Invitrogen

FceR1 alpha Monoclonal Antibody (MAR-1), Biotin, eBioscience™

19 Published Figures

32 References

View all (61) FceR1 alpha antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (19)

FceR1 alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 20

FceR1 alpha Antibody (13-5898-82) in Flow

Staining of MC/9 cell line with 0.06 µg of Armenian Hamster IgG Isotype Control Biotin (Product # 13-4888-81) (open histogram) or 0.06 µg of Anti-Mouse Fc epsilon Receptor I alpha (Fc epsilon R1) Biotin (filled histogram)followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

FceR1 alpha Antibody in Western Blot (WB)

FceR1 alpha Antibody in Western Blot, Immunoprecipitation (WB, IP)

FceR1 alpha Antibody in Immunocytochemistry (ICC/IF)

FceR1 alpha Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

FceR1 alpha Antibody in Immunohistochemistry (IHC)

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Product Details

13-5898-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 25 publications 25 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MAR-1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466783

Product Specific Information

Description: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha; is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported: The MAR-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FceR1 (Fc epsilonR1 alpha) is a subunit of the high affinity receptor of IgE. Fc epsilonR1 alpha is a tetrameric complex consisting of one alpha, one ß and two additional subunits. The latter two are required for signal transduction activity. The Fc epsilonR1 complex plays an important role in triggering allergic responses, and the immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine, which are responsible for allergy symptoms, are released. The IgE receptor couples allergen and mast cells to initiate the inflammatory responses that are characteristic of disorders such as hay fever and asthma. The release of histamine and proteases leads to the synthesis of prostaglandins and leukotrienes-potent effectors of the hypersensitivity response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ganglioside GM3 deficiency enhances mast cell sensitivity.

The FEBS journal

Komuro M,Mizugaki H,Nagane M,Morimoto M,Fukuyama T,Ogihara K,Naya Y,Yokomori E,Kaneshima K,Kawakami Y,Kamiie J,Shibata Y,Suzuki M,Shimizu T,Kawashima N,Okamoto M,Ikeda T,Yamashita T

13-5898-82 was used in Flow cytometry/Cell sorting to elucidate the role of ganglioside GM3 in mast cells and skin inflammation.

Fri Sep 01 00:00:00 EDT 2023

NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis.

Frontiers in immunology

Guo X,Xu X,Li T,Yu Q,Wang J,Chen Y,Ding S,Zhu L,Zou G,Zhang X

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Tue Jan 18 00:00:00 EST 2022

SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis.

The Journal of biological chemistry

Cardenas RA,Gonzalez R,Sanchez E,Ramos MA,Cardenas EI,Rodarte AI,Alcazar-Felix RJ,Isaza A,Burns AR,Heidelberger R,Adachi R

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Inflammatory Type 2 cDCs Acquire Features of cDC1s and Macrophages to Orchestrate Immunity to Respiratory Virus Infection.

Immunity

Bosteels C,Neyt K,Vanheerswynghels M,van Helden MJ,Sichien D,Debeuf N,De Prijck S,Bosteels V,Vandamme N,Martens L,Saeys Y,Louagie E,Lesage M,Williams DL,Tang SC,Mayer JU,Ronchese F,Scott CL,Hammad H,Guilliams M,Lambrecht BN

13-5898 was used in Flow cytometry/Cell sorting to show that monocyte-derived inflammatory DCs’’ are a mixture of monocyte-derived MCs that have little migratory and APC potential and bona fide pre-cDCderived, CD26-expressing inf-cDC2s that depend on Flt3L but not on GM-CSF.

Tue Jun 16 00:00:00 EDT 2020

Long-term ex vivo expansion of mouse hematopoietic stem cells.

Nature protocols

Wilkinson AC,Ishida R,Nakauchi H,Yamazaki S

13-5898 was used in Flow cytometry/Cell sorting to describe a simple platform for the expansion of functional mouse hematopoietic stem cells ex vivo for >1 month under fully defined albumin-free conditions.

Sat Feb 01 00:00:00 EST 2020

Mutant Huntingtin affects toll-like receptor 4 intracellular trafficking and cytokine production in mast cells.

Journal of neuroinflammation

Pérez-Rodríguez MJ,Ibarra-Sánchez A,Román-Figueroa A,Pérez-Severiano F,González-Espinosa C

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Fri Mar 27 00:00:00 EDT 2020

Tespa1 plays a role in the modulation of airway hyperreactivity through the IL-4/STAT6 pathway.

Journal of translational medicine

Yang R,Wang G,Li L,He H,Zheng M,Lu L,Wu S

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Mon Nov 23 00:00:00 EST 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Type I interferon limits mast cell-mediated anaphylaxis by controlling secretory granule homeostasis.

PLoS biology

Kobayashi T,Shimabukuro-Demoto S,Tsutsui H,Toyama-Sorimachi N

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

Na⁺-H⁺ exchanger 1 determines atherosclerotic lesion acidification and promotes atherogenesis.

Nature communications

Liu CL,Zhang X,Liu J,Wang Y,Sukhova GK,Wojtkiewicz GR,Liu T,Tang R,Achilefu S,Nahrendorf M,Libby P,Guo J,Zhang JY,Shi GP

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Western Blot

Wed Sep 04 00:00:00 EDT 2019

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

Positive and Negative Regulatory Roles of C-Terminal Src Kinase (CSK) in FcεRI-Mediated Mast Cell Activation, Independent of the Transmembrane Adaptor PAG/CSK-Binding Protein.

Frontiers in immunology

Potuckova L,Draberova L,Halova I,Paulenda T,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Mon Oct 07 00:00:00 EDT 2019

IL-23 induced in keratinocytes by endogenous TLR4 ligands polarizes dendritic cells to drive IL-22 responses to skin immunization.

The Journal of experimental medicine

Yoon J,Leyva-Castillo JM,Wang G,Galand C,Oyoshi MK,Kumar L,Hoff S,He R,Chervonsky A,Oppenheim JJ,Kuchroo VK,van den Brink MR,Malefyt Rde W,Tessier PA,Fuhlbrigge R,Rosenstiel P,Terhorst C,Murphy G,Geha RS

13-5898 was used in Flow cytometry/Cell sorting to elucidate the mechanisms behind DC-derived IL-23 in a model of skin inflammation.

Mon Sep 19 00:00:00 EDT 2016

Mast cell-derived neurotrophin 4 mediates allergen-induced airway hyperinnervation in early life.

Mucosal immunology

Patel KR,Aven L,Shao F,Krishnamoorthy N,Duvall MG,Levy BD,Ai X

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2016

New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling.

Molecular and cellular biology

Bambouskova M,Polakovicova I,Halova I,Goel G,Draberova L,Bugajev V,Doan A,Utekal P,Gardet A,Xavier RJ,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling.

Molecular and cellular biology

Bambouskova M,Polakovicova I,Halova I,Goel G,Draberova L,Bugajev V,Doan A,Utekal P,Gardet A,Xavier RJ,Draber P

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Isolation of Mature (Peritoneum-Derived) Mast Cells and Immature (Bone Marrow-Derived) Mast Cell Precursors from Mice.

PloS one

Meurer SK,Neß M,Weiskirchen S,Kim P,Tag CG,Kauffmann M,Huber M,Weiskirchen R

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Mon Jul 24 00:00:00 EDT 2017

Deficiency of FcϵR1 Increases Body Weight Gain but Improves Glucose Tolerance in Diet-Induced Obese Mice.

Endocrinology

Lee YJ,Liu C,Liao M,Sukhova GK,Shirakawa J,Abdennour M,Iamarene K,Andre S,Inouye K,Clement K,Kulkarni RN,Banks AS,Libby P,Shi GP

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Western Blot

Sun Nov 01 00:00:00 EDT 2015

Tie2 Signaling Enhances Mast Cell Progenitor Adhesion to Vascular Cell Adhesion Molecule-1 (VCAM-1) through α4β1 Integrin.

PloS one

Kanemaru K,Noguchi E,Tokunaga T,Nagai K,Hiroyama T,Nakamura Y,Tahara-Hanaoka S,Shibuya A

13-5898 was used in Flow cytometry/Cell sorting to suggest that Tie2 signaling induces α4β1 integrin activation on BM-MCps for adhesion to VCAM-1.

Sun Jun 26 00:00:00 EDT 2016

A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.

Nature medicine

Schönhuber N,Seidler B,Schuck K,Veltkamp C,Schachtler C,Zukowska M,Eser S,Feyerabend TB,Paul MC,Eser P,Klein S,Lowy AM,Banerjee R,Yang F,Lee CL,Moding EJ,Kirsch DG,Scheideler A,Alessi DR,Varela I,Bradley A,Kind A,Schnieke AE,Rodewald HR,Rad R,Schmid RM,Schneider G,Saur D

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Immunofluorescence

Sat Nov 01 00:00:00 EDT 2014

Signaling pathways activated by a protease allergen in basophils.

Proceedings of the National Academy of Sciences of the United States of America

Rosenstein RK,Bezbradica JS,Yu S,Medzhitov R

13-5898 was used in Flow cytometry/Cell sorting to study signaling pathways activated by a protease allergen in basophils.

Tue Nov 18 00:00:00 EST 2014

T cell-derived IL-4/IL-13 protects mice against fatal Schistosoma mansoni infection independently of basophils.

Journal of immunology (Baltimore, Md. : 1950)

Schwartz C,Oeser K,Prazeres da Costa C,Layland LE,Voehringer D

13-5898 was used in Flow cytometry/Cell sorting to determine the contribution of cytokines and basophils for protective immunity against S. mansoni egg-induced pathology.

Wed Oct 01 00:00:00 EDT 2014

Innate lymphoid type 2 cells sustain visceral adipose tissue eosinophils and alternatively activated macrophages.

The Journal of experimental medicine

Molofsky AB,Nussbaum JC,Liang HE,Van Dyken SJ,Cheng LE,Mohapatra A,Chawla A,Locksley RM

13-5898 was used in Flow cytometry/Cell sorting to study how ILC2s are resident in visceral adipose tissue and promote eosinophils and alternatively activated macrophages, implicated in metabolic homeostasis.

Mon Mar 11 00:00:00 EDT 2013

Thymic stromal lymphopoietin induces corticosteroid resistance in natural helper cells during airway inflammation.

Nature communications

Kabata H,Moro K,Fukunaga K,Suzuki Y,Miyata J,Masaki K,Betsuyaku T,Koyasu S,Asano K

13-5898 was used in Flow cytometry/Cell sorting to investigate the role of the IL-33/NH-cell axis in corticosteroid resistance.

Thu Jun 12 00:00:00 EDT 2014

Ionizing radiation-induced expression of INK4a/ARF in murine bone marrow-derived stromal cell populations interferes with bone marrow homeostasis.

Blood

Carbonneau CL,Despars G,Rojas-Sutterlin S,Fortin A,Le O,Hoang T,Beauséjour CM

13-5898 was used in Flow cytometry/Cell sorting to investigate the effect of ionizing radiation on murine bone marrow-derived stromal cell populations, and overall bone marrow homeostasis.

Thu Jan 19 00:00:00 EST 2012

A critical role of IL-33 in experimental allergic rhinitis.

The Journal of allergy and clinical immunology

Haenuki Y,Matsushita K,Futatsugi-Yumikura S,Ishii KJ,Kawagoe T,Imoto Y,Fujieda S,Yasuda M,Hisa Y,Akira S,Nakanishi K,Yoshimoto T

13-5898 was used in Flow cytometry/Cell sorting to demonstrate that ragweed pollen-driven endogenous IL-33 contributes to the development of experimental allergic rhinitis responses.

Sun Jul 01 00:00:00 EDT 2012

Inflammatory dendritic cells--not basophils--are necessary and sufficient for induction of Th2 immunity to inhaled house dust mite allergen.

The Journal of experimental medicine

Hammad H,Plantinga M,Deswarte K,Pouliot P,Willart MA,Kool M,Muskens F,Lambrecht BN

13-5898 was used in Flow cytometry/Cell sorting to study the pathway by which house dust mite inhalation induces Th2 immunity.

Mon Sep 27 00:00:00 EDT 2010

In vivo cytotoxicity of type I CD20 antibodies critically depends on Fc receptor ITAM signaling.

Cancer research

de Haij S,Jansen JH,Boross P,Beurskens FJ,Bakema JE,Bos DL,Martens A,Verbeek JS,Parren PW,van de Winkel JG,Leusen JH

13-5898 was used in Flow cytometry/Cell sorting to study the role of antibody-Fc receptor cross-linking of antibody-antigen immune complexes in the absence of gamma-chain signalling in mediating the action of therapeutic antibodies against cancer.

Thu Apr 15 00:00:00 EDT 2010

MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.

Nature immunology

Perrigoue JG,Saenz SA,Siracusa MC,Allenspach EJ,Taylor BC,Giacomin PR,Nair MG,Du Y,Zaph C,van Rooijen N,Comeau MR,Pearce EJ,Laufer TM,Artis D

Wed Jul 01 00:00:00 EDT 2009

IL-21R is essential for epicutaneous sensitization and allergic skin inflammation in humans and mice.

The Journal of clinical investigation

Jin H,Oyoshi MK,Le Y,Bianchi T,Koduru S,Mathias CB,Kumar L,Le Bras S,Young D,Collins M,Grusby MJ,Wenzel J,Bieber T,Boes M,Silberstein LE,Oettgen HC,Geha RS

Published figure using FceR1 alpha monoclonal antibody (Product # 13-5898-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2009

Administration of IL-33 induces airway hyperresponsiveness and goblet cell hyperplasia in the lungs in the absence of adaptive immune system.

International immunology

Kondo Y,Yoshimoto T,Yasuda K,Futatsugi-Yumikura S,Morimoto M,Hayashi N,Hoshino T,Fujimoto J,Nakanishi K

13-5898 was used in Flow cytometry/Cell sorting to compare the effects of IL-18 and IL-33 on bone marrow-derived mast cells and basophils as well as non-polarised and T(h)2-polarised CD4(+) T cells in vitro.

Sun Jun 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: fc epsilon r1; Fc-epsilon RI-alpha; FcERI; High affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; RP11-550P17.3

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Gene Aliases: Fce1a; fcepsilonri; Fcer1a; FcERI; Fcr-5

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UniProt ID: (Mouse) P20489

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Entrez Gene ID: (Mouse) 14125

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Function(s)

protein binding IgE receptor activity IgE binding immunoglobulin receptor superfamily

Process(es)

activation of MAPK activity positive regulation of type I hypersensitivity serotonin secretion signal transduction cell surface receptor signaling pathway activation of JUN kinase activity leukotriene biosynthetic process Fc-epsilon receptor signaling pathway positive regulation of mast cell degranulation positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of peptidyl-tyrosine phosphorylation positive regulation of calcium-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FceR1 alpha Monoclonal Antibody (MAR-1), Biotin, eBioscience™

FceR1 alpha Antibody (13-5898-82) in Flow

Product Details

13-5898-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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