Invitrogen

KLRG1 Monoclonal Antibody (2F1), PerCP-eFluor™ 710, eBioscience™

FIGURE: 1 / 18

KLRG1 Antibody (46-5893-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.06 µg of Golden Syrian Hamster IgG Isotype Control PerCP-eFluor® 710 (Product # 46-4914-80) (left) or 0.06 µg of Anti-Mouse KLRG1 PerCP-eFluor® 710 (right). Total viable cells were used for analysis.

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Product Details

46-5893-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Syrian hamster / IgG

Recommended Isotype Control

Syrian Hamster IgG Isotype Control, PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2F1

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670282

Product Specific Information

Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.

Applications Reported: This 2F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2F1 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Murine cytomegalovirus downregulates ERAAP and induces an unconventional T cell response to self.

Cell reports

Geiger KM,Manoharan M,Coombs R,Arana K,Park CS,Lee AY,Shastri N,Robey EA,Coscoy L

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Tue Apr 25 00:00:00 EDT 2023

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

46-5893-82 was used in Flow Cytometry to establish both direct and indirect roles for ILC2 in peri-aortic CaPO4 injury-induced mouse abdominal aortic aneurysms.

Wed Mar 01 00:00:00 EST 2023

Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.

Cardiovascular research

Liu T,Meng Z,Liu J,Li J,Zhang Y,Deng Z,Luo S,Wang M,Huang Q,Zhang S,Fendt P,Devouassoux J,Li D,McKenzie ANJ,Nahrendorf M,Libby P,Guo J,Shi GP

46-5893-82 was used in Flow cytometry/Cell sorting to reveal an indirect cardiac reparative role of ILC2 in post-MI hearts via the IL5, EOS, and DC mechanism.

Tue May 02 00:00:00 EDT 2023

Maturation and specialization of group 2 innate lymphoid cells through the lung-gut axis.

Nature communications

Zhao M,Shao F,Yu D,Zhang J,Liu Z,Ma J,Xia P,Wang S

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Fri Dec 09 00:00:00 EST 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Combination blockade of KLRG1 and PD-1 promotes immune control of local and disseminated cancers.

Oncoimmunology

Tata A,Dodard G,Fugère C,Leget C,Ors M,Rossi B,Vivier E,Brossay L

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Tue Jun 15 00:00:00 EDT 2021

Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.

JCI insight

Yun JH,Lee C,Liu T,Liu S,Kim EY,Xu S,Curtis JL,Pinello L,Bowler RP,Silverman EK,Hersh CP,Zhou X

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

Mitochondrial transcription factor A in RORγt⁺ lymphocytes regulate small intestine homeostasis and metabolism.

Nature communications

Fu Z,Dean JW,Xiong L,Dougherty MW,Oliff KN,Chen ZE,Jobin C,Garrett TJ,Zhou L

46-5893-82 was used in Flow Cytometry to show that IL-22, a cytokine produced by RORγt+ lymphocytes inhibits IL-13-induced tuft cell differentiation in vitro, and suppresses the tuft cell-type 2 immune circuit and small intestine lengthening in vivo, highlighting its key role in gut tissue remodeling.

Thu Jul 22 00:00:00 EDT 2021

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

46-5893 was used in Flow cytometry/Cell sorting to suggest that serum amyloid A proteins direct a pathogenic pro-inflammatory Th17 cell differentiation program and promote Th17-mediated inflammatory diseases.

Thu Jan 09 00:00:00 EST 2020

Quorum Regulation via Nested Antagonistic Feedback Circuits Mediated by the Receptors CD28 and CTLA-4 Confers Robustness to T Cell Population Dynamics.

Immunity

Zenke S,Palm MM,Braun J,Gavrilov A,Meiser P,Böttcher JP,Beyersdorf N,Ehl S,Gerard A,Lämmermann T,Schumacher TN,Beltman JB,Rohr JC

46-5893 was used in Flow cytometry/Cell sorting to conclude that CD8+ T cell-population-intrinsic mechanisms regulate cellular behavior, thereby promoting robustness of population dynamics.

Tue Feb 18 00:00:00 EST 2020

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses.

The Journal of experimental medicine

Chen L,Christian DA,Kochanowsky JA,Phan AT,Clark JT,Wang S,Berry C,Oh J,Chen X,Roos DS,Beiting DP,Koshy AA,Hunter CA

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

46-5893 was used in Flow cytometry/Cell sorting to provide a framework for effective vaccination against cancer-associated herpesviruses through the elimination of latency and key immune evasion mechanisms from the pathogen.

Thu Nov 05 00:00:00 EST 2020

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

46-5893 was used in Flow cytometry/Cell sorting to identify a discrete ILC2-committed population and delineated transition states between early progenitors and a highly heterogeneous ILC1, ILC3, and NK precursor cell cluster.

Tue Jul 16 00:00:00 EDT 2019

Characterization of Transcriptional Regulatory Networks that Promote and Restrict Identities and Functions of Intestinal Innate Lymphoid Cells.

Immunity

Pokrovskii M,Hall JA,Ochayon DE,Yi R,Chaimowitz NS,Seelamneni H,Carriero N,Watters A,Waggoner SN,Littman DR,Bonneau R,Miraldi ER

46-5893 was used in Flow cytometry/Cell sorting to study regulatory interactions between transcription factors and genes within intestinal type 1, 2, and 3 innate lymphoid cells subsets.

Tue Jul 16 00:00:00 EDT 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

MicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity.

Frontiers in immunology

Knolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Interleukin-33-Activated Islet-Resident Innate Lymphoid Cells Promote Insulin Secretion through Myeloid Cell Retinoic Acid Production.

Immunity

Dalmas E,Lehmann FM,Dror E,Wueest S,Thienel C,Borsigova M,Stawiski M,Traunecker E,Lucchini FC,Dapito DH,Kallert SM,Guigas B,Pattou F,Kerr-Conte J,Maechler P,Girard JP,Konrad D,Wolfrum C,Böni-Schnetzler M,Finke D,Donath MY

46-5893 was used in Flow cytometry/Cell sorting to provide evidence that an immunometabolic crosstalk between islet-derived IL-33, ILC2s, and myeloid cells fosters insulin secretion.

Tue Nov 21 00:00:00 EST 2017

Epithelial-Cell-Derived Phospholipase A₂ Group 1B Is an Endogenous Anthelmintic.

Cell host & microbe

Entwistle LJ,Pelly VS,Coomes SM,Kannan Y,Perez-Lloret J,Czieso S,Silva Dos Santos M,MacRae JI,Collinson L,Sesay A,Nikolov N,Metidji A,Helmby H,Hui DY,Wilson MS

46-5893 was used in Flow cytometry/Cell sorting to uncover epithelial-cell-derived Pla2g1b as an essential mediator of helminth killing.

Wed Oct 11 00:00:00 EDT 2017

Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy.

Cell stem cell

Lechner AJ,Driver IH,Lee J,Conroy CM,Nagle A,Locksley RM,Rock JR

46-5893 was used in Flow cytometry/Cell sorting to provide evidence that group 2 innate lymphoid cells are a source of IL-13, which promotes lung regeneration.

Thu Jul 06 00:00:00 EDT 2017

Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.

Oncoimmunology

Huang RY,Francois A,McGray AR,Miliotto A,Odunsi K

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Modeling the dynamics of neonatal CD8⁺ T-cell responses.

Immunology and cell biology

Reynaldi A,Smith NL,Schlub TE,Venturi V,Rudd BD,Davenport MP

46-5893 was used in Flow cytometry/Cell sorting to analyse the dynamics of neonatal and adult CD8 T cell responses following in vitro stimulation and in vivo infection.

Sat Oct 01 00:00:00 EDT 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

MHC-dependent inhibition of uterine NK cells impedes fetal growth and decidual vascular remodelling.

Nature communications

Kieckbusch J,Gaynor LM,Moffett A,Colucci F

Published figure using KLRG1 monoclonal antibody (Product # 46-5893-82) in Flow Cytometry

Fri Feb 28 00:00:00 EST 2014

Type 2 innate lymphoid cells control eosinophil homeostasis.

Nature

Nussbaum JC,Van Dyken SJ,von Moltke J,Cheng LE,Mohapatra A,Molofsky AB,Thornton EE,Krummel MF,Chawla A,Liang HE,Locksley RM

46-5893 was used in Flow cytometry/Cell sorting to investigate the processes underlying regulation of cytokine and chemokines for eosinophil development and survival, showing that type 2 innate lymphoid cells control eosinophil homeostasis.

Thu Oct 10 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Killer cell lectin-like receptor subfamily G member 1; Mast cell function-associated antigen 2F1; natural killer cell receptor

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Gene Aliases: 2F1-Ag; Klrg1; MAFA; MAFA-L

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UniProt ID: (Mouse) O88713

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Entrez Gene ID: (Mouse) 50928

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Function(s)

carbohydrate binding transmembrane signal receptor

Process(es)

immune system process cell surface receptor signaling pathway innate immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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KLRG1 Monoclonal Antibody (2F1), PerCP-eFluor™ 710, eBioscience™

KLRG1 Antibody (46-5893-82) in Flow

Product Details

46-5893-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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