The Impact of Vials on Biopharmaceutical Analysis

Vials have more of an impact on biomolecule analysis than you would think, and thus selecting the right vial for your application is important. Oligonucleotides, proteins, and peptides typically stick to the glass of regular vials, which can cause challenges with method robustness and recovery. Our HPLC Column Selection Guide allows you to easily identify application appropriate columns and compatible vials for your bioanalysis needs.

Protein and peptide samples

Hydrophobic proteins and peptides tend to create salt adducts with glass vials over time, reducing the recovery of certain proteins or peptides. Thermo Scientific SureSTART GOLD-Grade Vials have an ultra-low adsorption glass surface that enables trace-level analysis for strongly adsorbing analytes like proteins. These vials deliver the highest recovery rates with trisubstituted N-atoms and tertiary amines as well as the lowest levels of alkaline materials for less glass-wall interactions.

Bar graph plot of the peak area obtained after SEC- fluorescence analysis of AAV sample stored in plastic or glass vials at different points.

Nucleic acid samples

Nucleic acids, including oligonucleotides, typically form adducts with residual metals within the glass of a vial,  causing loss of recovery and robustness over time. To ensure the robustness of your method, Thermo Scientific SureSTART Polypropylene Vials should be used for oligonucleotide analysis.

Lipid nanoparticle applications

When working with lipid nanoparticle drug delivery systems, it is important to not only achieve the best sensitivity, but also assure robustness for small injection volumes. Thermo Scientific SureSTART High Recovery Glass Snap Top Microvials maximize injection volumes when analyzing <2 mL samples and enable a residual volume of 4 µL with their inner V-shaped bottom. These vials are designed to protect your most valuable samples and deliver consistent, reproducible results for your most demanding analyses when you have low sample volumes.

Select the right SureSTART vial and well plate for your application or instrument type

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