Hot-start PCR prevents the amplification of non-specific products, amplifies low abundance targets and offers convenient room temperature reaction setup. We offer different hot-start DNA polymerases to support your everyday research needs. This includes Thermo Scientific DreamTaq Hot Start DNA Polymerase, which is an enhanced hot start Taq DNA polymerase suitable for most PCR applications. DreamTaq Hot Start offers higher sensitivity, specificity, and yields compared to conventional hot start Taq DNA polymerase. 

Choose a hot-start DNA polymerase

 For everyday researchFor fast PCR
 DreamTaq Hot Start
DNA Polymerase		Phire Hot Start II
DNA Polymerase
Enhanced specificityYesYes
Sensitivity3 pg100 pg
Amplicon size–genomic/phage DNA≤6 kb/20 kb≤7.5 kb/20 kb
Activation timeImmediateImmediate
Cycling time60 s/kb10–15 s/kb
3′A overhang or blunt3′ABlunt
Stand-alone enzyme
Master mix

*Contains green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels.

Additional hot-start DNA polymerases

Resources

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