Invitrogen

IL-4 Monoclonal Antibody (11B11), Functional Grade, eBioscience™

FIGURE: 1 / 1

IL-4 Antibody (16-7041-81) in IS

Splenocytes from 8 week old mice pre-immunized with OVA/IFA were recall-activated with OVA antigen for 36 hours in Mouse IL-4 ELISPOT assay. Control well is medium alone.

Product Image Gallery

Product Details

16-7041-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 9 publications 9 publications

ELISA (ELISA)

View 5 publications 5 publications

Functional Assay (Functional)

Assay-Dependent

View 8 publications 8 publications

Inhibition Assays (IA)

View 1 publication 1 publication

T-Cell Activation (TCA)

View 3 publications 3 publications

In vitro Assay (IV)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Immunostaining (IS)

0.125 µg/mL

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

11B11

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469208

Product Specific Information

Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Reported: This 11B11 antibody has been reported for use in cytokine neutralization, ELISPOT, and ELISA cap.

Applications Tested: The Functional Grade Purified 11B11 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-4 bioactivity.

The 11B11 antibody at 0.125 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-4 in a CTLL-2 cell proliferation assay. Please refer to Best Protocols for more information.

The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-4 (IL-4) in combination with the biotinylated BVD6-24G2 (Product # 13-7042-85) antibody for detection and recombinant mouse IL-4 (Product # 14-8041-80) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.

Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

16-7041-81 was used in Flow Cytometry to study the role of ADAM12 in T-cell responses, fate determination on activation, and its functions in T cells to mediate tissue inflammation.

Sun Aug 01 00:00:00 EDT 2021

Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses.

Wellcome open research

Wu G,Murugesan G,Nagala M,McCraw A,Haslam SM,Dell A,Crocker PR

16-7041-81 was used in Cell Culture to investigate the molecular basis for Siglec-1 binding to regulatory T cells using in vitro-induced cells as a model system.

Tue Mar 01 00:00:00 EST 2022

Inhibition of Glycolysis in Pathogenic T_H17 Cells through Targeting a miR -21-Peli1-c-Rel Pathway Prevents Autoimmunity.

Journal of immunology (Baltimore, Md. : 1950)

Qiu R,Yu X,Wang L,Han Z,Yao C,Cui Y,Hou G,Dai D,Jin W,Shen N

16-7041-81 was used in Functional assay to investigate if CNS-infiltrated pathogenic TH17 cells from diseased mice specifically upregulate glycolytic pathway genes compared with homeostatic intestinal TH17 cells.

Mon Jun 15 00:00:00 EDT 2020

MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation.

Allergy, asthma & immunology research

Wang Z,Ji N,Chen Z,Sun Z,Wu C,Yu W,Hu F,Huang M,Zhang M

16-7041-95 was used in T-Cell Activation to investigate the associations between Th2 differentiation and miR-1165b-3p in asthma as well as the possible mechanisms.

Tue Sep 01 00:00:00 EDT 2020

The MicroRNA miR-22 Represses Th17 Cell Pathogenicity by Targeting PTEN-Regulated Pathways.

ImmunoHorizons

Wang L,Qiu R,Zhang Z,Han Z,Yao C,Hou G,Dai D,Jin W,Tang Y,Yu X,Shen N

16-7041-95 was used in In vitro Assay to identify that Th17 cell-intrinsic miR-22 could protect mice from autoimmunity by targeting PTEN-regulated pathways.

Tue Jun 09 00:00:00 EDT 2020

Runx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity.

Nature communications

Seo W,Shimizu K,Kojo S,Okeke A,Kohwi-Shigematsu T,Fujii SI,Taniuchi I

16-7041-81 was used in ELISA to suggest that RUNX3-mediated CCL5 repression is critical for modulating anti-tumor immunity.

Thu Mar 26 00:00:00 EDT 2020

Gasdermin D in peripheral myeloid cells drives neuroinflammation in experimental autoimmune encephalomyelitis.

The Journal of experimental medicine

Li S,Wu Y,Yang D,Wu C,Ma C,Liu X,Moynagh PN,Wang B,Hu G,Yang S

16-7041-81 was used in Flow Cytometry to further define the role of gasdermin D in experimental autoimmune encephalomyelitis.

Mon Nov 04 00:00:00 EST 2019

Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Deng Z,Gao X,Sun X,Amra S,Lu A,Cui Y,Eltzschig HK,Lei G,Huard J

16-7041 was used in Immunohistochemistry-immunofluorescence to investigate articular cartilage (AC) homeostasis and signaling pathways involved in the superior cartilage regeneration of Murphy Roths large (MRL/MpJ) mice.

Thu Aug 01 00:00:00 EDT 2019

Fetal T Cell Activation in the Amniotic Cavity during Preterm Labor: A Potential Mechanism for a Subset of Idiopathic Preterm Birth.

Journal of immunology (Baltimore, Md. : 1950)

Gomez-Lopez N,Romero R,Xu Y,Miller D,Arenas-Hernandez M,Garcia-Flores V,Panaitescu B,Galaz J,Hsu CD,Para R,Berry SM

16-7041-95 was used in T-Cell Activation to show that fetal T cells are a predominant leukocyte population in amniotic fluid during preterm gestrations, and that chord blood T cells obtained from women with idiopathic preterm labor and birth displayed enhanced ex vivo activation.

Tue Oct 01 00:00:00 EDT 2019

Proteomic Profiling of Mouse Helper T Cell Differentiation.

Proteomics

van den Ham HJ,Binai NA,Zaaraoui-Boutahar F,Heck AJR,Andeweg AC

16-7041-81 was used in In vitro Assay to support the design of prophylactic and therapeutic intervention strategies for (infectious) diseases.

Mon Apr 01 00:00:00 EDT 2019

The Sympathetic Nervous System Mitigates CNS Autoimmunity via β2-Adrenergic Receptor Signaling in Immune Cells.

Cell reports

Araujo LP,Maricato JT,Guereschi MG,Takenaka MC,Nascimento VM,de Melo FM,Quintana FJ,Brum PC,Basso AS

16-7041-81 was used in Flow Cytometry to uncover a suppressive role for the SNS in CNS autoimmunity while they identify potential targets for therapeutic intervention.

Tue Sep 17 00:00:00 EDT 2019

Distinct Regulation of Th17 and Th1 Cell Differentiation by Glutaminase-Dependent Metabolism.

Cell

Johnson MO,Wolf MM,Madden MZ,Andrejeva G,Sugiura A,Contreras DC,Maseda D,Liberti MV,Paz K,Kishton RJ,Johnson ME,de Cubas AA,Wu P,Li G,Zhang Y,Newcomb DC,Wells AD,Restifo NP,Rathmell WK,Locasale JW,Davila ML,Blazar BR,Rathmell JC

16-7041-81 was used in Functional assays to identify a key role for glutaminase (GLS) in T cell activation and specification.

Thu Dec 13 00:00:00 EST 2018

MicroRNA-210 overexpression promotes psoriasis-like inflammation by inducing Th1 and Th17 cell differentiation.

The Journal of clinical investigation

Wu R,Zeng J,Yuan J,Deng X,Huang Y,Chen L,Zhang P,Feng H,Liu Z,Wang Z,Gao X,Wu H,Wang H,Su Y,Zhao M,Lu Q

16-7041-81 was used in In vitro experiments to study the role of microRNA-210 in the immune imbalance of T lymphocyte subsets in psoriasis and suggest a potential therapeutic avenue.

Fri Jun 01 00:00:00 EDT 2018

Cytochrome c Oxidase Activity Is a Metabolic Checkpoint that Regulates Cell Fate Decisions During T Cell Activation and Differentiation.

Cell metabolism

Tarasenko TN,Pacheco SE,Koenig MK,Gomez-Rodriguez J,Kapnick SM,Diaz F,Zerfas PM,Barca E,Sudderth J,DeBerardinis RJ,Covian R,Balaban RS,DiMauro S,McGuire PJ

16-7041-81 was used in Flow cytometry/Cell sorting to understand the mechanism of cytochrome c oxidase regulation of OXPHOS using a mouse model with isolated dysfunction in T cells by targeting the COX10 gene which produces mitochondrial disease in humans.

Tue Jun 06 00:00:00 EDT 2017

Paeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation.

Scientific reports

Zhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H

16-7041 was used in Flow cytometry/Cell sorting to investigate the effect Paeoniflorin has on Th cells and dendritic cells in a mouse MS model.

Mon Feb 06 00:00:00 EST 2017

The RNA-binding protein HuR contributes to neuroinflammation by promoting C-C chemokine receptor 6 (CCR6) expression on Th17 cells.

The Journal of biological chemistry

Chen J,Martindale JL,Cramer C,Gorospe M,Atasoy U,Drew PD,Yu S

16-7041 was used in T-Cell Activation experiments to study how HuR contributes to T helper 17 cell-mediated autoimmune neuroinflammation, and its potential therapeutic target for managing autoimmune conditions of the central nervous system.

Fri Sep 01 00:00:00 EDT 2017

Metabolic control of T_H17 and induced T_reg cell balance by an epigenetic mechanism.

Nature

Xu T,Stewart KM,Wang X,Liu K,Xie M,Ryu JK,Li K,Ma T,Wang H,Ni L,Zhu S,Cao N,Zhu D,Zhang Y,Akassoglou K,Dong C,Driggers EM,Ding S

16-7041-81 was used in Immunohistochemistry, ELISA, Functional Assay to investigate T helper 17 cell differentiation and fate.

Thu Aug 10 00:00:00 EDT 2017

IL-15-dependent balance between Foxp3 and RORγt expression impacts inflammatory bowel disease.

Nature communications

Tosiek MJ,Fiette L,El Daker S,Eberl G,Freitas AA

16-7041 was used in an ELISA assay to demonstrate that impaired delivery of IL-15 to CD4+ T cells in the colon down-modulates Foxp3 expression and enhances RORγt expression.

Fri Mar 11 00:00:00 EST 2016

NADPH Oxidases Are Essential for Macrophage Differentiation.

The Journal of biological chemistry

Xu Q,Choksi S,Qu J,Jang J,Choe M,Banfi B,Engelhardt JF,Liu ZG

16-7041 was used in Functional assays to investigate the role of NOX1 and NOX2 in macrophage differentiation and tumor progression.

Fri Sep 16 00:00:00 EDT 2016

Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease.

PLoS neglected tropical diseases

Tsou YL,Lin YW,Shao HY,Yu SL,Wu SR,Lin HY,Liu CC,Huang C,Chong P,Chow YH

16-7041 was used in an ELISpot assay to develop a recombinant adenovirus Ad-EVVLP for the treatment of hand, foot and mouth disease, providing a possible vaccine for future immunisation against the disease.

Wed Apr 01 00:00:00 EDT 2015

Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis.

The Journal of allergy and clinical immunology

Nakashima C,Otsuka A,Kitoh A,Honda T,Egawa G,Nakajima S,Nakamizo S,Arita M,Kubo M,Miyachi Y,Kabashima K

16-7041 was used in Inhibition experiments to clarify the roles of eosinophils in irritant contact dermatitis and the underlying mechanism of eosinophil infiltration of the skin.

Tue Jul 01 00:00:00 EDT 2014

In vitro generated Th17 cells support the expansion and phenotypic stability of CD4(+)Foxp3(+) regulatory T cells in vivo.

Cytokine

Zhou Q,Hu Y,Howard OM,Oppenheim JJ,Chen X

16-7041 was used in Functional assays to demonstrate that the TNF-TNFR2 pathway plays a crucial role in the reciprocal stimulatory effect of Th17 cells and Tregs.

Wed Jan 01 00:00:00 EST 2014

Single-cell RNA sequencing reveals T helper cells synthesizing steroids de novo to contribute to immune homeostasis.

Cell reports

Mahata B,Zhang X,Kolodziejczyk AA,Proserpio V,Haim-Vilmovsky L,Taylor AE,Hebenstreit D,Dingler FA,Moignard V,Göttgens B,Arlt W,McKenzie AN,Teichmann SA

16-7041 was used in Functional assays to demonstrate the production of the steroid pregnenolone by Th2 cells in vitro and in vivo in a helminth infection model.

Thu May 22 00:00:00 EDT 2014

Staphylococcus aureus phenol-soluble modulin peptides modulate dendritic cell functions and increase in vitro priming of regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Schreiner J,Kretschmer D,Klenk J,Otto M,Bühring HJ,Stevanovic S,Wang JM,Beer-Hammer S,Peschel A,Autenrieth SE

16-7041 was used in Functional assays to demonstrate that PSMs from highly virulent pathogens influence dendritic cell functions, thereby modulating the adaptive immune response.

Mon Apr 01 00:00:00 EDT 2013

Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage.

Blood

Hendricks DW,Fink PJ

16-7041 was used in Flow cytometry/Cell sorting to investigate the relationships between recent thymic emigrants and T-helper type 1 and 2 cells.

Thu Jan 27 00:00:00 EST 2011

Soluble flagellin, FliC, induces an Ag-specific Th2 response, yet promotes T-bet-regulated Th1 clearance of Salmonella typhimurium infection.

European journal of immunology

Bobat S,Flores-Langarica A,Hitchcock J,Marshall JL,Kingsley RA,Goodall M,Gil-Cruz C,Serre K,Leyton DL,Letran SE,Gaspal F,Chester R,Chamberlain JL,Dougan G,López-Macías C,Henderson IR,Alexander J,MacLennan IC,Cunningham AF

16-7041 was used in an ELISPOT assay to show that, in mice, immunisation with soluble, recombinant FliC protein flagellin induces Th2 responses.

Wed Jun 01 00:00:00 EDT 2011

The metalloprotease-disintegrin ADAM8 is essential for the development of experimental asthma.

American journal of respiratory and critical care medicine

Naus S,Blanchet MR,Gossens K,Zaph C,Bartsch JW,McNagny KM,Ziltener HJ

16-7041 was used in Flow cytometry/Cell sorting to determine that ADAM8 plays a crucial proinflammatory role in airway inflammation.

Tue Jun 15 00:00:00 EDT 2010

Potent anti-tumor responses to immunization with dendritic cells loaded with tumor tissue and an NKT cell ligand.

Immunology and cell biology

Petersen TR,Sika-Paotonu D,Knight DA,Dickgreber N,Farrand KJ,Ronchese F,Hermans IF

16-7041 was used in Flow cytometry/Cell sorting to investigate the impact of eliciting T-cell help from activated iNKT cells using alpha-galactosylceramide, showing potent anti-tumour response.

Thu Jul 01 00:00:00 EDT 2010

Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.

Journal of immunology (Baltimore, Md. : 1950)

Weber KS,Hildner K,Murphy KM,Allen PM

16-7041 was used in Functional assays to study the regulation of unique Th cell subset calcium signals and their correlation to biological functions, showing that Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.

Wed Sep 01 00:00:00 EDT 2010

Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.

Journal of immunology (Baltimore, Md. : 1950)

Weber KS,Hildner K,Murphy KM,Allen PM

Wed Sep 01 00:00:00 EDT 2010

Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and NFAT localization.

Journal of immunology (Baltimore, Md. : 1950)

Weber KS,Hildner K,Murphy KM,Allen PM

Wed Sep 01 00:00:00 EDT 2010

Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation.

Journal of immunology (Baltimore, Md. : 1950)

Lal G,Zhang N,van der Touw W,Ding Y,Ju W,Bottinger EP,Reid SP,Levy DE,Bromberg JS

16-7041 was used in Functional assays to investigate Foxp3 as the master switch for Tregs, showing that expression is regulated by DNA methylation in Tregs.

Thu Jan 01 00:00:00 EST 2009

STAT6 deletion converts the Th2 inflammatory pathology afflicting Lat(Y136F) mice into a lymphoproliferative disorder involving Th1 and CD8 effector T cells.

Journal of immunology (Baltimore, Md. : 1950)

Archambaud C,Sansoni A,Mingueneau M,Devilard E,Delsol G,Malissen B,Malissen M

16-7041 was used in Flow cytometry/Cell sorting to study the effect of STAT6 delection on Lat(Y136F) mice.

Sun Mar 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: B-cell growth factor 1; B-cell IgG differentiation factor; B-cell stimulatory factor 1; BSF-1; H-IL-4; IGG1 induction factor; IL-4; ILN; Interleukin; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402

View more View less

Gene Aliases: BSF-1; Il-4; Il4

View more View less

UniProt ID: (Mouse) P07750

View more View less

Entrez Gene ID: (Mouse) 16189

View more View less

Function(s)

cytokine activity cytokine receptor binding interleukin-4 receptor binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-4 Monoclonal Antibody (11B11), Functional Grade, eBioscience™

IL-4 Antibody (16-7041-81) in IS

Product Details

16-7041-81

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Inhibition Assays (IA)

T-Cell Activation (TCA)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!