Invitrogen

ROR gamma (t) Monoclonal Antibody (B2D), PE-eFluor™ 610, eBioscience™

ROR gamma (t) Antibody in Flow Cytometry (Flow)

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ROR gamma (t) Antibody (61-6981-82) in Flow

Surface staining of mouse splenocytes with Anti-Mouse CD19 eFluor® 450 (Product # 48-0193-82), Anti-Mouse CD3 APC (Product # 17-0031-82), and Anti-Mouse CD4 FITC (Product # 11-0042-82), followed by intracellular staining with 0.125 µg of Rat IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4301-82) (left) or 0.125 µg of Anti-Mouse ROR gamma (t) PE-eFluor® 610 (right), using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Viable CD3. CD19. cells, as determined by Fixable Viability Dye eFluor® 780 (... View More

ROR gamma (t) Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

Product Details

61-6981-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B2D

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574650

Product Specific Information

Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

The ROR gamma t isoform differs from ROR gamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms.

Applications Reported: This B2D antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular protein under the Resources Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

ROR gammat t (RORgt) is an isoform of RORC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Protocol to assess cell-intrinsic regulatory mechanisms using an ex vivo murine T cell polarization and co-culture system.

STAR protocols

Ma S,Hernandez JE,Huang WJM

61-6981-82 was used in Flow Cytometry to generate a mixture of CD4+ T helper lineages, including Th17, RORγt+ Treg, and cTreg, in proportions resembling in vivo mucosal tissue conditions.

Fri Sep 16 00:00:00 EDT 2022

Dysbiosis exacerbates colitis by promoting ubiquitination and accumulation of the innate immune adaptor STING in myeloid cells.

Immunity

Shmuel-Galia L,Humphries F,Lei X,Ceglia S,Wilson R,Jiang Z,Ketelut-Carneiro N,Foley SE,Pechhold S,Houghton J,Muneeruddin K,Shaffer SA,McCormick BA,Reboldi A,Ward D,Marshak-Rothstein A,Fitzgerald KA

61-6981-82 was used in Flow cytometry/Cell sorting to suggest a positive feedback loop wherein dysbiosis foments the accumulation of STING in intestinal myeloid cells, driving intestinal inflammation.

Tue Jun 08 00:00:00 EDT 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

61-6981-82 was used in Flow cytometry/Cell sorting to demonstrate a critical role for antigen-presenting ILC3s in orchestrating immune tolerance in the airway by restricting pro-inflammatory T cell responses to both allergens and microbes.

Tue Nov 23 00:00:00 EST 2021

Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition.

Cell reports

Stephens WZ,Kubinak JL,Ghazaryan A,Bauer KM,Bell R,Buhrke K,Chiaro TR,Weis AM,Tang WW,Monts JK,Soto R,Ekiz HA,O'Connell RM,Round JL

61-6981-82 was used in Flow cytometry/Cell sorting to demonstrate a role for IEC-derived MHC class II in constraining microbiota composition and inducing tolerogenic responses against it.

Tue Nov 02 00:00:00 EDT 2021

Tipping the immunostimulatory and inhibitory DAMP balance to harness immunogenic cell death.

Nature communications

Hayashi K,Nikolos F,Lee YC,Jain A,Tsouko E,Gao H,Kasabyan A,Leung HE,Osipov A,Jung SY,Kurtova AV,Chan KS

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6981-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity.

Nature communications

Toubal A,Kiaf B,Beaudoin L,Cagninacci L,Rhimi M,Fruchet B,da Silva J,Corbett AJ,Simoni Y,Lantz O,Rossjohn J,McCluskey J,Lesnik P,Maguin E,Lehuen A

61-6981 was used in Flow cytometry/Cell sorting to show that during obesity MAIT cells promote inflammation in both adipose tissue and ileum, leading to insulin resistance and impaired glucose and lipid metabolism.

Fri Jul 24 00:00:00 EDT 2020

Transmaternal Helicobacter pylori exposure reduces allergic airway inflammation in offspring through regulatory T cells.

The Journal of allergy and clinical immunology

Kyburz A,Fallegger A,Zhang X,Altobelli A,Artola-Boran M,Borbet T,Urban S,Paul P,Münz C,Floess S,Huehn J,Cover TL,Blaser MJ,Taube C,Müller A

61-6981 was used in Flow cytometry/Cell sorting to investigate the consequences of transmaternal exposure to H pylori in utero and/or during lactation for susceptibility to viral and bacterial infection, predisposition to allergic airway inflammation, and development of immune cell populations in the lungs and lymphoid organs.

Mon Apr 01 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6981-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation.

Immunity

Emgård J,Kammoun H,García-Cassani B,Chesné J,Parigi SM,Jacob JM,Cheng HW,Evren E,Das S,Czarnewski P,Sleiers N,Melo-Gonzalez F,Kvedaraite E,Svensson M,Scandella E,Hepworth MR,Huber S,Ludewig B,Peduto L,Villablanca EJ,Veiga-Fernandes H,Pereira JP,Flavell RA,Willinger T

61-6981 was used in Flow cytometry/Cell sorting to show that GPR183 promotes lymphoid organ development and indicate that oxysterol-GPR183-dependent positioning within tissues controls ILC3 activity and intestinal homeostasis.

Tue Jan 16 00:00:00 EST 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6981-82) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Leptin receptor signaling in T cells is required for Th17 differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Reis BS,Lee K,Fanok MH,Mascaraque C,Amoury M,Cohn LB,Rogoz A,Dallner OS,Moraes-Vieira PM,Domingos AI,Mucida D

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6981-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity.

Nature medicine

Kim HY,Lee HJ,Chang YJ,Pichavant M,Shore SA,Fitzgerald KA,Iwakura Y,Israel E,Bolger K,Faul J,DeKruyff RH,Umetsu DT

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6981-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Nuclear orphan receptor NR2F6 directly antagonizes NFAT and RORγt binding to the Il17a promoter.

Journal of autoimmunity

Hermann-Kleiter N,Meisel M,Fresser F,Thuille N,Müller M,Roth L,Katopodis A,Baier G

Published figure using ROR gamma (t) monoclonal antibody (Product # 61-6981-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

RORgammat and commensal microflora are required for the differentiation of mucosal interleukin 22-producing NKp46+ cells.

Nature immunology

Sanos SL,Bui VL,Mortha A,Oberle K,Heners C,Johner C,Diefenbach A

Thu Jan 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: MGC129539; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORgt; RP11-98D18.11-001; Thymus orphan receptor; TOR

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Gene Aliases: Nr1f3; Rorc; Rorg; RORgamma; Thor; TOR

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UniProt ID: (Mouse) P51450

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Entrez Gene ID: (Mouse) 19885

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Function(s)

transcription coactivator binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding protein binding oxysterol binding zinc ion binding sequence-specific DNA binding metal ion binding transcription factor activity, direct ligand regulated sequence-specific DNA binding C4 zinc finger nuclear receptor

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation xenobiotic metabolic process multicellular organism development regulation of glucose metabolic process regulation of steroid metabolic process cell differentiation interleukin-17 production circadian regulation of gene expression T cell differentiation in thymus cellular response to sterol T-helper cell differentiation positive regulation of circadian rhythm steroid hormone mediated signaling pathway regulation of gamma-delta T cell differentiation regulation of fat cell differentiation positive regulation of transcription, DNA-templated alpha-beta T cell differentiation rhythmic process lymph node development mucosal-associated lymphoid tissue development Peyer's patch development adipose tissue development regulation of transcription involved in cell fate commitment negative regulation of thymocyte apoptotic process T-helper 17 cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ROR gamma (t) Monoclonal Antibody (B2D), PE-eFluor™ 610, eBioscience™

ROR gamma (t) Antibody (61-6981-82) in Flow

Product Details

61-6981-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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