Invitrogen

KLRG1 Monoclonal Antibody (2F1), PE-Cyanine7, eBioscience™

FIGURE: 1 / 17

KLRG1 Antibody (25-5893-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse NK1-1 FITC (Product # 11-5941-82) and 0.125 µg of Golden Syrian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4914-82) (left) or 0.125 µg of Anti-Mouse KLRG1 PE-Cyanine7 (right). Total viable cells were used for analysis.

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Product Details

25-5893-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2F1

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1518768

Product Specific Information

Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.

Applications Reported: This 2F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Murine cytomegalovirus downregulates ERAAP and induces an unconventional T cell response to self.

Cell reports

Geiger KM,Manoharan M,Coombs R,Arana K,Park CS,Lee AY,Shastri N,Robey EA,Coscoy L

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Tue Apr 25 00:00:00 EDT 2023

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

GSK-3 Inhibitor Elraglusib Enhances Tumor-Infiltrating Immune Cell Activation in Tumor Biopsies and Synergizes with Anti-PD-L1 in a Murine Model of Colorectal Cancer.

International journal of molecular sciences

Huntington KE,Louie AD,Srinivasan PR,Schorl C,Lu S,Silverberg D,Newhouse D,Wu Z,Zhou L,Borden BA,Giles FJ,Dooner M,Carneiro BA,El-Deiry WS

25-5893-82 was used in Flow cytometry/Cell sorting to investigate the immunomodulatory properties of the GSK-3 inhibitor elraglusib, revealing its capacity to enhance immune cell-mediated tumor cell killing, sensitize colorectal cancer cells to immune-mediated cytotoxicity, reduce immunosuppressive factors, and improve survival when combined with immune checkpoint blockade therapy in preclinical models and clinical samples.

Thu Jun 29 00:00:00 EDT 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Maturation and specialization of group 2 innate lymphoid cells through the lung-gut axis.

Nature communications

Zhao M,Shao F,Yu D,Zhang J,Liu Z,Ma J,Xia P,Wang S

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Fri Dec 09 00:00:00 EST 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Combination blockade of KLRG1 and PD-1 promotes immune control of local and disseminated cancers.

Oncoimmunology

Tata A,Dodard G,Fugère C,Leget C,Ors M,Rossi B,Vivier E,Brossay L

25-5893-82 was used in Flow Cytometry to demonstrate if killer cell lectin-like receptor G1 is a novel checkpoint inhibitor target that affects NK and T cell anti-tumor immunity.

Tue Jun 15 00:00:00 EDT 2021

Combination blockade of KLRG1 and PD-1 promotes immune control of local and disseminated cancers.

Oncoimmunology

Tata A,Dodard G,Fugère C,Leget C,Ors M,Rossi B,Vivier E,Brossay L

25-5893-82 was used in Flow Cytometry to demonstrate if killer cell lectin-like receptor G1 is a novel checkpoint inhibitor target that affects NK and T cell anti-tumor immunity.

Tue Jun 15 00:00:00 EDT 2021

Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.

JCI insight

Yun JH,Lee C,Liu T,Liu S,Kim EY,Xu S,Curtis JL,Pinello L,Bowler RP,Silverman EK,Hersh CP,Zhou X

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

Generating Mice with Diverse Microbial Experience.

Current protocols

Pierson M,Merley A,Hamilton SE

25-5893-82 was used in Flow cytometry/Cell sorting to outline procedures for exposing laboratory strains of mice to the diverse array of microbes typically found in pet store mice.

Mon Feb 01 00:00:00 EST 2021

Sex-specific adipose tissue imprinting of regulatory T cells.

Nature

Vasanthakumar A,Chisanga D,Blume J,Gloury R,Britt K,Henstridge DC,Zhan Y,Torres SV,Liene S,Collins N,Cao E,Sidwell T,Li C,Spallanzani RG,Liao Y,Beavis PA,Gebhardt T,Trevaskis N,Nutt SL,Zajac JD,Davey RA,Febbraio MA,Mathis D,Shi W,Kallies A

25-5893-82 was used in Flow Cytometry to find that sex-specific differences in Treg cells from visceral adipose tissue are determined by the tissue niche in a sex-hormone-dependent manner to limit adipose tissue inflammation.

Sun Mar 01 00:00:00 EST 2020

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses.

The Journal of experimental medicine

Chen L,Christian DA,Kochanowsky JA,Phan AT,Clark JT,Wang S,Berry C,Oh J,Chen X,Roos DS,Beiting DP,Koshy AA,Hunter CA

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Hhex Directly Represses BIM-Dependent Apoptosis to Promote NK Cell Development and Maintenance.

Cell reports

Goh W,Scheer S,Jackson JT,Hediyeh-Zadeh S,Delconte RB,Schuster IS,Andoniou CE,Rautela J,Degli-Esposti MA,Davis MJ,McCormack MP,Nutt SL,Huntington ND

25-5893 was used in Flow cytometry/Cell sorting to show homeobox transcriptional regulator Hhex regulates NK cell homeostasis and immunity.

Tue Oct 20 00:00:00 EDT 2020

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

25-5893 was used in Flow cytometry/Cell sorting to show homeostasis of thymically-derived and peripherally-derived Treg cells is maintained through the balancing of TCR signaling induced transcriptional activity by Bach2.

Tue Jan 14 00:00:00 EST 2020

Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells.

Cell reports

Dodard G,Tata A,Erick TK,Jaime D,Miah SMS,Quatrini L,Escalière B,Ugolini S,Vivier E,Brossay L

25-5893 was used in Flow cytometry/Cell sorting to characterize the kinetics and immune response of each of the hepatic NK cell populations to viral challenge and other stimuli.

Tue Jul 07 00:00:00 EDT 2020

Qa-1-Restricted CD8⁺ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection.

Cell reports

Anderson CK,Reilly EC,Lee AY,Brossay L

25-5893 was used in Flow cytometry/Cell sorting to study compensatory mechanisms in immune responses to viruses when classical T cell immunity has been evaded.

Tue Apr 09 00:00:00 EDT 2019

IL-33 Signaling Alters Regulatory T Cell Diversity in Support of Tumor Development.

Cell reports

Li A,Herbst RH,Canner D,Schenkel JM,Smith OC,Kim JY,Hillman M,Bhutkar A,Cuoco MS,Rappazzo CG,Rogers P,Dang C,Jerby-Arnon L,Rozenblatt-Rosen O,Cong L,Birnbaum M,Regev A,Jacks T

25-5893-82 was used in Flow Cytometry to profile dynamic shifts in the distribution of Tregs in a genetically engineered mouse model of lung adenocarcinoma.

Tue Dec 03 00:00:00 EST 2019

Blimp1 Prevents Methylation of Foxp3 and Loss of Regulatory T Cell Identity at Sites of Inflammation.

Cell reports

Garg G,Muschaweckh A,Moreno H,Vasanthakumar A,Floess S,Lepennetier G,Oellinger R,Zhan Y,Regen T,Hiltensperger M,Peter C,Aly L,Knier B,Palam LR,Kapur R,Kaplan MH,Waisman A,Rad R,Schotta G,Huehn J,Kallies A,Korn T

25-5893 was used in Flow cytometry/Cell sorting to identify a Blimp1-dependent pathway that preserves Treg cell stability in inflamed non-lymphoid tissues.

Tue Feb 12 00:00:00 EST 2019

Satb1 regulates the effector program of encephalitogenic tissue Th17 cells in chronic inflammation.

Nature communications

Yasuda K,Kitagawa Y,Kawakami R,Isaka Y,Watanabe H,Kondoh G,Kohwi-Shigematsu T,Sakaguchi S,Hirota K

25-5893-82 was used in Flow Cytometry to indicate that Satb1 regulates the specific gene expression and function of effector Th17 cells in tissue inflammation.

Fri Feb 01 00:00:00 EST 2019

CXCR6-Deficiency Improves the Control of Pulmonary Mycobacterium tuberculosis and Influenza Infection Independent of T-Lymphocyte Recruitment to the Lungs.

Frontiers in immunology

Ashhurst AS,Flórido M,Lin LCW,Quan D,Armitage E,Stifter SA,Stambas J,Britton WJ

25-5893 was used in Flow cytometry/Cell sorting to show that adoptive transfer of P25-specific CXCR6-deficient T-lymphocytes into WT mice revealed that this functional change in Th1-cytokine production was not due to a T-lymphocyte intrinsic mechanism.

Tue May 26 00:00:00 EDT 2020

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Cytokine- and TCR-Mediated Regulation of T Cell Expression of Ly6C and Sca-1.

Journal of immunology (Baltimore, Md. : 1950)

DeLong JH,Hall AO,Konradt C,Coppock GM,Park J,Harms Pritchard G,Hunter CA

25-5893-82 was used in Flow Cytometry to highlight the TCR-dependent and cytokine-mediated signals that modulate T cell expression of Ly6C and Sca-1 in vitro and in vivo during infection.

Thu Mar 01 00:00:00 EST 2018

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.

Oncoimmunology

Huang RY,Francois A,McGray AR,Miliotto A,Odunsi K

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

A Mouse Model of West Nile Virus Infection.

Current protocols in mouse biology

Graham JB,Swarts JL,Lund JM

25-5893 was used in Flow cytometry/Cell sorting to develop a mouse model to study the breadth of symptoms and disease severity seen in human West Nile virus infection.

Wed Dec 20 00:00:00 EST 2017

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using KLRG1 monoclonal antibody (Product # 25-5893-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

T Cell factor 1 represses CD8+ effector T cell formation and function.

Journal of immunology (Baltimore, Md. : 1950)

Tiemessen MM,Baert MR,Kok L,van Eggermond MC,van den Elsen PJ,Arens R,Staal FJ

25-5893 was used in Flow cytometry/Cell sorting to explore the role of the Wnt-responsive transcription factor T cell factor 1 in the initial phases of CD8(+) T effector cell formation.

Mon Dec 01 00:00:00 EST 2014

MHC-dependent inhibition of uterine NK cells impedes fetal growth and decidual vascular remodelling.

Nature communications

Kieckbusch J,Gaynor LM,Moffett A,Colucci F

25-5893 was used in Flow cytometry/Cell sorting to investigate MHC-dependent NK inhibition as a risk factor for pregnancy-related complications associated with impaired arterial remodelling.

Fri Feb 28 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Killer cell lectin-like receptor subfamily G member 1; Mast cell function-associated antigen 2F1; natural killer cell receptor

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Gene Aliases: 2F1-Ag; Klrg1; MAFA; MAFA-L

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UniProt ID: (Mouse) O88713

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Entrez Gene ID: (Mouse) 50928

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Function(s)

carbohydrate binding transmembrane signal receptor

Process(es)

immune system process cell surface receptor signaling pathway innate immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KLRG1 Monoclonal Antibody (2F1), PE-Cyanine7, eBioscience™

KLRG1 Antibody (25-5893-82) in Flow

Product Details

25-5893-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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