Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine5, eBioscience™

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Antibody Testing Data (1)
Published Figures (15)

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 16

CD90.2 (Thy-1.2) Antibody (15-0902-82) in Flow

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.03 µg Anti-Mouse CD90-2 (Thy-1-2) PE-Cyanine5 (filled histogram).Total cells were used for analysis.

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Product Details

15-0902-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

53-2.1

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468783

Product Specific Information

Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: The 53-2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

A single-cell transcriptional atlas reveals resident progenitor cell niche functions in TMJ disc development and injury.

Nature communications

Bi R,Yin Q,Li H,Yang X,Wang Y,Li Q,Fang H,Li P,Lyu P,Fan Y,Ying B,Zhu S

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2023

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

Extracellular HMGB-1 activates inflammatory signaling in tendon cells and tissues.

Therapeutic advances in chronic disease

Zhang C,Gu X,Zhao G,Wang W,Shao J,Zhu J,Yuan T,Sun J,Nie D,Zhou Y

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Sun Apr 17 00:00:00 EDT 2022

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter.

Nature communications

Yang R,Yu T,Kou X,Gao X,Chen C,Liu D,Zhou Y,Shi S

15-0902 was used in Flow cytometry/Cell sorting to indicate that Tet1 and Tet2 play a role in maintaining mesenchymal stem cell and bone homeostasis through demethylation of P2rX7.

Fri Jun 01 00:00:00 EDT 2018

Mutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.

The Journal of clinical investigation

Zang S,Li J,Yang H,Zeng H,Han W,Zhang J,Lee M,Moczygemba M,Isgandarova S,Yang Y,Zhou Y,Rao A,You MJ,Sun D,Huang Y

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Cardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene.

Circulation research

Lombardi R,Chen SN,Ruggiero A,Gurha P,Czernuszewicz GZ,Willerson JT,Marian AJ

15090282 was used in flow cytometry to discuss the cellular origins of fibroadipocytes in arrhythmogenic cardiomyopathy

Fri Jun 24 00:00:00 EDT 2016

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Nature biotechnology

Kim J,Li WA,Choi Y,Lewin SA,Verbeke CS,Dranoff G,Mooney DJ

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

Nature communications

Singh NK,Kotla S,Dyukova E,Traylor JG,Orr AW,Chernoff J,Marion TN,Rao GN

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Wed Jun 24 00:00:00 EDT 2015

Enhancing T lineage production in aged mice: a novel function of Foxn1 in the bone marrow niche.

Journal of immunology (Baltimore, Md. : 1950)

Zook EC,Zhang S,Gerstein RM,Witte PL,Le PT

15-0902 was used in Flow cytometry/Cell sorting to report that Foxn1 is expressed in wild-type BM and overexpressed in Foxn1Tg.

Sun Dec 01 00:00:00 EST 2013

Partial characterization of the Sox2+ cell population in an adult murine model of digit amputation.

Tissue engineering. Part A

Agrawal V,Siu BF,Chao H,Hirschi KK,Raborn E,Johnson SA,Tottey S,Hurley KB,Medberry CJ,Badylak SF

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

Tracking epitope-specific T cells.

Nature protocols

Moon JJ,Chu HH,Hataye J,Pagán AJ,Pepper M,McLachlan JB,Zell T,Jenkins MK

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Tue Jun 30 00:00:00 EDT 2009

Manganese superoxide dismutase gene dosage affects chromosomal instability and tumor onset in a mouse model of T cell lymphoma.

Free radical biology & medicine

van de Wetering CI,Coleman MC,Spitz DR,Smith BJ,Knudson CM

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 15-0902-82) in Flow Cytometry

Tue Apr 15 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein

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Gene Aliases: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

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UniProt ID: (Mouse) P01831

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Entrez Gene ID: (Mouse) 21838

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Function(s)

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding cell adhesion molecule

Process(es)

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), PE-Cyanine5, eBioscience™

CD90.2 (Thy-1.2) Antibody (15-0902-82) in Flow

Product Details

15-0902-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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