Invitrogen

IL-2 Monoclonal Antibody (MQ1-17H12), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

12 Published Figures

23 References

View all (144) IL-2 antibodies

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Datasheet

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FIGURE: 1 / 13

IL-2 Antibody (12-7029-81) in Flow

The Journal of experimental medicine 2018 - Figure 5. Rab6 and Syntaxin-16 are required for TCR-induced IL-2 production by T lymphocytes. (A-D) IL-2 production was measured in Jurkat cells activated for 6 h with Raji cells pulsed with different concentrations of SEE. (A and B) In cells expressing control or Rab6-specific shRNA, IL-2 in the supernatant (ELISA; A) or quantitative PCR of IL-2-specific cDNA (B). (C and D) In cells expressing control or S... View More

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IL-2 clone MQ1-17H12 stains a subset of CD4+ T cells only after stimulation (top) and does not stain CD19+ B cells regardless of stimulation (top and bottom). Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IL-2 (clone MQ1-17H12). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

12-7029-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ1-17H12

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466152

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported: MQ1-17H12 has been reported for use in intracellular flow cytometric analysis.

Applications Tested: This MQ1-17H12 antibody has been tested by intracellular staining of stimulated normal human perioheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T cell lymphoma treatment.

Molecular therapy oncolytics

Xia B,Lin K,Wang X,Chen F,Zhou M,Li Y,Lin Y,Qiao Y,Li R,Zhang W,He X,Zou F,Li L,Lu L,Chen C,Li W,Zhang H,Liu B

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2023

Dynamics of spike-and nucleocapsid specific immunity during long-term follow-up and vaccination of SARS-CoV-2 convalescents.

Nature communications

Koerber N,Priller A,Yazici S,Bauer T,Cheng CC,Mijočević H,Wintersteller H,Jeske S,Vogel E,Feuerherd M,Tinnefeld K,Winter C,Ruland J,Gerhard M,Haller B,Christa C,Zelger O,Roggendorf H,Halle M,Erber J,Lingor P,Keppler O,Zehn D,Protzer U,Knolle PA

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Mon Jan 10 00:00:00 EST 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.

Frontiers in cellular and infection microbiology

Kathamuthu GR,Kumar NP,Moideen K,Menon PA,Babu S

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Thu Nov 25 00:00:00 EST 2021

Platelets stimulate programmed death-ligand 1 expression by cancer cells: Inhibition by anti-platelet drugs.

Journal of thrombosis and haemostasis : JTH

Asgari A,Lesyk G,Poitras E,Govindasamy N,Terry K,To R,Back V,Rudzinski JK,Lewis JD,Jurasz P

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV.

Nature communications

Alrubayyi A,Gea-Mallorquí E,Touizer E,Hameiri-Bowen D,Kopycinski J,Charlton B,Fisher-Pearson N,Muir L,Rosa A,Roustan C,Earl C,Cherepanov P,Pellegrino P,Waters L,Burns F,Kinloch S,Dong T,Dorrell L,Rowland-Jones S,McCoy LE,Peppa D

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Tue Oct 05 00:00:00 EDT 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

CRISPR-Mediated Base Conversion Allows Discriminatory Depletion of Endogenous T Cell Receptors for Enhanced Synthetic Immunity.

Molecular therapy. Methods & clinical development

Preece R,Pavesi A,Gkazi SA,Stegmann KA,Georgiadis C,Tan ZM,Aw JYJ,Maini MK,Bertoletti A,Qasim W

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Fri Dec 11 00:00:00 EST 2020

A transcriptionally and functionally distinct PD-1⁺ CD8⁺ T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.

Nature medicine

Thommen DS,Koelzer VH,Herzig P,Roller A,Trefny M,Dimeloe S,Kiialainen A,Hanhart J,Schill C,Hess C,Savic Prince S,Wiese M,Lardinois D,Ho PC,Klein C,Karanikas V,Mertz KD,Schumacher TN,Zippelius A

12-7029 was used in Flow cytometry/Cell sorting to compare transcriptional, metabolic and functional signatures of intratumoral CD8+ T lymphocyte populations with high, intermediate and no PD-1 expression from non-small-cell lung cancer patients.

Sun Jul 01 00:00:00 EDT 2018

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.

Protein engineering, design & selection : PEDS

De Luca R,Kachel P,Kropivsek K,Snijder B,Manz MG,Neri D

Published figure using IL-2 monoclonal antibody (Product # 12-7029-81) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Activation-induced Markers Detect Vaccine-Specific CD4⁺ T Cell Responses Not Measured by Assays Conventionally Used in Clinical Trials.

Vaccines

Bowyer G,Rampling T,Powlson J,Morter R,Wright D,Hill AVS,Ewer KJ

12-7029 was used in Flow cytometry/Cell sorting to compare the use of markers for their sensitivity, specificity and associations with other measures of vaccine immunogenicity and demonstrate these as an additional method of reporting the T cell response to vaccination.

Tue Jul 31 00:00:00 EDT 2018

Safety and Immunogenicity of Malaria Vectored Vaccines Given with Routine Expanded Program on Immunization Vaccines in Gambian Infants and Neonates: A Randomized Controlled Trial.

Frontiers in immunology

Mensah VA,Roetynck S,Kanteh EK,Bowyer G,Ndaw A,Oko F,Bliss CM,Jagne YJ,Cortese R,Nicosia A,Roberts R,D'Alessio F,Leroy O,Faye B,Kampmann B,Cisse B,Bojang K,Gerry S,Viebig NK,Lawrie AM,Clarke E,Imoukhuede EB,Ewer KJ,Hill AVS,Afolabi MO

Mon Mar 14 00:00:00 EDT 2022

Long-lasting multifunctional CD8⁺ T cell responses in end-stage melanoma patients can be induced by dendritic cell vaccination.

Oncoimmunology

Wimmers F,Aarntzen EH,Duiveman-deBoer T,Figdor CG,Jacobs JF,Tel J,de Vries IJ

12-7029 was used in Flow cytometry/Cell sorting to investigate the functionality of tumour-specific CD8(+) T cells in melanoma patients upon dendritic cell vaccination, showing long-lasting multifunctional responses in end-stage patients.

Sat Jan 29 00:00:00 EST 2022

The human CD8+ T cell responses induced by a live attenuated tetravalent dengue vaccine are directed against highly conserved epitopes.

Journal of virology

Weiskopf D,Angelo MA,Bangs DJ,Sidney J,Paul S,Peters B,de Silva AD,Lindow JC,Diehl SA,Whitehead S,Durbin A,Kirkpatrick B,Sette A

12-7029 was used in Flow cytometry/Cell sorting to characterise the CD8(+) T cell responses after live attenuated dengue vaccination.

Thu Jan 01 00:00:00 EST 2015

Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-γ.

Journal of immunology (Baltimore, Md. : 1950)

Sundström P,Ahlmanner F,Akéus P,Sundquist M,Alsén S,Yrlid U,Börjesson L,Sjöling Å,Gustavsson B,Wong SB,Quiding-Järbrink M

12-7029 was used in Flow cytometry/Cell sorting to elucidate the presence and function of mucosa-associated invariant T cells in tumours.

Thu Oct 01 00:00:00 EDT 2015

Human γδ thymocytes are functionally immature and differentiate into cytotoxic type 1 effector T cells upon IL-2/IL-15 signaling.

Journal of immunology (Baltimore, Md. : 1950)

Ribot JC,Ribeiro ST,Correia DV,Sousa AE,Silva-Santos B

12-7029 was used in Flow cytometry/Cell sorting to study how ex vivo-isolated γδ thymocytes produced negligible IFN-γ and lacked cytolytic activity against leukemia cells.

Sat Mar 01 00:00:00 EST 2014

Hypomethylation at the regulatory T cell-specific demethylated region in CD25hi T cells is decoupled from FOXP3 expression at the inflamed site in childhood arthritis.

Journal of immunology (Baltimore, Md. : 1950)

Bending D,Pesenacker AM,Ursu S,Wu Q,Lom H,Thirugnanabalan B,Wedderburn LR

12-7029 was used in Flow cytometry/Cell sorting to study dissociation of CD25 and FOXP3 in CD4+ T cells at the site of inflammation in patients with juvenile arthritis..

Mon Sep 15 00:00:00 EDT 2014

Whole CMV proteome pattern recognition analysis after HSCT identifies unique epitope targets associated with the CMV status.

PloS one

Pérez-Bercoff L,Valentini D,Gaseitsiwe S,Mahdavifar S,Schutkowski M,Poiret T,Pérez-Bercoff Å,Ljungman P,Maeurer MJ

12-7029 was used in Flow cytometry/Cell sorting to screen the entire CMV proteome to visualise the humoral target epitope-focus profile in serum after haematopoietic stem cell transplantation.

Wed Jan 14 00:00:00 EST 2015

Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Weiskopf D,Angelo MA,de Azeredo EL,Sidney J,Greenbaum JA,Fernando AN,Broadwater A,Kolla RV,De Silva AD,de Silva AM,Mattia KA,Doranz BJ,Grey HM,Shresta S,Peters B,Sette A

12-7029 was used in Flow cytometry/Cell sorting to analyse dengue virus-specific responses, supporting an HLA-linked protective role for CD8+ T cells.

Tue May 28 00:00:00 EDT 2013

Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.

PLoS pathogens

Lindestam Arlehamn CS,Gerasimova A,Mele F,Henderson R,Swann J,Greenbaum JA,Kim Y,Sidney J,James EA,Taplitz R,McKinney DM,Kwok WW,Grey H,Sallusto F,Peters B,Sette A

12-7029 was used in Flow cytometry/Cell sorting to further define the immunological footprint of Mycobacterium tuberculosis CD4 T cell recognition.

Tue Jan 01 00:00:00 EST 2013

Evaluation of suppressive and pro-resolving effects of EPA and DHA in human primary monocytes and T-helper cells.

Journal of lipid research

Jaudszus A,Gruen M,Watzl B,Ness C,Roth A,Lochner A,Barz D,Gabriel H,Rothe M,Jahreis G

12-7029 was used in Flow cytometry/Cell sorting to demonstrate that EPA and DHA do not generally affect immune cell functions in an inhibitory manner.

Mon Apr 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: IL-2; IL2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)

Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-2 receptor binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

Process(es)

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IL-2 Monoclonal Antibody (MQ1-17H12), PE, eBioscience™

IL-2 Antibody (12-7029-81) in Flow

Product Details

12-7029-81

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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