Invitrogen

IL-2 Monoclonal Antibody (MQ1-17H12), APC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

21 References

View all (144) IL-2 antibodies

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FIGURE: 1 / 14

IL-2 Antibody (17-7029-41) in Flow

The Journal of experimental medicine 2018 - Figure 5. Rab6 and Syntaxin-16 are required for TCR-induced IL-2 production by T lymphocytes. (A-D) IL-2 production was measured in Jurkat cells activated for 6 h with Raji cells pulsed with different concentrations of SEE. (A and B) In cells expressing control or Rab6-specific shRNA, IL-2 in the supernatant (ELISA; A) or quantitative PCR of IL-2-specific cDNA (B). (C and D) In cells expressing control or S... View More

Product Image Gallery

IL-2 Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IL-2 clone MQ1-17H12 stains a subset of CD4+ T cells only after stimulation (top) and does not stain CD19+ B cells regardless of stimulation (top and bottom). Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IL-2 (clone MQ1-17H12). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

17-7029-41

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ1-17H12

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854572

Product Specific Information

Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.

Applications Reported: MQ1-17H12 has been reported for use in staining of intracellular IL-2.

Applications Tested: This MQ1-17H12 antibody is tested by intracellular staining and flow cytometric analysis of activated human normal human peripheral blood cells.This product has been pre-titrated and tested intracellular staining and flow cytometric analysis of activated human normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T cell lymphoma treatment.

Molecular therapy oncolytics

Xia B,Lin K,Wang X,Chen F,Zhou M,Li Y,Lin Y,Qiao Y,Li R,Zhang W,He X,Zou F,Li L,Lu L,Chen C,Li W,Zhang H,Liu B

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Thu Sep 21 00:00:00 EDT 2023

Dynamics of spike-and nucleocapsid specific immunity during long-term follow-up and vaccination of SARS-CoV-2 convalescents.

Nature communications

Koerber N,Priller A,Yazici S,Bauer T,Cheng CC,Mijočević H,Wintersteller H,Jeske S,Vogel E,Feuerherd M,Tinnefeld K,Winter C,Ruland J,Gerhard M,Haller B,Christa C,Zelger O,Roggendorf H,Halle M,Erber J,Lingor P,Keppler O,Zehn D,Protzer U,Knolle PA

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Mon Jan 10 00:00:00 EST 2022

Enhanced antibody responses in fully vaccinated individuals against pan-SARS-CoV-2 variants following Omicron breakthrough infection.

Cell reports. Medicine

Jeong HW,Kim SM,Jung MK,Noh JY,Yoo JS,Kim EH,Kim YI,Yu K,Jang SG,Gil J,Casel MA,Rare R,Choi JH,Kim HS,Kim JH,Um J,Kim C,Kim Y,Chin BS,Jung S,Choi JY,Song KH,Kim YD,Park JS,Song JY,Shin EC,Choi YK

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.

Frontiers in cellular and infection microbiology

Kathamuthu GR,Kumar NP,Moideen K,Menon PA,Babu S

17-7029-41 was used in Flow cytometry/Cell sorting to conclude that diminished γδ T cells expressing cytokine, cytotoxic, and other immune markers and elevated levels of cytokines in the supernatants is a characteristic feature of LTB PDM/DM co-morbidities.

Thu Nov 25 00:00:00 EST 2021

Decreased Frequencies of Gamma/Delta T Cells Expressing Th1/Th17 Cytokine, Cytotoxic, and Immune Markers in Latent Tuberculosis-Diabetes/Pre-Diabetes Comorbidity.

Frontiers in cellular and infection microbiology

Kathamuthu GR,Kumar NP,Moideen K,Menon PA,Babu S

Thu Nov 25 00:00:00 EST 2021

Platelets stimulate programmed death-ligand 1 expression by cancer cells: Inhibition by anti-platelet drugs.

Journal of thrombosis and haemostasis : JTH

Asgari A,Lesyk G,Poitras E,Govindasamy N,Terry K,To R,Back V,Rudzinski JK,Lewis JD,Jurasz P

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV.

Nature communications

Alrubayyi A,Gea-Mallorquí E,Touizer E,Hameiri-Bowen D,Kopycinski J,Charlton B,Fisher-Pearson N,Muir L,Rosa A,Roustan C,Earl C,Cherepanov P,Pellegrino P,Waters L,Burns F,Kinloch S,Dong T,Dorrell L,Rowland-Jones S,McCoy LE,Peppa D

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Tue Oct 05 00:00:00 EDT 2021

Rejuvenating Effector/Exhausted CAR T Cells to Stem Cell Memory-Like CAR T Cells By Resting Them in the Presence of CXCL12 and the NOTCH Ligand.

Cancer research communications

Ando M,Kondo T,Tomisato W,Ito M,Shichino S,Srirat T,Mise-Omata S,Nakagawara K,Yoshimura A

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

CRISPR-Mediated Base Conversion Allows Discriminatory Depletion of Endogenous T Cell Receptors for Enhanced Synthetic Immunity.

Molecular therapy. Methods & clinical development

Preece R,Pavesi A,Gkazi SA,Stegmann KA,Georgiadis C,Tan ZM,Aw JYJ,Maini MK,Bertoletti A,Qasim W

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Fri Dec 11 00:00:00 EST 2020

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors.

Nature communications

Webber BR,Lonetree CL,Kluesner MG,Johnson MJ,Pomeroy EJ,Diers MD,Lahr WS,Draper GM,Slipek NJ,Smeester BA,Lovendahl KN,McElroy AN,Gordon WR,Osborn MJ,Moriarity BS

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Tue Nov 19 00:00:00 EST 2019

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

A novel dual-cytokine-antibody fusion protein for the treatment of CD38-positive malignancies.

Protein engineering, design & selection : PEDS

De Luca R,Kachel P,Kropivsek K,Snijder B,Manz MG,Neri D

Published figure using IL-2 monoclonal antibody (Product # 17-7029-41) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients.

British journal of cancer

Wu X,Zhang H,Xing Q,Cui J,Li J,Li Y,Tan Y,Wang S

17-7029 was used in Immunofluorescence to evaluate the role of PD-1-PD-L1 pathway in CD8(+) T-cell functions in tumour-draining lymph nodes (TDLNs) and tumours of CRC patients.

Tue Sep 23 00:00:00 EDT 2014

PD-1(+) CD8(+) T cells are exhausted in tumours and functional in draining lymph nodes of colorectal cancer patients.

British journal of cancer

Wu X,Zhang H,Xing Q,Cui J,Li J,Li Y,Tan Y,Wang S

17-7029 was used in Immunofluorescence to evaluate the role of PD-1-PD-L1 pathway in CD8(+) T-cell functions in tumour-draining lymph nodes (TDLNs) and tumours of CRC patients.

Tue Sep 23 00:00:00 EDT 2014

Expansion of polyfunctional HIV-specific T cells upon stimulation with mRNA electroporated dendritic cells in the presence of immunomodulatory drugs.

Journal of virology

De Keersmaecker B,Allard SD,Lacor P,Schots R,Thielemans K,Aerts JL

17-7029 was used in Immunofluorescence to provide new information about the effects of IMiDs on antigen-specific T cells and suggest that these drugs increase the efficacy of immune therapies for infectious diseases and cancer.

Sat Sep 01 00:00:00 EDT 2012

Bet v 1-specific T-cell receptor/forkhead box protein 3 transgenic T cells suppress Bet v 1-specific T-cell effector function in an activation-dependent manner.

The Journal of allergy and clinical immunology

Schmetterer KG,Haiderer D,Leb-Reichl VM,Neunkirchner A,Jahn-Schmid B,Küng HJ,Schuch K,Steinberger P,Bohle B,Pickl WF

17-7029 was used in Flow cytometry/Cell sorting to demonstrate a transgenic approach to engineer customised, allergen-specific, human Treg cells, with potential implications for the treatment of allergic diseases.

Sat Jan 01 00:00:00 EST 2011

Longevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans.

PLoS pathogens

Teirlinck AC,McCall MB,Roestenberg M,Scholzen A,Woestenenk R,de Mast Q,van der Ven AJ,Hermsen CC,Luty AJ,Sauerwein RW

17-7029 was used in Flow cytometry/Cell sorting to study cellular immunological responses to sporozoites and asexual blood-stage malaria parasites.

Thu Dec 01 00:00:00 EST 2011

Human TCR transgenic Bet v 1-specific Th1 cells suppress the effector function of Bet v 1-specific Th2 cells.

Journal of immunology (Baltimore, Md. : 1950)

Neunkirchner A,Leb-Reichl VM,Schmetterer KG,Mutschlechner S,Kueng HJ,Haiderer D,Schuch K,Wallner M,Jahn-Schmid B,Bohle B,Pickl WF

17-7029 was used in Flow cytometry/Cell sorting to study the mechanisms underlying the cross-reactivity between the respiratory allergen, Bet v 1, and its homologous food allergens.

Sat Oct 15 00:00:00 EDT 2011

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

17-7029 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 EDT 2011

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

17-7029 was used in Flow cytometry/Cell sorting to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 EDT 2010

Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display.

Cancer research

Swoboda RK,Somasundaram R,Caputo L,Ochoa EM,Gimotty PA,Marincola FM,Van Belle P,Barth S,Elder D,Guerry D,Czerniecki B,Schuchter L,Vonderheide RH,Herlyn D

17-7029 was used in Flow cytometry/Cell sorting to investigate an antigen shared by melanoma and glioma cells that is recognised by T helper cells.

Sun Apr 15 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: IL-2; IL2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)

Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-2 receptor binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

Process(es)

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IL-2 Monoclonal Antibody (MQ1-17H12), APC, eBioscience™

IL-2 Antibody (17-7029-41) in Flow

Product Details

17-7029-41

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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