Invitrogen

HLA-DR Monoclonal Antibody (LN3), eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

36 Published Figures

51 References

View all (121) HLA-DR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HLA-DR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 42

HLA-DR Antibody (14-9956-82) in IHC (P)

Immunohistochemical analysis of HLA-DR was performed using formalin-fixed paraffin-embedded human lymph node (Follicular grade lymphoma) tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 d... View More

HLA-DR Antibody in Flow Cytometry (Flow)

HLA-DR Antibody in Immunocytochemistry (ICC/IF)

HLA-DR Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

HLA-DR Antibody in Immunohistochemistry (IHC)

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Product Details

14-9956-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.125 µg/test

View 32 publications 32 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

LN3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468639

Product Specific Information

Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes.

Applications Reported: The LN3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. Immunohistochemistry can be performed using frozen and Bouin's, or formalin/paraffin, human tissues.

Applications Tested: The LN3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Brain macrophages acquire distinct transcriptomes in multiple sclerosis lesions and normal appearing white matter.

Acta neuropathologica communications

Miedema A,Gerrits E,Brouwer N,Jiang Q,Kracht L,Meijer M,Nutma E,Peferoen-Baert R,Pijnacker ATE,Wesseling EM,Wijering MHC,Gabius HJ,Amor S,Eggen BJL,Kooistra SM

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Immunohistochemistry (Frozen)

Fri Jan 28 00:00:00 EST 2022

The P522R protective variant of PLCG2 promotes the expression of antigen presentation genes by human microglia in an Alzheimer's disease mouse model.

Alzheimer's & dementia : the journal of the Alzheimer's Association

Claes C,England WE,Danhash EP,Kiani Shabestari S,Jairaman A,Chadarevian JP,Hasselmann J,Tsai AP,Coburn MA,Sanchez J,Lim TE,Hidalgo JLS,Tu C,Cahalan MD,Lamb BT,Landreth GE,Spitale RC,Blurton-Jones M,Davtyan H

14-9956-82 was used in Immunohistochemistry (Frozen) to provide the first evidence that the PLCG2-P522R variant increases the capacity of microglia to recruit T cells and present antigens, promoting a microglial transcriptional state that has recently been shown to be reduced in AD patient brains.

Sat Oct 01 00:00:00 EDT 2022

The spectrum of tuberculosis described as differential DNA methylation patterns in alveolar macrophages and alveolar T cells.

Clinical epigenetics

Pehrson I,Sayyab S,Das J,Idh N,Paues J,Méndez-Aranda M,Ugarte-Gil C,Lerm M

14-9956-82 was used in Flow cytometry/Cell sorting to suggest that M. tuberculosis induces epigenetic reprogramming in immune cells of the lung compartment, reflected as a specific DNA methylation pattern.

Sat Dec 17 00:00:00 EST 2022

Activated CD4⁺ T cells and CD14^hiCD16⁺ monocytes correlate with antibody response following influenza virus infection in humans.

Cell reports. Medicine

Wong SS,Oshansky CM,Guo XJ,Ralston J,Wood T,Reynolds GE,Seeds R,Jelley L,Waite B,Jeevan T,Zanin M,Widdowson MA,Huang QS,Thomas PG,Webby RJ

14-9956 was used in Flow cytometry/Cell sorting to demonstrate that seroconversion failure after infection is a definable immunological phenomenon, associated with quantifiable cellular markers that can be used to improve diagnostics, vaccine efficacy, and epidemiologic efforts.

Tue Apr 20 00:00:00 EDT 2021

Activated CD4⁺ T cells and CD14^hiCD16⁺ monocytes correlate with antibody response following influenza virus infection in humans.

Cell reports. Medicine

Wong SS,Oshansky CM,Guo XJ,Ralston J,Wood T,Reynolds GE,Seeds R,Jelley L,Waite B,Jeevan T,Zanin M,Widdowson MA,Huang QS,Thomas PG,Webby RJ

Tue Apr 20 00:00:00 EDT 2021

The role of irreversible electroporation in promoting M1 macrophage polarization via regulating the HMGB1-RAGE-MAPK axis in pancreatic cancer.

Oncoimmunology

He C,Sun S,Zhang Y,Xie F,Li S

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Immunocytochemistry

Thu Mar 11 00:00:00 EST 2021

The role of irreversible electroporation in promoting M1 macrophage polarization via regulating the HMGB1-RAGE-MAPK axis in pancreatic cancer.

Oncoimmunology

He C,Sun S,Zhang Y,Xie F,Li S

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Immunocytochemistry

Thu Mar 11 00:00:00 EST 2021

Dimethyl Fumarate, an Approved Multiple Sclerosis Treatment, Reduces Brain Oxidative Stress in SIV-Infected Rhesus Macaques: Potential Therapeutic Repurposing for HIV Neuroprotection.

Antioxidants (Basel, Switzerland)

Garcia-Mesa Y,Xu HN,Vance P,Gruenewald AL,Garza R,Midkiff C,Alvarez-Hernandez X,Irwin DJ,Gill AJ,Kolson DL

14-9956-82 was used immunohistochemistry (paraffin) investigate if Dimethyl fumarate administration to simian immunodeficiency virus (SIV)-infected rhesus macaques would induce antioxidant enzyme expression and reduce oxidative injury and inflammation throughout the brain.

Tue Mar 09 00:00:00 EST 2021

Dimethyl Fumarate, an Approved Multiple Sclerosis Treatment, Reduces Brain Oxidative Stress in SIV-Infected Rhesus Macaques: Potential Therapeutic Repurposing for HIV Neuroprotection.

Antioxidants (Basel, Switzerland)

Garcia-Mesa Y,Xu HN,Vance P,Gruenewald AL,Garza R,Midkiff C,Alvarez-Hernandez X,Irwin DJ,Gill AJ,Kolson DL

Tue Mar 09 00:00:00 EST 2021

Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model.

Stem cell research & therapy

Fan Y,Sun J,Zhang Q,Lai D

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Wed Mar 24 00:00:00 EDT 2021

Plaque-associated human microglia accumulate lipid droplets in a chimeric model of Alzheimer's disease.

Molecular neurodegeneration

Claes C,Danhash EP,Hasselmann J,Chadarevian JP,Shabestari SK,England WE,Lim TE,Hidalgo JLS,Spitale RC,Davtyan H,Blurton-Jones M

14-9956-82 was used in Immunohistochemistry (Frozen) to indicate lipid droplet accumulation occurs in human DAM xMGs in AD, but is reduced in TREM2-R47H DAM xMGs, as it occurs secondary to TREM2-mediated changes in plaque proximity and reactivity.

Fri Jul 23 00:00:00 EDT 2021

Lung transplantation for patients with severe COVID-19.

Science translational medicine

Bharat A,Querrey M,Markov NS,Kim S,Kurihara C,Garza-Castillon R,Manerikar A,Shilatifard A,Tomic R,Politanska Y,Abdala-Valencia H,Yeldandi AV,Lomasney JW,Misharin AV,Budinger GRS

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation.

PLoS pathogens

Oliveira AER,Pereira MCA,Belew AT,Ferreira LRP,Pereira LMN,Neves EGA,Nunes MDCP,Burleigh BA,Dutra WO,El-Sayed NM,Gazzinelli RT,Teixeira SMR

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Characterization of HLA-G Regulation and HLA Expression in Breast Cancer and Malignant Melanoma Cell Lines upon IFN-γ Stimulation and Inhibition of DNA Methylation.

International journal of molecular sciences

Jørgensen N,Sayed A,Jeppesen HB,Persson G,Weisdorf I,Funck T,Hviid TVF

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Wed Jun 17 00:00:00 EDT 2020

Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

Journal of translational medicine

Ni HH,Zhang L,Huang H,Dai SQ,Li J

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

New GMP manufacturing processes to obtain thermostable HIV-1 gp41 virosomes under solid forms for various mucosal vaccination routes.

NPJ vaccines

Amacker M,Smardon C,Mason L,Sorrell J,Jeffery K,Adler M,Bhoelan F,Belova O,Spengler M,Punnamoottil B,Schwaller M,Bonduelle O,Combadière B,Stegmann T,Naylor A,Johnson R,Wong D,Fleury S

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer's disease.

Nature communications

McQuade A,Kang YJ,Hasselmann J,Jairaman A,Sotelo A,Coburn M,Shabestari SK,Chadarevian JP,Fote G,Tu CH,Danhash E,Silva J,Martinez E,Cotman C,Prieto GA,Thompson LM,Steffan JS,Smith I,Davtyan H,Cahalan M,Cho H,Blurton-Jones M

14-9956-82 was used in Immunohistochemistry (Frozen) to tREM2 deletion reduces microglial survival, impairs phagocytosis of key substrates including APOE, and inhibits SDF-1α/CXCR4-mediated chemotaxis, culminating in an impaired response to beta-amyloid plaques in vivo.

Fri Oct 23 00:00:00 EDT 2020

Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer's disease.

Nature communications

Fri Oct 23 00:00:00 EDT 2020

Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer's disease.

Nature communications

Fri Oct 23 00:00:00 EDT 2020

Aquaporin-4 Expression during Toxic and Autoimmune Demyelination.

Cells

Rohr SO,Greiner T,Joost S,Amor S,Valk PV,Schmitz C,Kipp M

14-9956-82 was used in Immunohistochemistry (Paraffin) to provide strong evidence that the diffuse increase in anti-AQP4 staining intensity is due to a metabolic injury to the brain, whereas the focal, perivascular loss of anti-AQP4 immunoreactivity is mediated by peripheral immune cells.

Mon Sep 28 00:00:00 EDT 2020

Aquaporin-4 Expression during Toxic and Autoimmune Demyelination.

Cells

Rohr SO,Greiner T,Joost S,Amor S,Valk PV,Schmitz C,Kipp M

Mon Sep 28 00:00:00 EDT 2020

HspB5 Activates a Neuroprotective Glial Cell Response in Experimental Tauopathy.

Frontiers in neuroscience

Hampton DW,Amor S,Story D,Torvell M,Bsibsi M,van Noort JM,Chandran S

14-9956-82 was used in Immunohistochemistry (Frozen) to highlight the potentially broad therapeutic potential of HspB5 in neurodegenerative proteinopathies.

Wed Mar 23 00:00:00 EDT 2022

Targeting the mTOR pathway uncouples the efficacy and toxicity of PD-1 blockade in renal transplantation.

Nature communications

Esfahani K,Al-Aubodah TA,Thebault P,Lapointe R,Hudson M,Johnson NA,Baran D,Bhulaiga N,Takano T,Cailhier JF,Piccirillo CA,Miller WH

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Thu Oct 17 00:00:00 EDT 2019

Quantitative Interactomics in Primary T Cells Provides a Rationale for Concomitant PD-1 and BTLA Coinhibitor Blockade in Cancer Immunotherapy.

Cell reports

Celis-Gutierrez J,Blattmann P,Zhai Y,Jarmuzynski N,Ruminski K,Grégoire C,Ounoughene Y,Fiore F,Aebersold R,Roncagalli R,Gstaiger M,Malissen B

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses.

PLoS biology

Kaisar MMM,Ritter M,Del Fresno C,Jónasdóttir HS,van der Ham AJ,Pelgrom LR,Schramm G,Layland LE,Sancho D,Prazeres da Costa C,Giera M,Yazdanbakhsh M,Everts B

14-9956 was used in Flow cytometry/Cell sorting to study the molecular mechanisms through which dendritic cells prime T helper 2 responses.

Sun Apr 01 00:00:00 EDT 2018

AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells.

Oncology letters

Xiu W,Ma J,Lei T,Zhang M

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

Large-Scale Human Dendritic Cell Differentiation Revealing Notch-Dependent Lineage Bifurcation and Heterogeneity.

Cell reports

Balan S,Arnold-Schrauf C,Abbas A,Couespel N,Savoret J,Imperatore F,Villani AC,Vu Manh TP,Bhardwaj N,Dalod M

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Tue Aug 14 00:00:00 EDT 2018

Increased White Matter Inflammation in Aging- and Alzheimer's Disease Brain.

Frontiers in molecular neuroscience

Raj D,Yin Z,Breur M,Doorduin J,Holtman IR,Olah M,Mantingh-Otter IJ,Van Dam D,De Deyn PP,den Dunnen W,Eggen BJL,Amor S,Boddeke E

14-9956 was used in Immunohistochemistry to indicate that microglia-induced neuroinflammation is predominant in the white matter of aging mice and humans as well as in EOAD brains.

Thu Oct 01 00:00:00 EDT 2020

Plasma soluble CD163 is associated with postmortem brain pathology in human immunodeficiency virus infection.

AIDS (London, England)

Bryant AK,Moore DJ,Burdo TH,Lakritz JR,Gouaux B,Soontornniyomkij V,Achim CL,Masliah E,Grant I,Levine AJ,Ellis RJ

14-9956 was used in Immunohistochemistry to demonstrate that higher plasma sCD163 is associated with greater synaptodendritic damage and microglial activation in cortical and subcortical brain regions.

Mon Apr 24 00:00:00 EDT 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Gut-homing Δ42PD1⁺Vδ2 T cells promote innate mucosal damage via TLR4 during acute HIV type 1 infection.

Nature microbiology

Cheung AKL,Kwok HY,Huang Y,Chen M,Mo Y,Wu X,Lam KS,Kong HK,Lau TCK,Zhou J,Li J,Cheng L,Kiat Lee B,Peng Q,Lu X,An M,Wang H,Shang H,Zhou B,Wu H,Xu A,Yuen KY,Chen Z

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2017

FOXP1 suppresses immune response signatures and MHC class II expression in activated B-cell-like diffuse large B-cell lymphomas.

Leukemia

Brown PJ,Wong KK,Felce SL,Lyne L,Spearman H,Soilleux EJ,Pedersen LM,Møller MB,Green TM,Gascoyne DM,Banham AH

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Infection and immunity

Phuah J,Wong EA,Gideon HP,Maiello P,Coleman MT,Hendricks MR,Ruden R,Cirrincione LR,Chan J,Lin PL,Flynn JL

14-9956 was used in Flow cytometry/Cell sorting to examine the contribution of B cells and humoural immunity to the control of tuberculosis in non-human primates during the acute phase of infection.

Sun May 01 00:00:00 EDT 2016

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Pentraxin-3 regulates the inflammatory activity of macrophages.

Biochemistry and biophysics reports

Shiraki A,Kotooka N,Komoda H,Hirase T,Oyama JI,Node K

Published figure using HLA-DR monoclonal antibody (Product # 14-9956-82) in Immunocytochemistry

Tue Mar 01 00:00:00 EST 2016

The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function.

PloS one

van der Aa E,Buschow SI,Biesta PJ,Janssen HL,Woltman AM

14-9956 was used in Flow cytometry/Cell sorting to investigate the role of BDCA3+ DCs in immune response against the hepititis B virus, showing that BDCA3+ DCs are available in the blood and on site in HBV infected livers.

Wed Aug 02 00:00:00 EDT 2017

Local Immune Responses in Children and Adults with Allergic and Nonallergic Rhinitis.

PloS one

Lee KS,Yu J,Shim D,Choi H,Jang MY,Kim KR,Choi JH,Cho SH

14-9956 was used in Flow cytometry/Cell sorting to compare local immune responses between children and adults with AR and nonallergic rhinitis (NAR), and to investigate whether the association of local and systemic immune responses is different between the two age groups.

Thu Jul 27 00:00:00 EDT 2017

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-9956 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 EDT 2015

Higher Frequency of NK and CD4+ T-Cells in Mucosa and Potent Cytotoxic Response in HIV Controllers.

PloS one

Taborda NA,González SM,Alvarez CM,Correa LA,Montoya CJ,Rugeles MT

14-9956 was used in Flow cytometry/Cell sorting to investigate the frequency, phenotype and activity of T and NK cells in HIV controllers, showing higher frequencies of NK and CD4+ T-Cells and potent cytotoxic response.

Fri May 13 00:00:00 EDT 2016

An MHC II-dependent activation loop between adipose tissue macrophages and CD4+ T cells controls obesity-induced inflammation.

Cell reports

Cho KW,Morris DL,DelProposto JL,Geletka L,Zamarron B,Martinez-Santibanez G,Meyer KA,Singer K,O'Rourke RW,Lumeng CN

14-9956 was used in Immunofluorescence to demonstrate the importance of MHCII-restricted signals from adipose tissue macrophages that regulate adipose tissue T cell maturation and metainflammation.

Thu Oct 23 00:00:00 EDT 2014

Macrophages in inflammatory multiple sclerosis lesions have an intermediate activation status.

Journal of neuroinflammation

Vogel DY,Vereyken EJ,Glim JE,Heijnen PD,Moeton M,van der Valk P,Amor S,Teunissen CE,van Horssen J,Dijkstra CD

14-9956 was used in Immunohistochemistry to show that a major subset of macrophages have an intermediate activation status.

Mon Mar 04 00:00:00 EST 2013

PI3K-PKB hyperactivation augments human plasmacytoid dendritic cell development and function.

Blood

van de Laar L,van den Bosch A,Boonstra A,Binda RS,Buitenhuis M,Janssen HL,Coffer PJ,Woltman AM

14-9956 was used in Flow cytometry/Cell sorting to investigate the role of the PI3K-PKB-mTOR axis in human plasmacytoid dendritic cell development.

Thu Dec 13 00:00:00 EST 2012

Circulating myeloid-derived suppressor cells are increased and activated in pulmonary hypertension.

Chest

Yeager ME,Nguyen CM,Belchenko DD,Colvin KL,Takatsuki S,Ivy DD,Stenmark KR

14-9956 was used in Immunofluorescence to test whether immature myeloid cells would increased in the blood of patients with pulmonary hypertension compared with control subjects.

Sun Apr 01 00:00:00 EDT 2012

A role for interleukin-2 trans-presentation in dendritic cell-mediated T cell activation in humans, as revealed by daclizumab therapy.

Nature medicine

Wuest SC,Edwan JH,Martin JF,Han S,Perry JS,Cartagena CM,Matsuura E,Maric D,Waldmann TA,Bielekova B

14-9956-82 was used in Flow Cytometry to determine the participation of CD25 and interleukin-2 in the activation of T cells.

Sun May 01 00:00:00 EDT 2011

Small heat shock proteins induce a cerebral inflammatory reaction.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Bruinsma IB,de Jager M,Carrano A,Versleijen AA,Veerhuis R,Boelens W,Rozemuller AJ,de Waal RM,Verbeek MM

14-9956 was used in Immunohistochemistry to study the colocalisation of molecular chaperones Hsp20, HspB8 and HspB2B3 with cerebral amyloid angiopathy(CAA) and capillary CAA in Alzheimer's disease brains.

Wed Aug 17 00:00:00 EDT 2011

Comparison of gene expression profiles between human and mouse monocyte subsets.

Blood

Ingersoll MA,Spanbroek R,Lottaz C,Gautier EL,Frankenberger M,Hoffmann R,Lang R,Haniffa M,Collin M,Tacke F,Habenicht AJ,Ziegler-Heitbrock L,Randolph GJ

14-9956 was used in Flow cytometry/Cell sorting to identify how human and mouse monocyte subsets are far more broadly conserved than currently recognised.

Thu Jan 21 00:00:00 EST 2010

CD137 ligand signaling induces human monocyte to dendritic cell differentiation.

European journal of immunology

Kwajah M M S,Schwarz H

14-9956 was used in Flow cytometry/Cell sorting to deduce that peripheral monocytes can be differentiated into mature inflammatory dendritic cells by the single factor CD137.

Thu Jul 01 00:00:00 EDT 2010

Novel role of plasmacytoid dendritic cells in humans: induction of interleukin-10-producing Treg cells by plasmacytoid dendritic cells in patients with rheumatoid arthritis responding to therapy.

Arthritis and rheumatism

Kavousanaki M,Makrigiannakis A,Boumpas D,Verginis P

14-9956-82 was used in Flow Cytometry to explore the contribution of dendritic cells to the development of regulatory T cells in a human autoimmune disease setting.

Fri Jan 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DASS-397D15.1; DR beta-5; DR-9; DR2-beta-2; DR7; DR9; DRB1 transplantation antigen; Dw2; DW2.2/DR2.2; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLA-DRB1; HLA-DRB5; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; Human leukocyte antigen DRB1; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; leukocyte antigen; leukocyte antigen class II; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class I antigen; MHC Class II antigen; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen HLA-DR-beta; MHC class II HLA beta chain; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR beta 3 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC HLA DR-beta chain; MHC HLA-DR gamma chain; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain; p33; XXbac-BPG161M6.1

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Gene Aliases: CD74; DHLAG; DR-4; DR4; DRB1; DRB4; DRw10; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLADG; Ia-GAMMA; II; MLRW; SS1

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UniProt ID: (Human) P04233, (Human) P01903, (Human) Q5UBA2, (Human) P79483, (Human) P13762, (Human) Q30154

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Entrez Gene ID: (Human) 972, (Human) 3122, (Human) 3123, (Human) 3125, (Human) 3126, (Human) 3127

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Function(s)

beta-amyloid binding cytokine receptor activity protein binding cytokine binding MHC class II protein complex binding macrophage migration inhibitory factor binding MHC class II protein binding MHC class II protein binding, via antigen binding groove identical protein binding protein binding involved in protein folding nitric-oxide synthase binding MHC class II receptor activity peptide antigen binding major histocompatibility complex protein scaffold/adaptor protein

Process(es)

activation of MAPK activity prostaglandin biosynthetic process positive regulation of cytokine-mediated signaling pathway positive regulation of dendritic cell antigen processing and presentation negative regulation of peptide secretion positive regulation of type 2 immune response negative regulation of mature B cell apoptotic process protein complex assembly intracellular protein transport defense response signal transduction cell proliferation immunoglobulin mediated immune response antigen processing and presentation of endogenous antigen antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of B cell proliferation macrophage migration inhibitory factor signaling pathway regulation of macrophage activation negative regulation of apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator T cell selection positive thymic T cell selection negative thymic T cell selection negative regulation of T cell differentiation positive regulation of T cell differentiation positive regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration chaperone mediated protein folding requiring cofactor positive regulation of macrophage cytokine production positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production peptide antigen assembly with MHC class II protein complex antigen processing and presentation of peptide or polysaccharide antigen via MHC class II polysaccharide assembly with MHC class II protein complex immune response viral process T cell costimulation T cell receptor signaling pathway cognition interferon-gamma-mediated signaling pathway

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HLA-DR Monoclonal Antibody (LN3), eBioscience™

HLA-DR Antibody (14-9956-82) in IHC (P)

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14-9956-82

Western Blot (WB)

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