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Invitrogen
ROR gamma (t) Monoclonal Antibody (B2D), PE-Cyanine7, eBioscience™
FIGURE: 1 / 4
ROR gamma (t) Antibody (25-6981-82) in Flow
C57BL/6 mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD4 Monoclonal Antibody, FITC (Product # 11-0041-82) and 0.25 µg of Rat IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4301-82) (left) or 0.25 µg of ROR gamma (t) Monoclonal Antibody, PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
25-6981-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
B2D
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784671
Product Specific Information
Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.
Applications Reported: This B2D antibody has been reported for use in flow cytometric analysis.
Applications Tested: This B2D antibody has been tested by flow cytometric analysis of mouse thymocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.
ROR gammat t (RORgt) is an isoform of RORC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC129539; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORgt; RP11-98D18.11-001; Thymus orphan receptor; TOR
Gene Aliases: Nr1f3; Rorc; Rorg; RORgamma; Thor; TOR
UniProt ID: (Mouse) P51450
Entrez Gene ID: (Mouse) 19885
transcription coactivator binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
oxysterol binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
transcription factor activity, direct ligand regulated sequence-specific DNA binding
C4 zinc finger nuclear receptor
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
xenobiotic metabolic process
multicellular organism development
regulation of glucose metabolic process
regulation of steroid metabolic process
cell differentiation
interleukin-17 production
circadian regulation of gene expression
T cell differentiation in thymus
cellular response to sterol
T-helper cell differentiation
positive regulation of circadian rhythm
steroid hormone mediated signaling pathway
regulation of gamma-delta T cell differentiation
regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
alpha-beta T cell differentiation
rhythmic process
lymph node development
mucosal-associated lymphoid tissue development
Peyer's patch development
adipose tissue development
regulation of transcription involved in cell fate commitment
negative regulation of thymocyte apoptotic process
T-helper 17 cell differentiation
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