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Invitrogen
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eBioscience™
FIGURE: 1 / 34
Mouse IgG1 kappa Isotype Control (14-4714-82) in ICC/IF
Immunocytochemistry of fixed and permeabilized human PBMC (stimulated with LPS and monensin for 5 hours) stained with 20 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 20 µg/mL of Anti-Human IL-37 (IL-1F7) Purified.Cells were then stained with F (ab')2 Anti-Mouse IgG eFluor® 570 followed by cytospin.Nuclei are stained with DAPI.
Product Details
14-4714-82
Applications
Tested Dilution
Western Blot (WB)
Assay-Dependent
Immunohistochemistry (Paraffin) (IHC (P))
Assay-Dependent
Immunocytochemistry (ICC/IF)
Assay-Dependent
Flow Cytometry (Flow)
Assay-Dependent
Immunoprecipitation (IP)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Isotype Control
Clone
P3.6.2.8.1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_470111
Product Specific Information
Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.
Applications Reported: This mouse IgG1 isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemistry, immunocytochemistry, immunoprecipitation and immunoblotting (WB).
Applications Tested: Mouse IgG1 K Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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