Invitrogen

KLRG1 Monoclonal Antibody (2F1), Biotin, eBioscience™

FIGURE: 1 / 17

KLRG1 Antibody (13-5893-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.06 µg of Golden Syrian Hamster IgG Isotype Control (Product # 13-4914-81) (left) or 0.06 µg of Anti-Mouse KLRG1 Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

13-5893-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 25 publications 25 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2F1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466770

Product Specific Information

Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.

Applications Reported: 2F1 has been reported for use in flow cytometric analysis.

Applications Tested: The 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Murine cytomegalovirus downregulates ERAAP and induces an unconventional T cell response to self.

Cell reports

Geiger KM,Manoharan M,Coombs R,Arana K,Park CS,Lee AY,Shastri N,Robey EA,Coscoy L

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Tue Apr 25 00:00:00 EDT 2023

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development.

Nature immunology

Scherer S,Oberle SG,Kanev K,Gerullis AK,Wu M,de Almeida GP,Puleston DJ,Baixauli F,Aly L,Greco A,Nizharadze T,Becker NB,Hoesslin MV,Donhauser LV,Berner J,Chu T,McNamara HA,Esencan Z,Roelli P,Wurmser C,Kleiter I,Vehreschild MJGT,Mayer CA,Knolle P,Klingenspor M,Fumagalli V,Iannacone M,Prlic M,Korn T,Pearce EL,Höfer T,Schulz AM,Zehn D

13-5893-82 was used in Flow cytometry/Cell sorting to show memory T cell precursors are resistant to pyrimidine starvation.

Wed Mar 01 00:00:00 EST 2023

Maturation and specialization of group 2 innate lymphoid cells through the lung-gut axis.

Nature communications

Zhao M,Shao F,Yu D,Zhang J,Liu Z,Ma J,Xia P,Wang S

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Fri Dec 09 00:00:00 EST 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Combination blockade of KLRG1 and PD-1 promotes immune control of local and disseminated cancers.

Oncoimmunology

Tata A,Dodard G,Fugère C,Leget C,Ors M,Rossi B,Vivier E,Brossay L

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Tue Jun 15 00:00:00 EDT 2021

Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.

JCI insight

Yun JH,Lee C,Liu T,Liu S,Kim EY,Xu S,Curtis JL,Pinello L,Bowler RP,Silverman EK,Hersh CP,Zhou X

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses.

The Journal of experimental medicine

Chen L,Christian DA,Kochanowsky JA,Phan AT,Clark JT,Wang S,Berry C,Oh J,Chen X,Roos DS,Beiting DP,Koshy AA,Hunter CA

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Cancer cells educate natural killer cells to a metastasis-promoting cell state.

The Journal of cell biology

Chan IS,Knútsdóttir H,Ramakrishnan G,Padmanaban V,Warrier M,Ramirez JC,Dunworth M,Zhang H,Jaffee EM,Bader JS,Ewald AJ

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Functional Assay

Mon Sep 07 00:00:00 EDT 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.

Oncoimmunology

Huang RY,Francois A,McGray AR,Miliotto A,Odunsi K

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Developmental Progression and Interrelationship of Central and Effector Regulatory T Cell Subsets.

Journal of immunology (Baltimore, Md. : 1950)

Toomer KH,Yuan X,Yang J,Dee MJ,Yu A,Malek TR

13-5893 was used in Flow cytometry/Cell sorting to elucidate the developmental progression and interrelationship of central and effector regulatory T cell subsets.

Sun May 01 00:00:00 EDT 2016

IL-15 signaling promotes adoptive effector T-cell survival and memory formation in irradiation-induced lymphopenia.

Cell & bioscience

Xu A,Bhanumathy KK,Wu J,Ye Z,Freywald A,Leary SC,Li R,Xiang J

13-5893 was used in Flow cytometry/Cell sorting to investigate the mechanism for adoptively transferred effector T-cell survival and memory formation.

Mon May 09 00:00:00 EDT 2016

MHC-dependent inhibition of uterine NK cells impedes fetal growth and decidual vascular remodelling.

Nature communications

Kieckbusch J,Gaynor LM,Moffett A,Colucci F

Published figure using KLRG1 monoclonal antibody (Product # 13-5893-82) in Flow Cytometry

Fri Feb 28 00:00:00 EST 2014

Markers of nonselective and specific NK cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Fogel LA,Sun MM,Geurs TL,Carayannopoulos LN,French AR

13-5893 was used in Flow cytometry/Cell sorting to demonstrate that a combination of Sca-1, CD27, and KLRG1 can distinguish NK cells based on their mechanism of activation.

Sat Jun 15 00:00:00 EDT 2013

Role for E-cadherin as an inhibitory receptor on epidermal gammadelta T cells.

Journal of immunology (Baltimore, Md. : 1950)

Uchida Y,Kawai K,Ibusuki A,Kanekura T

13-5893 was used in Flow cytometry/Cell sorting to analyse functional differences between E-cadherin and α(E)β(7) integrin expressed on murine dendritic epidermal T cells.

Wed Jun 15 00:00:00 EDT 2011

One naive T cell, multiple fates in CD8+ T cell differentiation.

The Journal of experimental medicine

Gerlach C,van Heijst JW,Swart E,Sie D,Armstrong N,Kerkhoven RM,Zehn D,Bevan MJ,Schepers K,Schumacher TN

13-5893 was used in Flow cytometry/Cell sorting to assess the potential for development of single naive T cells into different subsets.

Mon Jun 07 00:00:00 EDT 2010

IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.

Blood

Rubinstein MP,Lind NA,Purton JF,Filippou P,Best JA,McGhee PA,Surh CD,Goldrath AW

13-5893 was used in Flow cytometry/Cell sorting to study IL-7 and IL-15 regulation of CD8+ T-cell subsets during contraction of the immune response.

Sat Nov 01 00:00:00 EDT 2008

Homeostasis and effector function of lymphopenia-induced "memory-like" T cells in constitutively T cell-depleted mice.

Journal of immunology (Baltimore, Md. : 1950)

Voehringer D,Liang HE,Locksley RM

13-5893-82 was used in Flow Cytometry to demonstrate that T(ML) cells are not sufficient to mediate an effective immune response in constitutively T cell-depleted mice.

Tue Apr 01 00:00:00 EDT 2008

Unique receptor repertoire in mouse uterine NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Yadi H,Burke S,Madeja Z,Hemberger M,Moffett A,Colucci F

13-5893-82 was used in Flow Cytometry to define the receptor repertoire and phenotype of uterine NK cells at mid-gestation in mice.

Sat Nov 01 00:00:00 EDT 2008

Mechanisms involved in synergistic anticancer immunity of anti-4-1BB and anti-CD4 therapy.

Cancer research

Choi BK,Kim YH,Kang WJ,Lee SK,Kim KH,Shin SM,Yokoyama WM,Kim TY,Kwon BS

13-5893 was used in Flow cytometry/Cell sorting to demonstrate a therapy that combines the use of two mAbs for the enhanced production of efficient tumour-killing cytotoxic T lymphocytes.

Sat Sep 15 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Killer cell lectin-like receptor subfamily G member 1; Mast cell function-associated antigen 2F1; natural killer cell receptor

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Gene Aliases: 2F1-Ag; Klrg1; MAFA; MAFA-L

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UniProt ID: (Mouse) O88713

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Entrez Gene ID: (Mouse) 50928

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Function(s)

carbohydrate binding transmembrane signal receptor

Process(es)

immune system process cell surface receptor signaling pathway innate immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KLRG1 Monoclonal Antibody (2F1), Biotin, eBioscience™

KLRG1 Antibody (13-5893-82) in Flow

Product Details

13-5893-82

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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