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Invitrogen
mCherry Monoclonal Antibody (16D7), Alexa Fluor™ 647
FIGURE: 1 / 3
mCherry Antibody (M11241) in ICC/IF
Immunofluorescent analysis of mCherry Tag was performed using 70% confluent mCherry-H3 transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with mCherry Monoclonal Antibody (16D7), Alexa Fluor 647 (Product # M11241) at 1:100 dilution and Histone H3 Recombinant Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then l... View More
Product Details
M11241
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Published species
Tag
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
16D7
Immunogen
Full-length mCherry was used as the immunogen
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage conditions
4° C
Shipping conditions
Ambient
RRID
AB_2536615
Target Information
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mCherry
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