Invitrogen

TNF alpha Monoclonal Antibody (MP6-XT22), PE-Cyanine7, eBioscience™

TNF alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 64

TNF alpha Antibody (25-7321-82) in Flow

Mouse splenocytes were unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) (right) for 5 hours. Cells were fixed and permeabilized and stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0041-82) and 0.06 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) or 0.06 µg of Anti-Mouse TNF alpha PE-Cyanine7. Viable cells as determined Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

25-7321-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

- -

Flow Cytometry (Flow)

0.125 µg/test

View 68 publications 68 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP6-XT22

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11042728

Product Specific Information

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported: This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MP6-XT22 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Tumor-secreted IFI35 promotes proliferation and cytotoxic activity of CD8⁺ T cells through PI3K/AKT/mTOR signaling pathway in colorectal cancer.

Journal of biomedical science

Li P,Zhou D,Chen D,Cheng Y,Chen Y,Lin Z,Zhang X,Huang Z,Cai J,Huang W,Lin Y,Ke H,Long J,Zou Y,Ye S,Lan P

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed Jun 28 00:00:00 EDT 2023

Conditional Knockout of Hypoxia-Inducible Factor 1-Alpha in Tumor-Infiltrating Neutrophils Protects against Pancreatic Ductal Adenocarcinoma.

International journal of molecular sciences

Sieow JL,Penny HL,Gun SY,Tan LQ,Duan K,Yeong JPS,Pang A,Lim D,Toh HC,Lim TKH,Engleman E,Rotzschke O,Ng LG,Chen J,Tan SM,Wong SC

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2023

Fc-γR-dependent antibody effector functions are required for vaccine-mediated protection against antigen-shifted variants of SARS-CoV-2.

Nature microbiology

Mackin SR,Desai P,Whitener BM,Karl CE,Liu M,Baric RS,Edwards DK,Chicz TM,McNamara RP,Alter G,Diamond MS

25-7321-82 was used in Flow cytometry/Cell sorting to suggest that Fc-FcγR engagement and alveolar macrophages are required for vaccine-induced antibody-mediated protection against infection by antigenically changed SARS-CoV-2 variants, including Omicron strains.

Sat Apr 01 00:00:00 EDT 2023

Forsythiaside A Improves the Inhibitory Efficiency of Recombinant Protein Vaccines against Bovine Viral Diarrhea Virus Infection.

International journal of molecular sciences

Yang G,Wang J,Wang S,Zhu Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Sat Aug 20 00:00:00 EDT 2022

Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity.

Cancer cell

Chryplewicz A,Scotton J,Tichet M,Zomer A,Shchors K,Joyce JA,Homicsko K,Hanahan D

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon Oct 10 00:00:00 EDT 2022

Airway Prevotella promote TLR2-dependent neutrophil activation and rapid clearance of Streptococcus pneumoniae from the lung.

Nature communications

Horn KJ,Schopper MA,Drigot ZG,Clark SE

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Thu Jun 09 00:00:00 EDT 2022

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8⁺ T lymphocytes.

Nature communications

Tan TCJ,Kelly V,Zou X,Wright D,Ly T,Zamoyska R

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Sat Dec 17 00:00:00 EST 2022

Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8⁺ T lymphocytes.

Nature communications

Tan TCJ,Kelly V,Zou X,Wright D,Ly T,Zamoyska R

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Sat Dec 17 00:00:00 EST 2022

Intranasal delivery of a small-molecule ErbB inhibitor promotes recovery from acute and late-stage CNS inflammation.

JCI insight

Linnerbauer M,Lößlein L,Vandrey O,Tsaktanis T,Beer A,Naumann UJ,Panier F,Beyer T,Nirschl L,Kuramatsu JB,Winkler J,Quintana FJ,Rothhammer V

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Interleukin-37 protects against acinar cell pyroptosis in acute pancreatitis.

JCI insight

Ma N,Yuan C,Shi J,Zhu Q,Liu Y,Ma X,Li B,Gong W,Xue J,Lu G,Li W,Li J

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Tue Nov 08 00:00:00 EST 2022

Circular RNA cancer vaccines drive immunity in hard-to-treat malignancies.

Theranostics

Li H,Peng K,Yang K,Ma W,Qi S,Yu X,He J,Lin X,Yu G

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Thu Sep 29 00:00:00 EDT 2022

Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

Zou Y,Wang X,Xu J,Wang S,Li S,Zhu Y,Wang J

25-7321-82 was used in Flow cytometry/Cell sorting to reveal that Z. morio hemolymph alleviates E. coli-induced mastitis via lessening the inflammatory response by regulating the NLRP3 and ATG5/ATG16L1 signaling pathway, as well as repairing the blood-milk barrier.

Mon Oct 31 00:00:00 EDT 2022

Z. morio Hemolymph Relieves E. coli-Induced Mastitis by Inhibiting Inflammatory Response and Repairing the Blood-Milk Barrier.

International journal of molecular sciences

Zou Y,Wang X,Xu J,Wang S,Li S,Zhu Y,Wang J

Mon Oct 31 00:00:00 EDT 2022

Neoantigen cancer vaccine augments anti-CTLA-4 efficacy.

NPJ vaccines

Salvatori E,Lione L,Compagnone M,Pinto E,Conforti A,Ciliberto G,Aurisicchio L,Palombo F

25-7321-82 was used in Flow Cytometry to support the use of NCV delivered by DNA-EP with αCTLA-4 and suggest a new combined therapy for clinical testing.

Wed Feb 02 00:00:00 EST 2022

Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury.

Liver international : official journal of the International Association for the Study of the Liver

Li Y,Li TY,Qi Q,Zhang MT,Tong MX,Su PJ,Zhang ZB

25-7321-82 was used in Flow cytometry/Cell sorting to show that poliovirus receptor contributes to BA pathogenesis by regulating NK cell-mediated bile duct injury; PVR has the value as a biomarker of BA.

Thu Dec 01 00:00:00 EST 2022

COVID-19 treatments and pathogenesis including anosmia in K18-hACE2 mice.

Nature

Zheng J,Wong LR,Li K,Verma AK,Ortiz ME,Wohlford-Lenane C,Leidinger MR,Knudson CM,Meyerholz DK,McCray PB,Perlman S

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

The ubiquitin ligase MDM2 sustains STAT5 stability to control T cell-mediated antitumor immunity.

Nature immunology

Zhou J,Kryczek I,Li S,Li X,Aguilar A,Wei S,Grove S,Vatan L,Yu J,Yan Y,Liao P,Lin H,Li J,Li G,Du W,Wang W,Lang X,Wang W,Wang S,Zou W

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

Zhuang Z,Lai X,Sun J,Chen Z,Zhang Z,Dai J,Liu D,Li Y,Li F,Wang Y,Zhu A,Wang J,Yang W,Huang J,Li X,Hu L,Wen L,Zhuo J,Zhang Y,Chen D,Li S,Huang S,Shi Y,Zheng K,Zhong N,Zhao J,Zhou D,Zhao J

25-7321 was used in Flow cytometry/Cell sorting to demonstrate cross-reactive T cell responses between SARS-CoV and SARS-CoV-2, but not MERS-CoV, in mice.

Mon Apr 05 00:00:00 EDT 2021

Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

Zhuang Z,Lai X,Sun J,Chen Z,Zhang Z,Dai J,Liu D,Li Y,Li F,Wang Y,Zhu A,Wang J,Yang W,Huang J,Li X,Hu L,Wen L,Zhuo J,Zhang Y,Chen D,Li S,Huang S,Shi Y,Zheng K,Zhong N,Zhao J,Zhou D,Zhao J

25-7321 was used in Flow cytometry/Cell sorting to demonstrate cross-reactive T cell responses between SARS-CoV and SARS-CoV-2, but not MERS-CoV, in mice.

Mon Apr 05 00:00:00 EDT 2021

Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12.

Molecular therapy : the journal of the American Society of Gene Therapy

Teng Y,Xu F,Zhang X,Mu J,Sayed M,Hu X,Lei C,Sriwastva M,Kumar A,Sundaram K,Zhang L,Park JW,Chen SY,Zhang S,Yan J,Merchant ML,Zhang X,McClain CJ,Wolfe JK,Adcock RS,Chung D,Palmer KE,Zhang HG

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Prevention of EAE by tolerogenic vaccination with PEGylated antigenic peptides.

Therapeutic advances in chronic disease

Pfeil J,Simonetti M,Lauer U,von Thülen B,Durek P,Poulsen C,Pawlowska J,Kröger M,Krähmer R,Leenders F,Hoffmann U,Hamann A

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Tue Nov 07 00:00:00 EST 2023

M2 macrophage-derived extracellular vesicles facilitate CD8+T cell exhaustion in hepatocellular carcinoma via the miR-21-5p/YOD1/YAP/β-catenin pathway.

Cell death discovery

Pu J,Xu Z,Nian J,Fang Q,Yang M,Huang Y,Li W,Ge B,Wang J,Wei H

25-7321-82 was used in Flow Cytometry to demonstrate the involvement of M2 macrophage-derived extracellular vesicles in hepatocellular carcinoma-associated T cell exhaustion.

Fri Jul 16 00:00:00 EDT 2021

M2 macrophage-derived extracellular vesicles facilitate CD8+T cell exhaustion in hepatocellular carcinoma via the miR-21-5p/YOD1/YAP/β-catenin pathway.

Cell death discovery

Pu J,Xu Z,Nian J,Fang Q,Yang M,Huang Y,Li W,Ge B,Wang J,Wei H

25-7321-82 was used in Flow Cytometry to demonstrate the involvement of M2 macrophage-derived extracellular vesicles in hepatocellular carcinoma-associated T cell exhaustion.

Fri Jul 16 00:00:00 EDT 2021

T cell-derived tumor necrosis factor induces cytotoxicity by activating RIPK1-dependent target cell death.

JCI insight

Chun N,Ang RL,Chan M,Fairchild RL,Baldwin WM,Horwitz JK,Gelles JD,Chipuk JE,Kelliher MA,Pavlov VI,Li Y,Homann D,Heeger PS,Ting AT

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed Dec 22 00:00:00 EST 2021

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed Aug 11 00:00:00 EDT 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Mechanisms that allow vaccination against an oncolytic vesicular stomatitis virus-encoded transgene to enhance safety without abrogating oncolysis.

Scientific reports

AuYeung AWK,Mould RC,Stegelmeier AA,van Vloten JP,Karimi K,Woods JP,Petrik JJ,Wood GA,Bridle BW

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Tue Jul 27 00:00:00 EDT 2021

The RNase MCPIP3 promotes skin inflammation by orchestrating myeloid cytokine response.

Nature communications

Liu B,Huang J,Ashraf A,Rahaman O,Lou J,Wang L,Cai P,Wen J,Anwaar S,Liu X,Ni H,Ganguly D,Zhao J,Yang CY

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Jul 02 00:00:00 EDT 2021

DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization.

Cell death & disease

Liu L,Wu Y,Wang B,Jiang Y,Lin L,Li X,Yang S

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon May 17 00:00:00 EDT 2021

GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure.

Cell death & disease

Yu H,Yang W,Huang J,Miao X,Wang B,Ren X,Gu Y,Wang Q,Ding X,Guo X,Qian F,Zhang Y,Xu H,Zheng L,Jin M

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Thu Dec 16 00:00:00 EST 2021

Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi.

Pathogens (Basel, Switzerland)

Medina-Buelvas DM,Rodríguez-Sosa M,Vega L

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Sat Nov 06 00:00:00 EDT 2021

Antitumor efficacy of a neoantigen cancer vaccine delivered by electroporation is influenced by microbiota composition.

Oncoimmunology

Lione L,Salvatori E,Petrazzuolo A,Massacci A,Maggio R,Confroti A,Compagnone M,Aurisicchio L,Ciliberto G,Palombo F

25-7321-82 was used in Flow cytometry/Cell sorting to find that decreased microbiota diversity induced by prolonged antibiotic (ATB) treatment is associated with higher intratumor specific immune responses and consequently to a better antitumor effect induced by neoantigen cancer vaccines (NCVs) delivered by DNA-EP.

Tue Mar 23 00:00:00 EDT 2021

Rate of Freeze Impacts the Survival and Immune Responses Post Cryoablation of Melanoma.

Frontiers in immunology

Yakkala C,Dagher J,Sempoux C,Chiang CL,Denys A,Kandalaft LE,Koppolu B,Duran R

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon Dec 13 00:00:00 EST 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nature communications

Sun Y,Lu D,Yin Y,Song J,Liu Y,Hao W,Qi F,Zhang G,Zhang X,Liu L,Lin Z,Liang H,Zhao X,Jin Y,Yin Y

25-7321-82 was used in Flow Cytometry to propose that PTENα protects tumor from T cell killing and thus PTENα is a potential target in antitumor immunotherapy.

Thu Aug 26 00:00:00 EDT 2021

Metabolic requirements of NK cells during the acute response against retroviral infection.

Nature communications

Littwitz-Salomon E,Moreira D,Frost JN,Choi C,Liou KT,Ahern DK,O'Shaughnessy S,Wagner B,Biron CA,Drakesmith H,Dittmer U,Finlay DK

25-7321-82 was used in Flow cytometry/Cell sorting to show that following infection natural killer cells increase nutrient uptake, including amino acids and iron, and reprogram their metabolic machinery by increasing glycolysis and mitochondrial metabolism.

Fri Sep 10 00:00:00 EDT 2021

A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by T_reg cells.

Nature

Nasrallah R,Imianowski CJ,Bossini-Castillo L,Grant FM,Dogan M,Placek L,Kozhaya L,Kuo P,Sadiyah F,Whiteside SK,Mumbach MR,Glinos D,Vardaka P,Whyte CE,Lozano T,Fujita T,Fujii H,Liston A,Andrews S,Cozzani A,Yang J,Mitra S,Lugli E,Chang HY,Unutmaz D,Trynka G,Roychoudhuri R

25-7321 was used in Flow cytometry/Cell sorting to provide a mechanistic basis for association of the 11q13.5 risk locus with immune-mediated diseases and identify GARP as a potential target in their therapy.

Wed Jul 01 00:00:00 EDT 2020

Developmental Relationships of Four Exhausted CD8⁺ T Cell Subsets Reveals Underlying Transcriptional and Epigenetic Landscape Control Mechanisms.

Immunity

Beltra JC,Manne S,Abdel-Hakeem MS,Kurachi M,Giles JR,Chen Z,Casella V,Ngiow SF,Khan O,Huang YJ,Yan P,Nzingha K,Xu W,Amaravadi RK,Xu X,Karakousis GC,Mitchell TC,Schuchter LM,Huang AC,Wherry EJ

25-7321-82 was used in Flow cytometry/Cell sorting to reveal a four-stage developmental hierarchy for Tex cells and define the molecular, transcriptional, and epigenetic mechanisms that could provide opportunities to improve cancer immunotherapy.

Tue May 19 00:00:00 EDT 2020

Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2.

BioMed research international

Zhao K,Tian Y,Wang J,Chen C,Pan B,Yan Z,Zhu S,Xu K

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Sep 10 00:00:00 EDT 2021

Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells and Provide Protection from Plasmodium Challenge in a Mouse Model.

Vaccines

Reeder SM,Reuschel EL,Bah MA,Yun K,Tursi NJ,Kim KY,Chu J,Zaidi FI,Yilmaz I,Hart RJ,Perrin B,Xu Z,Humeau L,Weiner DB,Aly ASI

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2020

Deletion of immune evasion genes provides an effective vaccine design for tumor-associated herpesviruses.

NPJ vaccines

Brar G,Farhat NA,Sukhina A,Lam AK,Kim YH,Hsu T,Tong L,Lin WW,Ware CF,Blackman MA,Sun R,Wu TT

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Thu Nov 05 00:00:00 EST 2020

Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity.

Nature communications

Toubal A,Kiaf B,Beaudoin L,Cagninacci L,Rhimi M,Fruchet B,da Silva J,Corbett AJ,Simoni Y,Lantz O,Rossjohn J,McCluskey J,Lesnik P,Maguin E,Lehuen A

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Jul 24 00:00:00 EDT 2020

Dissecting the phenotypic and functional heterogeneity of mouse inflammatory osteoclasts by the expression of Cx3cr1.

eLife

Madel MB,Ibáñez L,Ciucci T,Halper J,Rouleau M,Boutin A,Hue C,Duroux-Richard I,Apparailly F,Garchon HJ,Wakkach A,Blin-Wakkach C

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed May 13 00:00:00 EDT 2020

High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways.

Cell reports

Covarrubias S,Vollmers AC,Capili A,Boettcher M,Shulkin A,Correa MR,Halasz H,Robinson EK,O'Briain L,Vollmers C,Blau J,Katzman S,McManus MT,Carpenter S

25-7321-82 was used in Flow Cytometry to unravel complexities of the TNF signaling cascade, showing that it can function in an autocrine manner in macrophages to negatively regulate the pathway.

Tue Dec 29 00:00:00 EST 2020

Modulation of Vaccine-Induced HIV-1-Specific Immune Responses by Co-Electroporation of PD-L1 Encoding DNA.

Vaccines

Tannig P,Peter AS,Lapuente D,Klessing S,Damm D,Tenbusch M,Überla K,Temchura V

25-7321-82 was used in Flow Cytometry to indicate that co-electroporation of soluble checkpoint ectodomains together with DNA-based vaccines has modulatory effects on vaccine-induced immune responses that could improve vaccine efficacies.

Tue Jan 14 00:00:00 EST 2020

Tolerogenic Immunomodulation by PEGylated Antigenic Peptides.

Frontiers in immunology

Pfeil J,Simonetti M,Lauer U,Volkmer R,von Thülen B,Durek P,Krähmer R,Leenders F,Hamann A,Hoffmann U

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed Oct 14 00:00:00 EDT 2020

Aging alters the immunological response to ischemic stroke.

Acta neuropathologica

Ritzel RM,Lai YJ,Crapser JD,Patel AR,Schrecengost A,Grenier JM,Mancini NS,Patrizz A,Jellison ER,Morales-Scheihing D,Venna VR,Kofler JK,Liu F,Verma R,McCullough LD

25-7321 was used in Flow Cytometry to study the marked differences in the composition of circulating and infiltrating leukocytes recruited to the ischaemic brain of old male mice after stroke compared to young male mice.

Sun Jul 01 00:00:00 EDT 2018

Interleukin 1 receptor (IL-1R1) activation exacerbates toxin-induced acute kidney injury.

American journal of physiology. Renal physiology

Privratsky JR,Zhang J,Lu X,Rudemiller N,Wei Q,Yu YR,Gunn MD,Crowley SD

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Targeting macrophage and microglia activation with colony stimulating factor 1 receptor inhibitor is an effective strategy to treat injury-triggered neuropathic pain.

Molecular pain

Lee S,Shi XQ,Fan A,West B,Zhang J

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon Oct 22 00:00:00 EDT 2018

Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.

Nature communications

Kim DH,Park HJ,Lim S,Koo JH,Lee HG,Choi JO,Oh JH,Ha SJ,Kang MJ,Lee CM,Lee CG,Elias JA,Choi JM

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Cell metabolism

Thwe PM,Pelgrom LR,Cooper R,Beauchamp S,Reisz JA,D'Alessandro A,Everts B,Amiel E

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Tue Sep 05 00:00:00 EDT 2017

A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.

The Journal of clinical investigation

Berlato C,Khan MN,Schioppa T,Thompson R,Maniati E,Montfort A,Jangani M,Canosa M,Kulbe H,Hagemann UB,Duncan AR,Fletcher L,Wilkinson RW,Powles T,Quezada SA,Balkwill FR

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Wed Nov 20 00:00:00 EST 2019

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

The TLR7 agonist imiquimod induces anti-cancer effects via autophagic cell death and enhances anti-tumoral and systemic immunity during radiotherapy for melanoma.

Oncotarget

Cho JH,Lee HJ,Ko HJ,Yoon BI,Choe J,Kim KC,Hahn TW,Han JA,Choi SS,Jung YM,Lee KH,Lee YS,Jung YJ

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Tue Apr 11 00:00:00 EDT 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Thu Aug 03 00:00:00 EDT 2017

Antibiotic-induced perturbations in microbial diversity during post-natal development alters amyloid pathology in an aged APP_SWE/PS1_ΔE9 murine model of Alzheimer's disease.

Scientific reports

Minter MR,Hinterleitner R,Meisel M,Zhang C,Leone V,Zhang X,Oyler-Castrillo P,Zhang X,Musch MW,Shen X,Jabri B,Chang EB,Tanzi RE,Sisodia SS

25-7321 was used in Flow cytometry/Cell sorting to investigate how ABX-induced microbial diversity perturbations during post-natal stages of development coincide with altered host immunity mechanisms and amyloidosis in a murine model of AD.

Tue Sep 05 00:00:00 EDT 2017

Scramblase TMEM16F terminates T cell receptor signaling to restrict T cell exhaustion.

The Journal of experimental medicine

Hu Y,Kim JH,He K,Wan Q,Kim J,Flach M,Kirchhausen T,Vortkamp A,Winau F

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon Nov 14 00:00:00 EST 2016

Reprogramming the immunological microenvironment through radiation and targeting Axl.

Nature communications

Aguilera TA,Rafat M,Castellini L,Shehade H,Kariolis MS,Hui AB,Stehr H,von Eyben R,Jiang D,Ellies LG,Koong AC,Diehn M,Rankin EB,Graves EE,Giaccia AJ

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2016

A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks.

Cell

Parnas O,Jovanovic M,Eisenhaure TM,Herbst RH,Dixit A,Ye CJ,Przybylski D,Platt RJ,Tirosh I,Sanjana NE,Shalem O,Satija R,Raychowdhury R,Mertins P,Carr SA,Zhang F,Hacohen N,Regev A

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

Enhancing the Quality of Antibodies to HIV-1 Envelope by GagPol-Specific Th Cells.

Journal of immunology (Baltimore, Md. : 1950)

Storcksdieck genannt Bonsmann M,Niezold T,Temchura V,Pissani F,Ehrhardt K,Brown EP,Osei-Owusu NY,Hannaman D,Hengel H,Ackerman ME,Streeck H,Nabi G,Tenbusch M,Überla K

25-7321 was used in Flow cytometry/Cell sorting to enhance the quality of antibodies to HIV-1 envelope by GagPol-Specific T helper Cells.

Sun Nov 15 00:00:00 EST 2015

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

Triclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses.

Toxicological sciences : an official journal of the Society of Toxicology

Marshall NB,Lukomska E,Long CM,Kashon ML,Sharpnack DD,Nayak AP,Anderson KL,Jean Meade B,Anderson SE

25-7321 was used in Flow cytometry/Cell sorting to examine the effects of dermal triclosan exposure on the skin and lymph nodes of mice and in a human skin model to identify mechanisms for augmenting allergic responses.

Tue Sep 01 00:00:00 EDT 2015

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

Helminth-induced interleukin-4 abrogates invariant natural killer T cell activation-associated clearance of bacterial infection.

Infection and immunity

Hsieh YJ,Fu CL,Hsieh MH

25-7321 was used in Flow cytometry/Cell sorting to reveal a previously unappreciated role for helminth-induced IL-4 in impairment of iNKT cell-mediated clearance of bacterial coexposure.

Thu May 01 00:00:00 EDT 2014

Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.

Vaccine

Goicochea MA,Zapata JC,Bryant J,Davis H,Salvato MS,Lukashevich IS

Published figure using TNF alpha monoclonal antibody (Product # 25-7321-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2012

Bioinformatics

Protein Aliases: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

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Gene Aliases: DIF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2

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UniProt ID: (Mouse) P06804

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Entrez Gene ID: (Mouse) 21926

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Function(s)

protease binding cytokine activity tumor necrosis factor receptor binding protein binding identical protein binding transcription regulatory region DNA binding

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TNF alpha Monoclonal Antibody (MP6-XT22), PE-Cyanine7, eBioscience™

TNF alpha Antibody (25-7321-82) in Flow

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25-7321-82

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