Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor™ 450, eBioscience™

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 19

CD90.2 (Thy-1.2) Antibody (48-0902-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.015 µg of Rat IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.0075 µg of Anti-Mouse CD90.2 (Thy-1.2) eFluor® 450 (right). Total viable cells were used for analysis.

CD90.2 (Thy-1.2) Antibody in Immunocytochemistry (ICC/IF)

CD90.2 (Thy-1.2) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

48-0902-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.015 µg/test

View 28 publications 28 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

53-2.1

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272200

Product Specific Information

Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: This 53-2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A single-cell transcriptional atlas reveals resident progenitor cell niche functions in TMJ disc development and injury.

Nature communications

Bi R,Yin Q,Li H,Yang X,Wang Y,Li Q,Fang H,Li P,Lyu P,Fan Y,Ying B,Zhu S

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2023

CD8⁺ T cell-intrinsic IL-6 signaling promotes resistance to anti-PD-L1 immunotherapy.

Cell reports. Medicine

Huseni MA,Wang L,Klementowicz JE,Yuen K,Breart B,Orr C,Liu LF,Li Y,Gupta V,Li C,Rishipathak D,Peng J,Şenbabaoǧlu Y,Modrusan Z,Keerthivasan S,Madireddi S,Chen YJ,Fraser EJ,Leng N,Hamidi H,Koeppen H,Ziai J,Hashimoto K,Fassò M,Williams P,McDermott DF,Rosenberg JE,Powles T,Emens LA,Hegde PS,Mellman I,Turley SJ,Wilson MS,Mariathasan S,Molinero L,Merchant M,West NR

48-0902-82 was used in Flow cytometry/Cell sorting to identify interleukin 6 (IL-6) as a correlate of poor response to atezolizumab (anti-PD-L1) in large clinical trials of advanced kidney, breast, and bladder cancers.

Tue Jan 17 00:00:00 EST 2023

Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing.

Signal transduction and targeted therapy

Liu W,Chen W,Xie M,Chen C,Shao Z,Zhang Y,Zhao H,Song Q,Hu H,Xing X,Cai X,Deng X,Li X,Wang P,Liu G,Xiong L,Lv X,Zhang Y

48-0902-82 was used in Flow cytometry/Cell sorting to conclude that TBI accelerates bone formation during early stage of fracture healing process by shaping the anti-inflammation environment in the bone marrow.

Wed Jul 05 00:00:00 EDT 2023

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis.

Nature communications

Beckmann D,Römer-Hillmann A,Krause A,Hansen U,Wehmeyer C,Intemann J,de Gorter DJJ,Dankbar B,Hillen J,Heitzmann M,Begemann I,Galic M,Weinhage T,Foell D,Ai R,Kremerskothen J,Kiener HP,Müller S,Kamradt T,Schröder C,Leitão E,Horsthemke B,Rosenstiel P,Nordström K,Gasparoni G,Gasparoni N,Walter J,Li N,Yang X,Chung HR,Pavenstädt H,Lindemann N,Schnittler HJ,Wang W,Firestein GS,Pap T,Korb-Pap A

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Immunohistochemistry

Tue Jun 15 00:00:00 EDT 2021

Astrocytes promote a protective immune response to brain Toxoplasma gondii infection via IL-33-ST2 signaling.

PLoS pathogens

Still KM,Batista SJ,O'Brien CA,Oyesola OO,Früh SP,Webb LM,Smirnov I,Kovacs MA,Cowan MN,Hayes NW,Thompson JA,Tait Wojno ED,Harris TH

48-0902 was used in Flow cytometry/Cell sorting to show that IL-33 is expressed by oligodendrocytes and astrocytes during T. gondii infection, is released locally into the cerebrospinal fluid of T. gondii-infected animals, and is required for control of infection.

Thu Oct 01 00:00:00 EDT 2020

Extracellular HMGB-1 activates inflammatory signaling in tendon cells and tissues.

Therapeutic advances in chronic disease

Zhang C,Gu X,Zhao G,Wang W,Shao J,Zhu J,Yuan T,Sun J,Nie D,Zhou Y

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Sun Apr 17 00:00:00 EDT 2022

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

IFN-γ promoted exosomes from mesenchymal stem cells to attenuate colitis via miR-125a and miR-125b.

Cell death & disease

Yang R,Huang H,Cui S,Zhou Y,Zhang T,Zhou Y

48-0902-82 was used in Flow Cytometry to investigate the potential anti-inflammatory effects of bone marrow mesenchymal stem cell-derived exosomes.

Thu Jul 30 00:00:00 EDT 2020

Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge.

Nature communications

Yi S,Zhai J,Niu R,Zhu G,Wang M,Liu J,Huang H,Wang Y,Jing X,Kang L,Song W,Shi Y,Tang H

48-0902 was used in Flow cytometry/Cell sorting to suggest that different lung antigen-presenting cells modulate lung cDC1-mediated eosinophil recruitment dynamically.

Mon Sep 24 00:00:00 EDT 2018

Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency.

Cell stem cell

Hernandez C,Wang Z,Ramazanov B,Tang Y,Mehta S,Dambrot C,Lee YW,Tessema K,Kumar I,Astudillo M,Neubert TA,Guo S,Ivanova NB

48-0902-82 was used in Flow cytometry/Cell sorting to show that embryonic stem cell (ESC)-specific factors Dppa2 and Dppa4 play a key role in resetting the epigenome to a pluripotent state.

Thu Sep 06 00:00:00 EDT 2018

Single-cell transcriptomics reconstructs fate conversion from fibroblast to cardiomyocyte.

Nature

Liu Z,Wang L,Welch JD,Ma H,Zhou Y,Vaseghi HR,Yu S,Wall JB,Alimohamadi S,Zheng M,Yin C,Shen W,Prins JF,Liu J,Qian L

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Immunofluorescence

Thu Nov 02 00:00:00 EDT 2017

Mutations in 5-methylcytosine oxidase TET2 and RhoA cooperatively disrupt T cell homeostasis.

The Journal of clinical investigation

Zang S,Li J,Yang H,Zeng H,Han W,Zhang J,Lee M,Moczygemba M,Isgandarova S,Yang Y,Zhou Y,Rao A,You MJ,Sun D,Huang Y

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Bacillus anthracis lethal toxin negatively modulates ILC3 function through perturbation of IL-23-mediated MAPK signaling.

PLoS pathogens

Seshadri S,Allan DSJ,Carlyle JR,Zenewicz LA

48-0902 was used in Flow cytometry/Cell sorting to study the effects of MAPK signalling pathways in group 3 innate immune lymphocytes using Bacillus anthracis.

Sun Oct 01 00:00:00 EDT 2017

Cross-Presentation of Soluble and Cell-Associated Antigen by Murine Hepatocytes Is Enhanced by Collectrin Expression.

Journal of immunology (Baltimore, Md. : 1950)

Dolina JS,Cechova S,Rudy CK,Sung SJ,Tang WW,Lee J,Hahn YS,Le TH

48-0902 was used in Flow cytometry/Cell sorting to identify the functional role of collectrin in the liver.

Wed Mar 15 00:00:00 EDT 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

DUOX1 mediates persistent epithelial EGFR activation, mucous cell metaplasia, and airway remodeling during allergic asthma.

JCI insight

Habibovic A,Hristova M,Heppner DE,Danyal K,Ather JL,Janssen-Heininger YM,Irvin CG,Poynter ME,Lundblad LK,Dixon AE,Geiszt M,van der Vliet A

48-0902 was used in Flow cytometry/Cell sorting to study and compare the levels of expression of EGFR ligands and EGFR activation between asthmatic and non-asthmatic subjects.

Thu Nov 03 00:00:00 EDT 2016

A Serial shRNA Screen for Roadblocks to Reprogramming Identifies the Protein Modifier SUMO2.

Stem cell reports

Borkent M,Bennett BD,Lackford B,Bar-Nur O,Brumbaugh J,Wang L,Du Y,Fargo DC,Apostolou E,Cheloufi S,Maherali N,Elledge SJ,Hu G,Hochedlinger K

48-0902 was used in Flow cytometry/Cell sorting to identify potent repressors of somatic cell reprogramming into induced pluripotent stem cells.

Tue May 10 00:00:00 EDT 2016

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Nature biotechnology

Kim J,Li WA,Choi Y,Lewin SA,Verbeke CS,Dranoff G,Mooney DJ

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Early virus-host interactions dictate the course of a persistent infection.

PLoS pathogens

Sullivan BM,Teijaro JR,de la Torre JC,Oldstone MB

48-0902 was used in Flow cytometry/Cell sorting to investigate the very early host-virus interactions that determine the establishment of viral persistence.

Thu Jan 01 00:00:00 EST 2015

Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.

Nature communications

Singh NK,Kotla S,Dyukova E,Traylor JG,Orr AW,Chernoff J,Marion TN,Rao GN

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Wed Jun 24 00:00:00 EDT 2015

CX₃CR1⁺ mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22.

The Journal of experimental medicine

Longman RS,Diehl GE,Victorio DA,Huh JR,Galan C,Miraldi ER,Swaminath A,Bonneau R,Scherl EJ,Littman DR

48-0902 was used in Flow cytometry/Cell sorting to study the role of CX3CR1+ mononuclear phagocytes in the development of colitis and disease progression following Citrobacter rodentium infection.

Mon Jul 28 00:00:00 EDT 2014

Lipidoid Nanoparticles Containing PD-L1 siRNA Delivered In Vivo Enter Kupffer Cells and Enhance NK and CD8(+) T Cell-mediated Hepatic Antiviral Immunity.

Molecular therapy. Nucleic acids

Dolina JS,Sung SS,Novobrantseva TI,Nguyen TM,Hahn YS

48-0902 was used in Flow cytometry/Cell sorting to demonstrate that PD-L1 knockdown on KCs is central in determining the outcome of liver viral infections, and they represent a new class of gene therapy.

Tue Feb 19 00:00:00 EST 2013

Airway CD8(+) T cells induced by pulmonary DNA immunization mediate protective anti-viral immunity.

Mucosal immunology

Bivas-Benita M,Gillard GO,Bar L,White KA,Webby RJ,Hovav AH,Letvin NL

48-0902 was used in Flow cytometry/Cell sorting to assess the protectiveness of pulmonary immunity induced by plasmid DNA formulated with the polymer polyethyleneimine.

Tue Jan 01 00:00:00 EST 2013

Intrarectal immunization and IgA antibody-secreting cell homing to the small intestine.

Journal of immunology (Baltimore, Md. : 1950)

Agnello D,Denimal D,Lavaux A,Blondeau-Germe L,Lu B,Gerard NP,Gerard C,Pothier P

48-0902 was used in Magnetic cell separation to investigate the organ distribution and the homing molecule expression of IgA Ab-secreting cells induced by intrarectal immunisation with a particulate Ag.

Wed May 01 00:00:00 EDT 2013

Partial characterization of the Sox2+ cell population in an adult murine model of digit amputation.

Tissue engineering. Part A

Agrawal V,Siu BF,Chao H,Hirschi KK,Raborn E,Johnson SA,Tottey S,Hurley KB,Medberry CJ,Badylak SF

48-0902 was used in Flow cytometry/Cell sorting to characterise the population of cells accumulating at the site of injury after treatment with ECM degradation products in a well-established model of murine digit amputation.

Sun Jul 01 00:00:00 EDT 2012

Partial characterization of the Sox2+ cell population in an adult murine model of digit amputation.

Tissue engineering. Part A

Agrawal V,Siu BF,Chao H,Hirschi KK,Raborn E,Johnson SA,Tottey S,Hurley KB,Medberry CJ,Badylak SF

Sun Jul 01 00:00:00 EDT 2012

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.

Blood

Schuman J,Chen Y,Podd A,Yu M,Liu HH,Wen R,Chen ZJ,Wang D

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2009

Tracking epitope-specific T cells.

Nature protocols

Moon JJ,Chu HH,Hataye J,Pagán AJ,Pepper M,McLachlan JB,Zell T,Jenkins MK

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Tue Jun 30 00:00:00 EDT 2009

Manganese superoxide dismutase gene dosage affects chromosomal instability and tumor onset in a mouse model of T cell lymphoma.

Free radical biology & medicine

van de Wetering CI,Coleman MC,Spitz DR,Smith BJ,Knudson CM

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 48-0902-82) in Flow Cytometry

Tue Apr 15 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein

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Gene Aliases: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

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UniProt ID: (Mouse) P01831

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Entrez Gene ID: (Mouse) 21838

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Function(s)

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding cell adhesion molecule

Process(es)

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol

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CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor™ 450, eBioscience™

CD90.2 (Thy-1.2) Antibody (48-0902-82) in Flow

Product Details

48-0902-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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