DNA Polymerases | Thermo Fisher Scientific

Mesophilic and thermophilic DNA polymerases for different polymerization reactions, DNA end-blunting and amplification, labeling and others

Properties of DNA polymerases

Polymerase	Applications	Template	Strand displace- ment
DNA Polymerase I	DNA labeling by nick-translation Second strand cDNA synthesis	DNA
Klenow Fragment	DNA blunting by fill-in 5'- overhangs Random-primed DNA labeling Labeling by fill-in 5'- overhangs of dsDNA DNA sequencing Site-specific mutagenesis Second strand cDNA synthesis	DNA
Klenow Fragment, exo-	Random-primed DNA labeling Labeling by fill-in 5'- overhangs of dsDNA Strand displacement amplification DNA sequencing	DNA
T4 DNA Polymerase	Blunting of DNA with 5'- or 3'- overhangs Blunting of PCR products with 3'-dA overhangs DNA labeling Site-directed mutagenesis Ligation-independent cloning	DNA
T7 DNA Polymerase	Purification of covalently closed circular DNA Primer extension reactions on long templates DNA 3'-end labeling Strand extensions in site directed mutagenesis Fill-in blunting of 5'-overhang DNA Second strand cDNA synthesis In situ detection of DNA fragmentation associated with apoptosis	DNA
Terminal deoxynucleotidyl transferase (TdT)	Production of synthetic homo-, heteropolymers Homo polymeric tailing of linear duplex DNA with 3'-OH terminus Oligodeoxyribonucleotide and DNA labeling 5'-RACE	ssDNA dsDNA ssRNA dsRNA	NA
Terminal deoxynucleotidyl transferase (TdT), Recombinant	Production of synthetic homo-, heteropolymers Homo polymeric tailing of linear duplex DNA with 3'-OH terminus Oligodeoxyribonucleotide and DNA labeling PCR, RNA analysis, genomic DNA cloning, 5' RACE, sequencing, and labeling of DNA probes	ssDNA dsDNA ssRNA dsRNA	NA
phi29 DNA Polymerase	Rolling circle amplification Multiple displacement amplification Whole genome amplification(WGA) DNA sequencing Protein-primed DNA amplification RNA-primed DNA amplification	DNA
Bsm DNA Polymerase	Loop-mediated isothermal amplification (LAMP) Whole genome amplification (WGA) Ramification amplification (RAM) Random-primed DNA labeling Labeling by fill-in 5'-overhangs of dsDNA	DNA