Invitrogen

mCherry Monoclonal Antibody (16D7), Alexa Fluor™ 594

mCherry Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 14

mCherry Antibody (M11240) in Flow

Samples were acquired and analyzed using 561 nm excitation specified band pass emission filter on a BD LSR II flow cytometer. The histogram shows cells stained with mCherry Rat Monoclonal Ab Alexa Fluor® 594 Conjugate.

mCherry Antibody in Immunocytochemistry (ICC/IF)

mCherry Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

mCherry Antibody in Immunohistochemistry - Free Floating (IHC (Free))

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Product Details

M11240

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

16D7

Immunogen

Full-length mCherry was used as the immunogen

Conjugate

Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594

Excitation/Emission Max

590/618 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_2536614

Target Information

MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of ATM-directed antiviral responses by HIV-1 Vif.

PLoS pathogens

Wong HT,Luperchio AM,Riley S,Salamango DJ

M11240 was used in Immunocytochemistry to demonstrate that the HIV-1 accessory protein Vif blocks ATM-directed DNA repair processes, activation of NF-κB signaling responses, and TRIM protein phosphorylation.

Fri Sep 01 00:00:00 EDT 2023

CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization.

Nature

Qian K,Tol MJ,Wu J,Uchiyama LF,Xiao X,Cui L,Bedard AH,Weston TA,Rajendran PS,Vergnes L,Shimanaka Y,Yin Y,Jami-Alahmadi Y,Cohn W,Bajar BT,Lin CH,Jin B,DeNardo LA,Black DL,Whitelegge JP,Wohlschlegel JA,Reue K,Shivkumar K,Chen FJ,Young SG,Li P,Tontonoz P

M11240 was used in Immunocytochemistry to define a molecular mechanism that regulates lipid droplet form and function to facilitate lipid utilization in thermogenic adipocytes.

Sun Jan 01 00:00:00 EST 2023

Circadian clock regulator Bmal1 gates axon regeneration via Tet3 epigenetics in mouse sensory neurons.

Nature communications

Halawani D,Wang Y,Ramakrishnan A,Estill M,He X,Shen L,Friedel RH,Zou H

M11240 was used in Immunohistochemistry to demonstrate that targeting Bmal1 enhances axon regeneration.

Thu Aug 24 00:00:00 EDT 2023

Distribution of microglia/immune cells in the brain of adult zebrafish in homeostatic and regenerative conditions: Focus on oxidative stress during brain repair.

The Journal of comparative neurology

Narra SS,Rondeau P,Fernezelian D,Gence L,Ghaddar B,Bourdon E,Lefebvre d'Hellencourt C,Rastegar S,Diotel N

M11240 was used in flow cytometry to provide a better characterization of microglia/immune cell distribution in the healthy zebrafish brain, highlighting some evolutionarily conserved features with mammals.

Wed Feb 01 00:00:00 EST 2023

Spatiotemporal dynamics of noradrenaline during learned behaviour.

Nature

Breton-Provencher V,Drummond GT,Feng J,Li Y,Sur M

M11240 was used in flow cytometry to show that LC-NA activity displays distinct spatiotemporal dynamics to enable two functions during learned behaviour: facilitating task execution and encoding reinforcement to improve performance accuracy.

Wed Jun 01 00:00:00 EDT 2022

Decoupling SARS-CoV-2 ORF6 localization and interferon antagonism.

Journal of cell science

Wong HT,Cheung V,Salamango DJ

M11240 was used in Immunocytochemistry to show that ORF6 localization and IFN antagonism are independent activities, which raises the possibility that ORF6 may have additional functions within membrane networks to enhance virus replication.

Tue Mar 15 00:00:00 EDT 2022

NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice.

eLife

Fergani C,Leon S,Padilla SL,Verstegen AM,Palmiter RD,Navarro VM

Published figure using mCherry monoclonal antibody (Product # M11240) in Immunohistochemistry

Wed Dec 19 00:00:00 EST 2018

Primary cilia are not calcium-responsive mechanosensors.

Nature

Delling M,Indzhykulian AA,Liu X,Li Y,Xie T,Corey DP,Clapham DE

M11240 was used in immunohistochemistry - frozen section to study primary cilia and their lack of calcium-responsive mechanosensors

Thu Mar 31 00:00:00 EDT 2016

Primary cilia are not calcium-responsive mechanosensors.

Nature

Delling M,Indzhykulian AA,Liu X,Li Y,Xie T,Corey DP,Clapham DE

M11240 was used in immunohistochemistry - frozen section to study primary cilia and their lack of calcium-responsive mechanosensors

Thu Mar 31 00:00:00 EDT 2016

Primary cilia are not calcium-responsive mechanosensors.

Nature

Delling M,Indzhykulian AA,Liu X,Li Y,Xie T,Corey DP,Clapham DE

M11240 was used in immunohistochemistry - frozen section to study primary cilia and their lack of calcium-responsive mechanosensors

Thu Mar 31 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: mCherry

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mCherry Monoclonal Antibody (16D7), Alexa Fluor™ 594

mCherry Antibody (M11240) in Flow

Product Details

M11240

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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