CTS Rotea Counterflow Centrifugation System Specifications

Instrument specifications

 

Easily fitting on a benchtop, the compact CTS Rotea system’s counterflow centrifugation technology was designed to gently and efficiently separate cells or aggregates based on size, and to concentrate particles with outer diameter (OD) >5 μm.

 

The proprietary Gibco DirectDraw extraction technology allows users to gently and precisely recover washed and concentrated cells from the processing chamber at the extremely low-volume output of 5 mL and is ideal when electroporation is the next step. In addition, the CTS Rotea system incorporates multiple sensors to monitor and protect the integrity of the closed system.
 

Attribute Description
Centrifugal force 3000 x g
Peristaltic pump flow rate 5–160 mL/min*
Centrifuge chamber volume 10 mL
Cell count per chamber load 5 x 10⁷–5 x 10⁹ cells**
Output cell concentration 5 x 10⁶–500 x 10⁶ cells/mL
Recommended input volume range 50 mL–20 L
Minimum output volume range 5 mL
Maximum fluid density 1.10 g/mL
Operating noise level <70 dBA
Dimensions (H x W x D)

16 x 11.4 x 20 in (body only), 30 x 11.4 x 20 in (with bag poles)

(40 x 29 x 50.8 cm (body only), 76.2 x 29 x 50.8 cm (with bag poles))
Weight 44 lb (20 kg)
Tablet accessory model Dell™ system, validated by Thermo Fisher Scientific for use with the CTS Rotea system

*Dependent on single-use kit type.

**Dependent on cell type. Centrifuge can be filled multiple times to process larger batches.

Note: Specifications and parameters above are cell type and protocol dependent. Instrument and Consumable Regulatory Support File available upon request at thermofisher.com/regulatory.

Single-use kit specifications

The CTS Rotea single-use kit provides flexibility for process development and seamless transfer to clinical-scale manufacturing.

Parameter Specification
Kit sterility Compatible with Luer connectors and sterile welding
Sterilization method Gamma sterilized
Tubing material DEHP-free PVC
Flow rates Standard kit: 5–110 mL/min
Hi-flow kit: 30–160 mL/min
Output cell concentration Up to 500 x 106 cells/mL

For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products.