Invitrogen

TNF alpha Monoclonal Antibody (MP6-XT22), FITC, eBioscience™

TNF alpha Antibody in Flow Cytometry (Flow)

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TNF alpha Antibody (11-7321-41) in Flow

Journal of biomedical science 2023 - Tumor-secreted IFI35 protein activated CD8 T cells thought PI3K/AKT/mTOR pathway. A Representative Western blots of p-mTOR, mTOR, p-AKT, AKT, p-ERK, ERK, p-JNK, JNK, p-P38, P38, p-P65, P65, p-STAT3, STAT3, and GAPDH in CD8 T cells. CD8 T cells stimulated with anti-CD3 (1 ug/mL), anti-CD28 (1 ug/mL), and IL-2 (10 ng/mL) were treated with supernatant from IFI35 knockdown or overexpression CT26 and MC38 cel... View More

TNF alpha Antibody in Immunohistochemistry (IHC)

TNF alpha Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

11-7321-41

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 82 publications 82 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP6-XT22

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670212

Product Specific Information

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+)T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported: The MP6-XT22 antibody has been reported for use in ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: The MP6-XT22 antibody has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at test size: 5 µL (0.06 µg) per test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Tumor-secreted IFI35 promotes proliferation and cytotoxic activity of CD8⁺ T cells through PI3K/AKT/mTOR signaling pathway in colorectal cancer.

Journal of biomedical science

Li P,Zhou D,Chen D,Cheng Y,Chen Y,Lin Z,Zhang X,Huang Z,Cai J,Huang W,Lin Y,Ke H,Long J,Zou Y,Ye S,Lan P

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed Jun 28 00:00:00 EDT 2023

Conditional Knockout of Hypoxia-Inducible Factor 1-Alpha in Tumor-Infiltrating Neutrophils Protects against Pancreatic Ductal Adenocarcinoma.

International journal of molecular sciences

Sieow JL,Penny HL,Gun SY,Tan LQ,Duan K,Yeong JPS,Pang A,Lim D,Toh HC,Lim TKH,Engleman E,Rotzschke O,Ng LG,Chen J,Tan SM,Wong SC

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

Yang G,Wang J,Wang S,Zhu Y

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Sat Aug 20 00:00:00 EDT 2022

Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity.

Cancer cell

Chryplewicz A,Scotton J,Tichet M,Zomer A,Shchors K,Joyce JA,Homicsko K,Hanahan D

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Oct 10 00:00:00 EDT 2022

Airway Prevotella promote TLR2-dependent neutrophil activation and rapid clearance of Streptococcus pneumoniae from the lung.

Nature communications

Horn KJ,Schopper MA,Drigot ZG,Clark SE

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Jun 09 00:00:00 EDT 2022

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8⁺ T lymphocytes.

Nature communications

Tan TCJ,Kelly V,Zou X,Wright D,Ly T,Zamoyska R

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Sat Dec 17 00:00:00 EST 2022

Intranasal delivery of a small-molecule ErbB inhibitor promotes recovery from acute and late-stage CNS inflammation.

JCI insight

Linnerbauer M,Lößlein L,Vandrey O,Tsaktanis T,Beer A,Naumann UJ,Panier F,Beyer T,Nirschl L,Kuramatsu JB,Winkler J,Quintana FJ,Rothhammer V

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Interleukin-37 protects against acinar cell pyroptosis in acute pancreatitis.

JCI insight

Ma N,Yuan C,Shi J,Zhu Q,Liu Y,Ma X,Li B,Gong W,Xue J,Lu G,Li W,Li J

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Tue Nov 08 00:00:00 EST 2022

Circular RNA cancer vaccines drive immunity in hard-to-treat malignancies.

Theranostics

Li H,Peng K,Yang K,Ma W,Qi S,Yu X,He J,Lin X,Yu G

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2022

Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

Zou Y,Wang X,Xu J,Wang S,Li S,Zhu Y,Wang J

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Oct 31 00:00:00 EDT 2022

CXCL10 chemokine regulates heterogeneity of the CD8⁺ T cell response and viral set point during chronic infection.

Immunity

Ozga AJ,Chow MT,Lopes ME,Servis RL,Di Pilato M,Dehio P,Lian J,Mempel TR,Luster AD

11-7321-41 was used in Flow cytometry/Cell sorting to find that the chemokine receptor CXCR3 is highly expressed on viral-specific stem-like CD8+ T cells and that one of its ligands, CXCL10, regulates the persistence and heterogeneity of responding CD8+ T cells in spleens of mice chronically infected with lymphocytic choriomeningitis virus.

Tue Jan 11 00:00:00 EST 2022

COVID-19 treatments and pathogenesis including anosmia in K18-hACE2 mice.

Nature

Zheng J,Wong LR,Li K,Verma AK,Ortiz ME,Wohlford-Lenane C,Leidinger MR,Knudson CM,Meyerholz DK,McCray PB,Perlman S

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

The ubiquitin ligase MDM2 sustains STAT5 stability to control T cell-mediated antitumor immunity.

Nature immunology

Zhou J,Kryczek I,Li S,Li X,Aguilar A,Wei S,Grove S,Vatan L,Yu J,Yan Y,Liao P,Lin H,Li J,Li G,Du W,Wang W,Lang X,Wang W,Wang S,Zou W

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

Zhuang Z,Lai X,Sun J,Chen Z,Zhang Z,Dai J,Liu D,Li Y,Li F,Wang Y,Zhu A,Wang J,Yang W,Huang J,Li X,Hu L,Wen L,Zhuo J,Zhang Y,Chen D,Li S,Huang S,Shi Y,Zheng K,Zhong N,Zhao J,Zhou D,Zhao J

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

11-7321-41 was used in Flow Cytometry to test the impact of pre-erythrocytic malaria immunogenicity on the immune system response.

Fri Apr 09 00:00:00 EDT 2021

Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12.

Molecular therapy : the journal of the American Society of Gene Therapy

Teng Y,Xu F,Zhang X,Mu J,Sayed M,Hu X,Lei C,Sriwastva M,Kumar A,Sundaram K,Zhang L,Park JW,Chen SY,Zhang S,Yan J,Merchant ML,Zhang X,McClain CJ,Wolfe JK,Adcock RS,Chung D,Palmer KE,Zhang HG

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Prevention of EAE by tolerogenic vaccination with PEGylated antigenic peptides.

Therapeutic advances in chronic disease

Pfeil J,Simonetti M,Lauer U,von Thülen B,Durek P,Poulsen C,Pawlowska J,Kröger M,Krähmer R,Leenders F,Hoffmann U,Hamann A

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Tue Nov 07 00:00:00 EST 2023

M2 macrophage-derived extracellular vesicles facilitate CD8+T cell exhaustion in hepatocellular carcinoma via the miR-21-5p/YOD1/YAP/β-catenin pathway.

Cell death discovery

Pu J,Xu Z,Nian J,Fang Q,Yang M,Huang Y,Li W,Ge B,Wang J,Wei H

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

T cell-derived tumor necrosis factor induces cytotoxicity by activating RIPK1-dependent target cell death.

JCI insight

Chun N,Ang RL,Chan M,Fairchild RL,Baldwin WM,Horwitz JK,Gelles JD,Chipuk JE,Kelliher MA,Pavlov VI,Li Y,Homann D,Heeger PS,Ting AT

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed Dec 22 00:00:00 EST 2021

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed Aug 11 00:00:00 EDT 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Mechanisms that allow vaccination against an oncolytic vesicular stomatitis virus-encoded transgene to enhance safety without abrogating oncolysis.

Scientific reports

AuYeung AWK,Mould RC,Stegelmeier AA,van Vloten JP,Karimi K,Woods JP,Petrik JJ,Wood GA,Bridle BW

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

The RNase MCPIP3 promotes skin inflammation by orchestrating myeloid cytokine response.

Nature communications

Liu B,Huang J,Ashraf A,Rahaman O,Lou J,Wang L,Cai P,Wen J,Anwaar S,Liu X,Ni H,Ganguly D,Zhao J,Yang CY

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Jul 02 00:00:00 EDT 2021

DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization.

Cell death & disease

Liu L,Wu Y,Wang B,Jiang Y,Lin L,Li X,Yang S

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon May 17 00:00:00 EDT 2021

GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure.

Cell death & disease

Yu H,Yang W,Huang J,Miao X,Wang B,Ren X,Gu Y,Wang Q,Ding X,Guo X,Qian F,Zhang Y,Xu H,Zheng L,Jin M

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Dec 16 00:00:00 EST 2021

Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi.

Pathogens (Basel, Switzerland)

Medina-Buelvas DM,Rodríguez-Sosa M,Vega L

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Sat Nov 06 00:00:00 EDT 2021

Rate of Freeze Impacts the Survival and Immune Responses Post Cryoablation of Melanoma.

Frontiers in immunology

Yakkala C,Dagher J,Sempoux C,Chiang CL,Denys A,Kandalaft LE,Koppolu B,Duran R

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Dec 13 00:00:00 EST 2021

Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2.

BioMed research international

Zhao K,Tian Y,Wang J,Chen C,Pan B,Yan Z,Zhu S,Xu K

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Sep 10 00:00:00 EDT 2021

Fgr contributes to hemorrhage-induced thalamic pain by activating NF-κB/ERK1/2 pathways.

JCI insight

Huang T,Fu G,Gao J,Zhang Y,Cai W,Wu S,Jia S,Xia S,Bachmann T,Bekker A,Tao YX

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Immunohistochemistry

Thu Oct 15 00:00:00 EDT 2020

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response.

Journal of neuroinflammation

Lee S,Kim S,Kang HY,Lim HR,Kwon Y,Jo M,Jeon YM,Kim SR,Kim K,Ha CM,Lee S,Kim HJ

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Neutralization

Wed Oct 14 00:00:00 EDT 2020

Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity.

Nature communications

Toubal A,Kiaf B,Beaudoin L,Cagninacci L,Rhimi M,Fruchet B,da Silva J,Corbett AJ,Simoni Y,Lantz O,Rossjohn J,McCluskey J,Lesnik P,Maguin E,Lehuen A

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Jul 24 00:00:00 EDT 2020

Dissecting the phenotypic and functional heterogeneity of mouse inflammatory osteoclasts by the expression of Cx3cr1.

eLife

Madel MB,Ibáñez L,Ciucci T,Halper J,Rouleau M,Boutin A,Hue C,Duroux-Richard I,Apparailly F,Garchon HJ,Wakkach A,Blin-Wakkach C

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed May 13 00:00:00 EDT 2020

Tolerogenic Immunomodulation by PEGylated Antigenic Peptides.

Frontiers in immunology

Pfeil J,Simonetti M,Lauer U,Volkmer R,von Thülen B,Durek P,Krähmer R,Leenders F,Hamann A,Hoffmann U

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Memory CD4⁺ T cells enhance B-cell responses to drifting influenza immunization.

European journal of immunology

Gage E,Van Hoeven N,Dubois Cauwelaert N,Larsen SE,Erasmus J,Orr MT,Coler RN

11-7321-41 was used in Flow Cytometry to show that MHC Class II haplotype and T-cell reactivity influences subsequent hemagglutination inhibitory antibody responses.

Fri Feb 01 00:00:00 EST 2019

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed Oct 14 00:00:00 EDT 2020

ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.

Nature communications

Cao Y,Trillo-Tinoco J,Sierra RA,Anadon C,Dai W,Mohamed E,Cen L,Costich TL,Magliocco A,Marchion D,Klar R,Michel S,Jaschinski F,Reich RR,Mehrotra S,Cubillos-Ruiz JR,Munn DH,Conejo-Garcia JR,Rodriguez PC

11-7321-41 was used in Flow Cytometry to study the intrinsic mediators that render CD8+T cells dysfunctional in the tumour microenvironment.

Wed Mar 20 00:00:00 EDT 2019

Interleukin 1 receptor (IL-1R1) activation exacerbates toxin-induced acute kidney injury.

American journal of physiology. Renal physiology

Privratsky JR,Zhang J,Lu X,Rudemiller N,Wei Q,Yu YR,Gunn MD,Crowley SD

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Targeting macrophage and microglia activation with colony stimulating factor 1 receptor inhibitor is an effective strategy to treat injury-triggered neuropathic pain.

Molecular pain

Lee S,Shi XQ,Fan A,West B,Zhang J

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Oct 22 00:00:00 EDT 2018

Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.

Nature communications

Kim DH,Park HJ,Lim S,Koo JH,Lee HG,Choi JO,Oh JH,Ha SJ,Kang MJ,Lee CM,Lee CG,Elias JA,Choi JM

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

NF-κB c-Rel Is Crucial for the Regulatory T Cell Immune Checkpoint in Cancer.

Cell

Grinberg-Bleyer Y,Oh H,Desrichard A,Bhatt DM,Caron R,Chan TA,Schmid RM,Klein U,Hayden MS,Ghosh S

11-7321-41 was used in Flow cytometry/Cell sorting to understand the mechanisms governing regulatory T cells homeostasis.

Thu Sep 07 00:00:00 EDT 2017

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Cell metabolism

Thwe PM,Pelgrom LR,Cooper R,Beauchamp S,Reisz JA,D'Alessandro A,Everts B,Amiel E

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Tue Sep 05 00:00:00 EDT 2017

A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.

The Journal of clinical investigation

Berlato C,Khan MN,Schioppa T,Thompson R,Maniati E,Montfort A,Jangani M,Canosa M,Kulbe H,Hagemann UB,Duncan AR,Fletcher L,Wilkinson RW,Powles T,Quezada SA,Balkwill FR

11-7321 was used in Flow cytometry/Cell sorting to determine the effect of CCR4 antagonism on human renal cell carcinoma.

Wed Mar 01 00:00:00 EST 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

The TLR7 agonist imiquimod induces anti-cancer effects via autophagic cell death and enhances anti-tumoral and systemic immunity during radiotherapy for melanoma.

Oncotarget

Cho JH,Lee HJ,Ko HJ,Yoon BI,Choe J,Kim KC,Hahn TW,Han JA,Choi SS,Jung YM,Lee KH,Lee YS,Jung YJ

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Tue Apr 11 00:00:00 EDT 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

CD4(+) T-cell dependence of primary CD8(+) T-cell response against vaccinia virus depends upon route of infection and viral dose.

Cellular & molecular immunology

Hu Z,Molloy MJ,Usherwood EJ

11-7321 was used in Flow cytometry/Cell sorting to investigate the role of of CD4(+) T-cell help in CD8(+) T-cell responses against viral infections.

Fri Jan 01 00:00:00 EST 2016

Antitumor adaptive immunity remains intact following inhibition of autophagy and antimalarial treatment.

The Journal of clinical investigation

Starobinets H,Ye J,Broz M,Barry K,Goldsmith J,Marsh T,Rostker F,Krummel M,Debnath J

11-7321 was used in Flow cytometry/Cell sorting to evaluate how autophagy inhibition impacts the antitumor immune response in immune-competent mouse models of melanoma and mammary cancer.

Thu Dec 01 00:00:00 EST 2016

TGF-β receptor maintains CD4 T helper cell identity during chronic viral infections.

The Journal of clinical investigation

Lewis GM,Wehrens EJ,Labarta-Bajo L,Streeck H,Zuniga EI

11-7321 was used in Flow cytometry/Cell sorting to uncover an eomesodermin-driven CD4 T cell program that is continuously suppressed by TGF-β signalling.

Mon Oct 03 00:00:00 EDT 2016

Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion.

The Journal of experimental medicine

Hu Y,Kim JH,He K,Wan Q,Kim J,Flach M,Kirchhausen T,Vortkamp A,Winau F

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Nov 14 00:00:00 EST 2016

Reprogramming the immunological microenvironment through radiation and targeting Axl.

Nature communications

Aguilera TA,Rafat M,Castellini L,Shehade H,Kariolis MS,Hui AB,Stehr H,von Eyben R,Jiang D,Ellies LG,Koong AC,Diehn M,Rankin EB,Graves EE,Giaccia AJ

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2016

Dendritic cell function and pathogen-specific T cell immunity are inhibited in mice administered levonorgestrel prior to intranasal Chlamydia trachomatis infection.

Scientific reports

Quispe Calla NE,Vicetti Miguel RD,Mei A,Fan S,Gilmore JR,Cherpes TL

11-7321 was used in Flow cytometry/Cell sorting to demonstrate that levonorgestrel suppresses dendritic cell activation and function, and inhibits formation of pathogen-specific T-cell immunity.

Mon Nov 28 00:00:00 EST 2016

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks.

Cell

Parnas O,Jovanovic M,Eisenhaure TM,Herbst RH,Dixit A,Ye CJ,Przybylski D,Platt RJ,Tirosh I,Sanjana NE,Shalem O,Satija R,Raychowdhury R,Mertins P,Carr SA,Zhang F,Hacohen N,Regev A

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

Early virus-host interactions dictate the course of a persistent infection.

PLoS pathogens

Sullivan BM,Teijaro JR,de la Torre JC,Oldstone MB

11-7321 was used in Flow cytometry/Cell sorting to investigate the very early host-virus interactions that determine the establishment of viral persistence.

Thu Jan 01 00:00:00 EST 2015

Alterations in antigen-specific naive CD4 T cell precursors after sepsis impairs their responsiveness to pathogen challenge.

Journal of immunology (Baltimore, Md. : 1950)

Cabrera-Perez J,Condotta SA,James BR,Kashem SW,Brincks EL,Rai D,Kucaba TA,Badovinac VP,Griffith TS

11-7321 was used in Flow cytometry/Cell sorting to demonstrate that the recovery of CD4 T cells from sepsis-induced lymphopenia is accompanied by alterations to the composition and function of the Ag-specific CD4 T cell repertoire.

Sun Feb 15 00:00:00 EST 2015

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

Alphavirus-based vaccines encoding nonstructural proteins of hepatitis C virus induce robust and protective T-cell responses.

Molecular therapy : the journal of the American Society of Gene Therapy

Ip PP,Boerma A,Regts J,Meijerhof T,Wilschut J,Nijman HW,Daemen T

11-7321 was used in Flow cytometry/Cell sorting to study an rSFV-based vector which induces robust T-cell activity and has a potential for the treatment of HCV infections.

Tue Apr 01 00:00:00 EDT 2014

GM-CSF but not IL-17 is critical for the development of severe interstitial lung disease in SKG mice.

Journal of immunology (Baltimore, Md. : 1950)

Shiomi A,Usui T,Ishikawa Y,Shimizu M,Murakami K,Mimori T

11-7321 was used in Flow cytometry/Cell sorting to investigate the importance of GM-CSF in the development of severe interstitial lung disease.

Tue Jul 15 00:00:00 EDT 2014

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

Hematopoietic not systemic impairment of Roquin expression accounts for intestinal inflammation in Roquin-deficient mice.

Scientific reports

Montufar-Solis D,Vigneswaran N,Nakra N,Schaefer JS,Klein JR

11-7321 was used in Flow cytometry/Cell sorting to investigate the intestinal immunopathology caused by insufficient Roquin, showing that hematopoietic, not systematic, impairment of Roquin expression accounts for pathology.

Mon May 12 00:00:00 EDT 2014

Mechanism of tumor rejection with doublets of CTLA-4, PD-1/PD-L1, or IDO blockade involves restored IL-2 production and proliferation of CD8(+) T cells directly within the tumor microenvironment.

Journal for immunotherapy of cancer

Spranger S,Koblish HK,Horton B,Scherle PA,Newton R,Gajewski TF

11-7321 was used in Flow cytometry/Cell sorting to assess the effectiveness of different immunotherapy combination treatments against the murine B16.SIY melanoma model.

Thu May 15 00:00:00 EDT 2014

Immunosuppression after sepsis: systemic inflammation and sepsis induce a loss of naïve T-cells but no enduring cell-autonomous defects in T-cell function.

PloS one

Markwart R,Condotta SA,Requardt RP,Borken F,Schubert K,Weigel C,Bauer M,Griffith TS,Förster M,Brunkhorst FM,Badovinac VP,Rubio I

11-7321 was used in Flow cytometry/Cell sorting to investigate the nature of defects of T-cell immunity in post-acute phases of systemic inflammatory response, showing an induced loss of naive T-cells but no enduring cell-autonomous defects in T-cell function.

Thu Aug 27 00:00:00 EDT 2015

Induction of broad cytotoxic T cells by protective DNA vaccination against Marburg and Ebola.

Molecular therapy : the journal of the American Society of Gene Therapy

Shedlock DJ,Aviles J,Talbott KT,Wong G,Wu SJ,Villarreal DO,Myles DJ,Croyle MA,Yan J,Kobinger GP,Weiner DB

11-7321 was used in Flow cytometry/Cell sorting to investigate the efficacy of cytotoxic T cell induction by a novel polyvalent-filovirus DNA vaccine against Marburg and Ebola.

Mon Jul 01 00:00:00 EDT 2013

Markers of T cell infiltration and function associate with favorable outcome in vascularized high-grade serous ovarian carcinoma.

PloS one

Townsend KN,Spowart JE,Huwait H,Eshragh S,West NR,Elrick MA,Kalloger SE,Anglesio M,Watson PH,Huntsman DG,Lum JJ

11-7321 was used in Flow cytometry/Cell sorting to determine whether tumour vasculature influences the functional capacity of tumour infiltrating lymphocytes, and correlate this with survival in ovarian cancer patients.

Tue Mar 03 00:00:00 EST 2015

Mixed vector immunization with recombinant adenovirus and MVA can improve vaccine efficacy while decreasing antivector immunity.

Molecular therapy : the journal of the American Society of Gene Therapy

Reyes-Sandoval A,Rollier CS,Milicic A,Bauza K,Cottingham MG,Tang CK,Dicks MD,Wang D,Longley RJ,Wyllie DH,Hill AV

11-7321 was used in Immunocytochemistry to investigate whether the two vaccine components adenovirus and modified vaccinia virus Ankara can be co-injected to enhance protection against malaria.

Wed Aug 01 00:00:00 EDT 2012

ORFV: a novel oncolytic and immune stimulating parapoxvirus therapeutic.

Molecular therapy : the journal of the American Society of Gene Therapy

Rintoul JL,Lemay CG,Tai LH,Stanford MM,Falls TJ,de Souza CT,Bridle BW,Daneshmand M,Ohashi PS,Wan Y,Lichty BD,Mercer AA,Auer RC,Atkins HL,Bell JC

11-7321 was used in Flow cytometry/Cell sorting to investigate the use of Parapoxvirus ovis for the treatment of cancer.

Fri Jun 01 00:00:00 EDT 2012

Therapeutic blockade of transforming growth factor beta fails to promote clearance of a persistent viral infection.

Journal of virology

Garidou L,Heydari S,Gossa S,McGavern DB

11-7321 was used in Flow cytometry/Cell sorting to assess transforming growth factor beta inhibition as a viable approach to control a persistent viral infection.

Sun Jul 01 00:00:00 EDT 2012

TLR9 and MyD88 are crucial for the development of protective immunity to malaria.

Journal of immunology (Baltimore, Md. : 1950)

Gowda NM,Wu X,Gowda DC

11-7321 was used in an ELISA assay to investigate TLR9 and MyD88 regulation of pro/anti-inflammatory cytokines in mice infected with Plasmodium yoelii.

Tue May 15 00:00:00 EDT 2012

Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.

Vaccine

Goicochea MA,Zapata JC,Bryant J,Davis H,Salvato MS,Lukashevich IS

11-7321 was used in Flow cytometry/Cell sorting to determine the usefulness of a CBA/J-ML29 mouse model as a tool for evaluating the efficacy of vaccine candidates against LASV.

Tue Feb 14 00:00:00 EST 2012

Failure to recruit anti-inflammatory CD103+ dendritic cells and a diminished CD4+ Foxp3+ regulatory T cell pool in mice that display excessive lung inflammation and increased susceptibility to Mycobacterium tuberculosis.

Infection and immunity

Leepiyasakulchai C,Ignatowicz L,Pawlowski A,Källenius G,Sköld M

11-7321 was used in Immunocytochemistry to increase our understanding of mechanisms that may regulate the host immune response in M. tuberculosis-infected lungs.

Thu Mar 01 00:00:00 EST 2012

Tissue specific deletion of inhibitor of kappa B kinase 2 with OX40-Cre reveals the unanticipated expression from the OX40 locus in skin epidermis.

PloS one

Cornish GH,Tung SL,Marshall D,Ley S,Seddon BP

Published figure using TNF alpha monoclonal antibody (Product # 11-7321-41) in Immunofluorescence

Fri Jun 29 00:00:00 EDT 2012

Endostatin gene therapy stimulates upregulation of ICAM-1 and VCAM-1 in a metastatic renal cell carcinoma model.

Cancer gene therapy

Chaves KC,Peron JP,Chammas R,Turaça LT,Pesquero JB,Braga MS,Foguer K,Schor N,Bellini MH

11-7321-41 was used in Flow cytometry/Cell sorting to demonstrate an antitumor inflammatory effect of Endostatin (ES) in an mRCC model, and this effect is mediated by an increase in ICAM-1 and VCAM-1 expression in tumor-associated endothelial cells.

Wed Aug 01 00:00:00 EDT 2012

A role for IL-27 in limiting T regulatory cell populations.

Journal of immunology (Baltimore, Md. : 1950)

Wojno ED,Hosken N,Stumhofer JS,O'Hara AC,Mauldin E,Fang Q,Turka LA,Levin SD,Hunter CA

11-7321 was used in Flow cytometry/Cell sorting to show that mice that overexpress IL-27 had decreased Treg frequencies and developed spontaneous inflammation.

Fri Jul 01 00:00:00 EDT 2011

Autoreactive Tbet-positive CD4 T cells develop independent of classic Th1 cytokine signaling during experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Yeh WI,McWilliams IL,Harrington LE

11-7321 was used in Flow cytometry/Cell sorting to study changes in expression of Tbet by CD4 T cells from distinct areas of the body following the onset of experimental autoimmune encephalomyelitis.

Tue Nov 15 00:00:00 EST 2011

The magnitude of the T cell response to a clinically significant dose of influenza virus is regulated by TRAIL.

Journal of immunology (Baltimore, Md. : 1950)

Brincks EL,Gurung P,Langlois RA,Hemann EA,Legge KL,Griffith TS

11-7321 was used in Flow cytometry/Cell sorting to study how TRAIL affects the T cell response and ultimately influences the outcome of viral infection.

Tue Nov 01 00:00:00 EDT 2011

Preferential replication of vaccinia virus in the ovaries is independent of immune regulation through IL-10 and TGF-β.

Viral immunology

Zhao Y,Adams YF,Croft M

11-7321 was used in Flow cytometry/Cell sorting to study how the preferential replication of VACV in the ovaries may not be dependent on immune suppressive mechanisms in this tissue.

Sat Oct 01 00:00:00 EDT 2011

NK cells delay allograft rejection in lymphopenic hosts by downregulating the homeostatic proliferation of CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Zecher D,Li Q,Oberbarnscheidt MH,Demetris AJ,Shlomchik WD,Rothstein DM,Lakkis FG

11-7321 was used in Flow cytometry/Cell sorting to demonstrate that NK cells downregulate homeostatic CD8+ T-cell proliferation in lymphopenic environments by competing for IL-15.

Tue Jun 15 00:00:00 EDT 2010

Ability of IDO to attenuate liver injury in alpha-galactosylceramide-induced hepatitis model.

Journal of immunology (Baltimore, Md. : 1950)

Ito H,Hoshi M,Ohtaki H,Taguchi A,Ando K,Ishikawa T,Osawa Y,Hara A,Moriwaki H,Saito K,Seishima M

11-7321 was used in Flow cytometry/Cell sorting to study the immunological effects of IDO in α-galactosylceramide (α-GalCer)-induced hepatitis.

Fri Oct 15 00:00:00 EDT 2010

Ability of IDO to attenuate liver injury in alpha-galactosylceramide-induced hepatitis model.

Journal of immunology (Baltimore, Md. : 1950)

Ito H,Hoshi M,Ohtaki H,Taguchi A,Ando K,Ishikawa T,Osawa Y,Hara A,Moriwaki H,Saito K,Seishima M

11-7321 was used in Flow cytometry/Cell sorting to study the immunological effects of IDO in α-galactosylceramide (α-GalCer)-induced hepatitis.

Fri Oct 15 00:00:00 EDT 2010

Therapeutic memory T cells require costimulation for effective clearance of a persistent viral infection.

Journal of virology

Garidou L,Heydari S,Truong P,Brooks DG,McGavern DB

11-7321 was used in Immunofluorescence to reveal that costimulatory pathways can be targeted to modulate the magnitude of an adoptive immunotherapeutic regimen.

Tue Sep 01 00:00:00 EDT 2009

Increased myeloid cell responses to M-CSF and RANKL cause bone loss and inflammation in SH3BP2 "cherubism" mice.

Cell

Ueki Y,Lin CY,Senoo M,Ebihara T,Agata N,Onji M,Saheki Y,Kawai T,Mukherjee PM,Reichenberger E,Olsen BR

11-7321 was used in Flow cytometry/Cell sorting to demonstrate that Sh3bp2 "cherubism'' mice exhibit trabecular bone loss, TNF-α-dependent systemic inflammation, and cortical bone erosion.

Fri Jan 12 00:00:00 EST 2007

The surprising kinetics of the T cell response to live antigenic cells.

Journal of immunology (Baltimore, Md. : 1950)

Tyznik AJ,Bevan MJ

11-7321 was used in Flow cytometry/Cell sorting to demonstrate dramatic differences in host T cell responses to noninflammatory Ags compared with responses to pathogens.

Mon Oct 15 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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Gene Aliases: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2

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UniProt ID: (Mouse) P06804

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Entrez Gene ID: (Mouse) 21926

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Function(s)

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

Process(es)

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TNF alpha Monoclonal Antibody (MP6-XT22), FITC, eBioscience™

TNF alpha Antibody (11-7321-41) in Flow

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11-7321-41

Immunohistochemistry (IHC)

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