Invitrogen

CD8 Monoclonal Antibody (3B5), Qdot™ 605

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CD8 Antibody (Q10009) in Flow

Qdot® 605 anti-human CD8 conjugate 605/20 band pass filter

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Product Details

Q10009

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 16 publications 16 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Recognizes the CD8 antigen

Conjugate

Qdot™ 605 Qdot™ 605 Qdot™ 605

Excitation/Emission Max

300/603 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556437

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Neoantigen-reactive CD8+ T cells affect clinical outcome of adoptive cell therapy with tumor-infiltrating lymphocytes in melanoma.

The Journal of clinical investigation

Kristensen NP,Heeke C,Tvingsholm SA,Borch A,Draghi A,Crowther MD,Carri I,Munk KK,Holm JS,Bjerregaard AM,Bentzen AK,Marquard AM,Szallasi Z,McGranahan N,Andersen R,Nielsen M,Jönsson GB,Donia M,Svane IM,Hadrup SR

Q10009 was used in flow cytometry experiments to find that the frequency of neoepitope-specific CD8+ T cells in TIL infusion products correlated with increased survival and that neoepitope-specific CD8+ T cells shared with the infusion product in posttreatment blood samples were unique to responders of TIL-ACT.

Tue Jan 18 00:00:00 EST 2022

A phase 1/2 trial of an immune-modulatory vaccine against IDO/PD-L1 in combination with nivolumab in metastatic melanoma.

Nature medicine

Kjeldsen JW,Lorentzen CL,Martinenaite E,Ellebaek E,Donia M,Holmstroem RB,Klausen TW,Madsen CO,Ahmed SM,Weis-Banke SE,Holmström MO,Hendel HW,Ehrnrooth E,Zocca MB,Pedersen AW,Andersen MH,Svane IM

Q10009 was used in Immunocytochemistry to test a first-in-class immune-modulatory vaccine (IO102/IO103) against indoleamine 2,3-dioxygenase (IDO) and PD ligand 1 (PD-L1), targeting immunosuppressive cells and tumor cells expressing IDO and/or PD-L1 (IDO/PD-L1), combined with nivolumab.

Wed Dec 01 00:00:00 EST 2021

The role of protease-activated receptor 1 signaling in CD8 T cell effector functions.

iScience

Chen H,Smith M,Herz J,Li T,Hasley R,Le Saout C,Zhu Z,Cheng J,Gronda A,Martina JA,Irusta PM,Karpova T,McGavern DB,Catalfamo M

Q10009 was used in Flow Cytometry to show that in human CD8 T cells, PAR1 stimulation accelerates calcium mobilization and is involved in cytotoxic T cell function by facilitating granule trafficking via actin polymerization and repositioning of the microtubule organizing center toward the immunological synapse.

Fri Nov 19 00:00:00 EST 2021

MAIT cell activation is associated with disease severity markers in acute hantavirus infection.

Cell reports. Medicine

Maleki KT,Tauriainen J,García M,Kerkman PF,Christ W,Dias J,Wigren Byström J,Leeansyah E,Forsell MN,Ljunggren HG,Ahlm C,Björkström NK,Sandberg JK,Klingström J

Q10009 was used in Flow cytometry/Cell sorting to show the role of type I IFNs in virus-driven MAIT cell activation and suggest a potential role of MAIT cells in the disease pathogenesis of viral infections.

Tue Mar 16 00:00:00 EDT 2021

Antigen-Specific CD8 Lytic Phenotype Induced by Sipuleucel-T in Hormone-Sensitive or Castration-Resistant Prostate Cancer and Association with Overall Survival.

Clinical cancer research : an official journal of the American Association for Cancer Research

Antonarakis ES,Small EJ,Petrylak DP,Quinn DI,Kibel AS,Chang NN,Dearstyne E,Harmon M,Campogan D,Haynes H,Vu T,Sheikh NA,Drake CG

Q10009 was used in Flow cytometry/Cell sorting to study the mechanism by which Sipuleucel-T carries out its anticancer efficacy in metastatic castration-resistant prostate cancer.

Mon Oct 01 00:00:00 EDT 2018

T cell phenotypes in women with Chlamydia trachomatis infection and influence of treatment on phenotype distributions.

Microbes and infection

Ogendi BMO,Bakshi RK,Gupta K,Kapil R,Brown LT,Jordan SJ,Sabbaj S,Press CG,Lee JY,Geisler WM

Q10009 was used in Flow cytometry/Cell sorting to evaluate differences in T cell phenotypes between Chlamydia trachomatis(CT)-infected women and CT-seronegative controls and investigate changes in T cell phenotype distributions after CT treatment and their association with reinfection.

Thu Mar 01 00:00:00 EST 2018

Distinct peripheral vs mucosal T-cell phenotypes in chlamydia-infected women.

American journal of reproductive immunology (New York, N.Y. : 1989)

Ogendi BMO,Bakshi RK,Sabbaj S,Brown L,Lee JY,Kapil R,Geisler WM

Q10009 was used in Flow cytometry/Cell sorting to characterise the distinct peripheral and mucosal T-cell phenotypes in chlamydia-infected women.

Fri Dec 01 00:00:00 EST 2017

TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.

PLoS pathogens

Chew GM,Fujita T,Webb GM,Burwitz BJ,Wu HL,Reed JS,Hammond KB,Clayton KL,Ishii N,Abdel-Mohsen M,Liegler T,Mitchell BI,Hecht FM,Ostrowski M,Shikuma CM,Hansen SG,Maurer M,Korman AJ,Deeks SG,Sacha JB,Ndhlovu LC

Q10009 was used in Flow cytometry/Cell sorting to study the potential of TIGIT as a novel marker of dysfunctional HIV-specific T cells..

Fri Jan 01 00:00:00 EST 2016

γδ T cells and CD14+ monocytes are predominant cellular sources of cytokines and chemokines associated with severe malaria.

The Journal of infectious diseases

Stanisic DI,Cutts J,Eriksson E,Fowkes FJ,Rosanas-Urgell A,Siba P,Laman M,Davis TM,Manning L,Mueller I,Schofield L

Q10009 was used in flow cytometry to investigate the cellular sources of cytokines and chemokines associated with severe malaria

Tue Jul 15 00:00:00 EDT 2014

Subinfectious hepatitis C virus exposures suppress T cell responses against subsequent acute infection.

Nature medicine

Park SH,Veerapu NS,Shin EC,Biancotto A,McCoy JP,Capone S,Folgori A,Rehermann B

Q10009 was used in flow cytometry to decipher how susequent acute infection is supressed in T cell responses after exposure to subinfectious hepatitis C virus

Sun Dec 01 00:00:00 EST 2013

Simultaneous TCR and CD244 signals induce dynamic downmodulation of CD244 on human antiviral T cells.

Journal of immunology (Baltimore, Md. : 1950)

Pacheco Y,McLean AP,Rohrbach J,Porichis F,Kaufmann DE,Kavanagh DG

Q10009 was used in Flow cytometry/Cell sorting to reveal a two-signal mechanism inducing CD244 internalisation, with a role in modulation of antiviral immune response.

Sun Sep 01 00:00:00 EDT 2013

Expression profile of host restriction factors in HIV-1 elite controllers.

Retrovirology

Abdel-Mohsen M,Raposo RA,Deng X,Li M,Liegler T,Sinclair E,Salama MS,Ghanem Hel-D,Hoh R,Wong JK,David M,Nixon DF,Deeks SG,Pillai SK

Q10009 was used in flow cytometry to investigate cellular restriction of HIV-1

Wed Oct 16 00:00:00 EDT 2013

The contribution of non-conventional T cells and NK cells in the mycobacterial-specific IFNγ response in Bacille Calmette-Guérin (BCG)-immunized infants.

PloS one

Zufferey C,Germano S,Dutta B,Ritz N,Curtis N

Q10009 was used in flow cytometry to discuss the roles of TCR-gamma/delta T cells and natural killer cells in the mycobacterial-specific immune response

Tue Apr 29 00:00:00 EDT 2014

Leukocyte composition of human breast cancer.

Proceedings of the National Academy of Sciences of the United States of America

Ruffell B,Au A,Rugo HS,Esserman LJ,Hwang ES,Coussens LM

Q10009 was used in flow cytometry to examine tumor and nonadjacent normal breast tissue from women with breast cancer, who either had or had not received neoadjuvant chemotherapy before surgery

Tue Feb 21 00:00:00 EST 2012

CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.

Journal of immunology (Baltimore, Md. : 1950)

Frahm MA,Picking RA,Kuruc JD,McGee KS,Gay CL,Eron JJ,Hicks CB,Tomaras GD,Ferrari G

Q10009 was used in flow cytometry to analyze the proliferation and functional profile of circulating double positive T cells from acutely HIV-infected individuals and chronically HIV-infected viral controllers.

Tue May 01 00:00:00 EDT 2012

DNA-based HIV vaccines do not induce generalized activation in mucosal tissue T cells.

Human vaccines & immunotherapeutics

Reuter MA,Yuan S,Marx PA,Kutzler MA,Weiner DB,Betts MR

Q10009 was used in flow cytometry to test if vaccination alters the activation state of T cells within the gastrointestinal mucosa, inguinal lymph nodes, and peripheral blood

Thu Nov 01 00:00:00 EDT 2012

Increased frequency of regulatory T cells accompanies increased immune activation in rectal mucosae of HIV-positive noncontrollers.

Journal of virology

Shaw JM,Hunt PW,Critchfield JW,McConnell DH,Garcia JC,Pollard RB,Somsouk M,Deeks SG,Shacklett BL

Q10009 was used in flow cytometry to study the role of T regulatory cells in HIV infection

Tue Nov 01 00:00:00 EDT 2011

Delaying BCG vaccination from birth to 10 weeks of age may result in an enhanced memory CD4 T cell response.

Vaccine

Kagina BM,Abel B,Bowmaker M,Scriba TJ,Gelderbloem S,Smit E,Erasmus M,Nene N,Walzl G,Black G,Hussey GD,Hesseling AC,Hanekom WA

Q10009 was used in flow cytometry to test if delaying bacillus Calmette-Guerin vaccination from birth to 10 weeks of age enhances vaccine-induced immune responses

Fri Sep 04 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8 Monoclonal Antibody (3B5), Qdot™ 605

CD8 Antibody (Q10009) in Flow

Product Details

Q10009

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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