Invitrogen

CD8 Monoclonal Antibody (3B5), Alexa Fluor™ 405

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CD8 Antibody (MHCD0826) in Flow

Clinical immunology (Orlando, Fla.) 2014 - Published figure using CD8 monoclonal antibody (Product # MHCD0826) in Flow Cytometry

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Product Details

MHCD0826

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Recognizes the CD8 antigen

Conjugate

Alexa Fluor™ 405 Alexa Fluor™ 405 Alexa Fluor™ 405

Excitation/Emission Max

401/422 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372951

Product Specific Information

For fluorochrome conjugated antibodies only, light exposure should be avoided. Use dim light during handling, incubation with cells, and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Co-immunization of DNA and Protein in the Same Anatomical Sites Induces Superior Protective Immune Responses against SHIV Challenge.

Cell reports

Felber BK,Lu Z,Hu X,Valentin A,Rosati M,Remmel CAL,Weiner JA,Carpenter MC,Faircloth K,Stanfield-Oakley S,Williams WB,Shen X,Tomaras GD,LaBranche CC,Montefiori D,Trinh HV,Rao M,Alam MS,Vandergrift NA,Saunders KO,Wang Y,Rountree W,Das J,Alter G,Reed SG,Aye PP,Schiro F,Pahar B,Dufour JP,Veazey RS,Marx PA,Venzon DJ,Shaw GM,Ferrari G,Ackerman ME,Haynes BF,Pavlakis GN

MHCD0826 was used in Flow cytometry/Cell sorting to compare immunogenicity and protective efficacy of an HIV vaccine comprised of env and gag DNA and Env (Envelope) proteins by co-administration of the vaccine components in the same muscles or by separate administration of DNA + protein in contralateral sites.

Tue May 12 00:00:00 EDT 2020

Gag and env conserved element CE DNA vaccines elicit broad cytotoxic T cell responses targeting subdominant epitopes of HIV and SIV Able to recognize virus-infected cells in macaques.

Human vaccines & immunotherapeutics

Hu X,Lu Z,Valentin A,Rosati M,Broderick KE,Sardesai NY,Marx PA,Mullins JI,Pavlakis GN,Felber BK

MHCD0826 was used in Flow cytometry/Cell sorting to study the cytotoxic T cell responses against conserved protein segments of SIV Gag and HIV Env following DNA vaccination.

Mon May 06 00:00:00 EDT 2019

Triggering of TLR-3, -4, NOD2, and DC-SIGN reduces viral replication and increases T-cell activation capacity of HIV-infected human dendritic cells.

European journal of immunology

Cardinaud S,Urrutia A,Rouers A,Coulon PG,Kervevan J,Richetta C,Bet A,Maze EA,Larsen M,Iglesias MC,Appay V,Graff-Dubois S,Moris A

MHCD0826 was used in flow cytometry to elucidate the effects of pathogen recognition receptors on dendritic cell maturation, HIV infection, and on the quality of HIV-specific cytotoxic T-cell activation

Mon May 01 00:00:00 EDT 2017

CD8⁺ T Cells from Human Neonates Are Biased toward an Innate Immune Response.

Cell reports

Galindo-Albarrán AO,López-Portales OH,Gutiérrez-Reyna DY,Rodríguez-Jorge O,Sánchez-Villanueva JA,Ramírez-Pliego O,Bergon A,Loriod B,Holota H,Imbert J,Hernández-Mendoza A,Ferrier P,Carrillo-de Santa Pau E,Valencia A,Spicuglia S,Santana MA

MHCD0826 was used in flow cytometry to demonstrate that neonatal CD8 positive T cells have a specific genetic program biased toward the innate immune response

Tue Nov 15 00:00:00 EST 2016

Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques.

Clinical immunology (Orlando, Fla.)

Valentin A,McKinnon K,Li J,Rosati M,Kulkarni V,Pilkington GR,Bear J,Alicea C,Vargas-Inchaustegui DA,Jean Patterson L,Pegu P,Liyanage NPM,Gordon SN,Vaccari M,Wang Y,Hogg AE,Frey B,Sui Y,Reed SG,Sardesai NY,Berzofsky JA,Franchini G,Robert-Guroff M,Felber BK,Pavlakis GN

MHCD0826 was used in immunocytochemistry to compare five SIV vaccine platforms

Sat Nov 01 00:00:00 EDT 2014

Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques.

Clinical immunology (Orlando, Fla.)

MHCD0826 was used in immunocytochemistry to compare five SIV vaccine platforms

Sat Nov 01 00:00:00 EDT 2014

Syncytin proteins incorporated in placenta exosomes are important for cell uptake and show variation in abundance in serum exosomes from patients with preeclampsia.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Vargas A,Zhou S,Éthier-Chiasson M,Flipo D,Lafond J,Gilbert C,Barbeau B

MHCD0826 was used in flow cytometry to study the role of human endogenous retrovirus (HERV) envelope proteins in the uptake of exosomes

Fri Aug 01 00:00:00 EDT 2014

DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques.

Journal of medical primatology

Kulkarni V,Rosati M,Jalah R,Ganneru B,Alicea C,Yu L,Guan Y,LaBranche C,Montefiori DC,King AD,Valentin A,Pavlakis GN,Felber BK

MHCD0826 was used in Flow cytometry/Cell sorting to investigate how vaccination by an intradermal DNA delivery regimen is able to induce potent and durable immune responses in macaques.

Wed Oct 01 00:00:00 EDT 2014

Altered response hierarchy and increased T-cell breadth upon HIV-1 conserved element DNA vaccination in macaques.

PloS one

Kulkarni V,Valentin A,Rosati M,Alicea C,Singh AK,Jalah R,Broderick KE,Sardesai NY,Le Gall S,Mothe B,Brander C,Rolland M,Mullins JI,Pavlakis GN,Felber BK

MHCD0826 was used in flow cytometry to compare vaccination with highly conserved gag elements verses full-length gag DNA.

Tue Nov 11 00:00:00 EST 2014

IL-12 DNA as molecular vaccine adjuvant increases the cytotoxic T cell responses and breadth of humoral immune responses in SIV DNA vaccinated macaques.

Human vaccines & immunotherapeutics

Jalah R,Patel V,Kulkarni V,Rosati M,Alicea C,Ganneru B,von Gegerfelt A,Huang W,Guan Y,Broderick KE,Sardesai NY,LaBranche C,Montefiori DC,Pavlakis GN,Felber BK

MHCD0826 was used in flow cytometry to study the use of an IL-2 adjuvant when vaccinating rhesus macaques.

Thu Nov 01 00:00:00 EDT 2012

Emergence of simian immunodeficiency virus-specific cytotoxic CD4+ T cells and increased humoral responses correlate with control of rebounding viremia in CD8-depleted macaques infected with Rev-independent live-attenuated simian immunodeficiency virus.

Journal of immunology (Baltimore, Md. : 1950)

von Gegerfelt A,Valentin A,Alicea C,Van Rompay KK,Marthas ML,Montefiori DC,Pavlakis GN,Felber BK

MHCD0826 was used in flow cytometry to investigate immune mechanisms responsible for viral control in Indian rhesus macaques infected SIV

Wed Sep 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8 Monoclonal Antibody (3B5), Alexa Fluor™ 405

CD8 Antibody (MHCD0826) in Flow

Product Details

MHCD0826

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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