Invitrogen

CD8 Monoclonal Antibody (3B5), Qdot™ 705

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CD8 Antibody (Q10059) in Flow

Qdot® 705 anti-human CD8 conjugate 720/20 band pass filter

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Product Details

Q10059

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Recognizes the CD8 antigen

Conjugate

Qdot™ 705 Qdot™ 705 Qdot™ 705

Excitation/Emission Max

300/702 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11180330

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

IL-21 and IFNα therapy rescues terminally differentiated NK cells and limits SIV reservoir in ART-treated macaques.

Nature communications

Harper J,Huot N,Micci L,Tharp G,King C,Rascle P,Shenvi N,Wang H,Galardi C,Upadhyay AA,Villinger F,Lifson J,Silvestri G,Easley K,Jacquelin B,Bosinger S,Müller-Trutwin M,Paiardini M

Q10059 was used in Flow cytometry/Cell sorting to generate terminally differentiated blood natural killer cells (NKG2a/clowCD16+) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides.

Mon May 17 00:00:00 EDT 2021

A20 upregulation during treated HIV disease is associated with intestinal epithelial cell recovery and function.

PLoS pathogens

Chitre AS,Kattah MG,Rosli YY,Pao M,Deswal M,Deeks SG,Hunt PW,Abdel-Mohsen M,Montaner LJ,Kim CC,Ma A,Somsouk M,McCune JM

Q10059 was used in Flow cytometry/Cell sorting to show that variation in A20 expression during the course of HIV infection could underlie both the development of epithelial dysfunction before the initiation of ART and the recovery of intestinal epithelial integrity thereafter.

Thu Mar 01 00:00:00 EST 2018

Effect of Antiretroviral Therapy on the Memory and Activation Profiles of B Cells in HIV-Infected African Women.

Journal of immunology (Baltimore, Md. : 1950)

Tanko RF,Soares AP,Müller TL,Garrett NJ,Samsunder N,Abdool Karim Q,Abdool Karim SS,Riou C,Burgers WA

Q10059 was used in Flow cytometry/Cell sorting to study the effect of antiretroviral therapy on B cells in HIV infection.

Wed Feb 01 00:00:00 EST 2017

Loss of Function of Intestinal IL-17 and IL-22 Producing Cells Contributes to Inflammation and Viral Persistence in SIV-Infected Rhesus Macaques.

PLoS pathogens

Ryan ES,Micci L,Fromentin R,Paganini S,McGary CS,Easley K,Chomont N,Paiardini M

Q10059 was used in Flow cytometry/Cell sorting to show that intestinal IL-17 producing cell function is severely impaired by SIV infection and is not fully normalized despite effective ART.

Mon Feb 01 00:00:00 EST 2016

Selective reduction of IFN-γ single positive mycobacteria-specific CD4+ T cells in HIV-1 infected individuals with latent tuberculosis infection.

Tuberculosis (Edinburgh, Scotland)

Riou C,Bunjun R,Müller TL,Kiravu A,Ginbot Z,Oni T,Goliath R,Wilkinson RJ,Burgers WA

Q10059 was used in flow cytometry to explore how HIV-1 alters immunity during latent tuberculosis

Thu Dec 01 00:00:00 EST 2016

Differential Impact of In Vivo CD8+ T Lymphocyte Depletion in Controller versus Progressor Simian Immunodeficiency Virus-Infected Macaques.

Journal of virology

Chowdhury A,Hayes TL,Bosinger SE,Lawson BO,Vanderford T,Schmitz JE,Paiardini M,Betts M,Chahroudi A,Estes JD,Silvestri G

Q10059 was used in flow cytometry to elucidate how CD8 positive T cells suppress virus replication during simian immunodeficiency virus infection.

Tue Sep 01 00:00:00 EDT 2015

Quantification of Th1 and Th17 Cells with Intracellular Staining Following PMA/Ionomycin Stimulation.

Current protocols in cytometry

Karlsson F,Hassan-Zahraee M

Q10059 was used in flow cytometry to discuss methods to measure cytokine production by Th1 and Th17 cells

Mon Jan 05 00:00:00 EST 2015

Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.

The Journal of experimental medicine

Cichocki F,Schlums H,Li H,Stache V,Holmes T,Lenvik TR,Chiang SC,Miller JS,Meeths M,Anderson SK,Bryceson YT

Q10059 was used in flow cytometry to find Munc13-4 is highly expressed in differentiated human natural killer cells and effector CD8(+) T lymphocytes

Mon Jun 02 00:00:00 EDT 2014

Increased stability and limited proliferation of CD4+ central memory T cells differentiate nonprogressive simian immunodeficiency virus (SIV) infection of sooty mangabeys from progressive SIV infection of rhesus macaques.

Journal of virology

McGary CS,Cervasi B,Chahroudi A,Micci L,Taaffe J,Meeker T,Silvestri G,Davenport MP,Paiardini M

Q10059 was used in Flow cytometry/Cell sorting to provide new insights into the importance of long-term maintenance of CD4(+) TCM homeostasis during HIV/SIV infection.

Tue Apr 01 00:00:00 EDT 2014

CD3ζ-based chimeric antigen receptors mediate T cell activation via cis- and trans-signalling mechanisms: implications for optimization of receptor structure for adoptive cell therapy.

Clinical and experimental immunology

Bridgeman JS,Ladell K,Sheard VE,Miners K,Hawkins RE,Price DA,Gilham DE

Q10059 was used in flow cytometry to elucidate chimeric antigen receptor-mediated T cell activation

Sat Feb 01 00:00:00 EST 2014

Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.

PloS one

Deng N,Weaver JM,Mosmann TR

Q10059 was used in flow cytometry to investigate the anti-influenza responses of T cell subsets

Thu Dec 18 00:00:00 EST 2014

Immunological and virological analyses of rhesus macaques immunized with chimpanzee adenoviruses expressing the simian immunodeficiency virus Gag/Tat fusion protein and challenged intrarectally with repeated low doses of SIVmac.

Journal of virology

Cervasi B,Carnathan DG,Sheehan KM,Micci L,Paiardini M,Kurupati R,Tuyishime S,Zhou XY,Else JG,Ratcliffe SJ,Ertl HC,Silvestri G

Q10059 was used in flow cytometry to infect chimpanzee adenovirus-immunized rhesus macaques with simian immunodeficiency virus

Sun Sep 01 00:00:00 EDT 2013

Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.

Vaccine

Li X,Miao H,Henn A,Topham DJ,Wu H,Zand MS,Mosmann TR

Q10059 was used in flow cytometry to study influenza-specific CD4 T cells respond to vaccination using Ki67 as a marker.

Fri Jun 29 00:00:00 EDT 2012

CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.

Vaccine

Scheible K,Zhang G,Baer J,Azadniv M,Lambert K,Pryhuber G,Treanor JJ,Topham DJ

Q10059 was used in flow cytometry to examine human cross-reactive T cells against a pandemic virus.

Thu Mar 03 00:00:00 EST 2011

Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Chattopadhyay PK,Melenhorst JJ,Ladell K,Gostick E,Scheinberg P,Barrett AJ,Wooldridge L,Roederer M,Sewell AK,Price DA

Q10059 was used in flow cytometry to study low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class 1 tetramers and techniques to improve the direct ex vivo detection

Sat Nov 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8 Monoclonal Antibody (3B5), Qdot™ 705

CD8 Antibody (Q10059) in Flow

Product Details

Q10059

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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