Antibody (RPA-T8), PE, eBioscience™

CD8a Antibody (12-0088-80) in Flow

Product Details

12-0088-80

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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