Invitrogen

mCherry Monoclonal Antibody (16D7), Alexa Fluor™ 488

mCherry Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

mCherry Antibody (M11239) in Flow

Samples were acquired and analyzed using 488 nm excitation and 530/30 nm band pass emission filters on an Attune® Acoustic Focusing Cytometer. The histogram shows cells stained with mCherry Rat Monoclonal Ab Alexa Fluor® 488 Conjugate.

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Product Details

M11239

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

16D7

Immunogen

Full-length mCherry was used as the immunogen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_2536613

Target Information

MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

A DNA Vaccine in Which the RSV-F Ectodomain Is Covalently Linked to the Burkholderia pseudomallei Antigens TssM and Hcp1 Augments the Humoral and Cytotoxic Response in Mice.

Frontiers in immunology

Solodushko V,Bitko V,Barrington R,Fouty B

M11239 was used in Flow cytometry/Cell sorting to demonstrate that a DNA vaccine in which the RSV-F ectodomain is fused to two antigens expressed by Burkholderia pseudomallei, the intracellular gram-negative organism that causes melioidosis, generated a stronger cytotoxic response than a DNA vaccine that lacked the RSV-F sequence while still generating a robust humoral response.

Thu Oct 29 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: mCherry

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