Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Hypersil GOLD HPLC and UHPLC Columns
Designed for optimum chromatography
Enjoy the range and capabilities of these state-of-the-art columns and gain confidence in the accuracy and quality of your analytical data. The Thermo Scientific Hypersil GOLD family of liquid chromatography (LC) columns is the culmination of 40 years of experience in the media and column product development and manufacturing.
Improved sensitivity
Outstanding peak shape results in greater sensitivity. When peaks exhibit tailing, peak height is reduced, therefore compromising the sensitivity of analysis. The highly symmetrical peaks provided by Hypersil GOLD columns enhance peak height and allow for optimized peak integration calculations. This can be particularly critical when low concentrations of an analyte are present, such as in an impurity assay.
Click to zoom
Conditions
- Column: Hypersil GOLD, 5μm, 150 × 4.6mm
- Mobile Phase: A: 0.1% ammonia pH 10.6
- B: Methanol + 0.1% ammonia
- Gradient: 5–100% B in 15 min
- Flow Rate: 1.0mL/min
- Injection Volume: 10μL
- Detection: UV @ 254nm
- Temperature: 30 °C
Hypersil GOLD column phase properties
| Phase | Phase Properties |
|---|---|
| Hypersil GOLD Peptide HPLC Columns | Consistent retention of all peptides, including hydrophilic ones, and resolve deamidated species |
| Hypersil GOLD C18 Selectivity LC Columns | Excellent peak shape for all analyte types. |
| Hypersil GOLD C8 LC Columns | Similar selectivity to C18 columns, but with reduced retention. |
| Hypersil GOLD C4 LC Columns | Similar selectivity to C18 and C8 columns, but with reduced retention. |
| Hypersil GOLD aQ LC Columns | Polar endcapped C18 columns, stable in 100% aqueous mobile phase, providing enhanced retention and resolution of polar analytes. |
| Hypersil GOLD PFP LC Columns | Perfluorinated phenyl columns offering alternative selectivity. The fluorine atoms around the phenyl ring enhance pi-pi interactions with aromatic molecules. |
| Hypersil GOLD Phenyl LC Columns | Excellent retention and unique selectivity for aromatic analytes. |
| Hypersil GOLD Cyano LC Columns | Alternative selectivity with lower hydrophobicity; can also be used for normal phase separations. |
| Hypersil GOLD Amino LC Columns | Can be used in reversed phase, normal phase, ion exchange, and HILIC modes; particularly useful for separating carbohydrates. |
| Hypersil GOLD AX LC Columns | Separation of smaller proteins, peptides, anionic species, and polar molecules. |
| Hypersil GOLD SAX LC Columns | A quaternary amino ion exchange ligand ideally suited to separating small polar organic analytes in aqueous mobile phases. |
| Hypersil GOLD Silica LC Columns | Separation of nonpolar and moderately polar organic compounds by normal phase chromatography. |
| Hypersil GOLD PEI HILIC LC Columns | Enhanced retention of polar and hydrophilic analytes that are problematic using reversed phase columns. |
Important product information
Hypersil GOLD HPLC columns technical guide
Hypersil GOLD columns are based on improved, highly pure silica and a novel proprietary derivatization and endcapping procedure using alkyl chain chemistry.
Hypersil GOLD HPLC columns phase overview
The capabilities of this state-of-the-art family of columns, with numerous chemistries and a range of particle sizes and hardware formats, meet the challenges of modern chromatography.
New changes to the US Pharmacopeia: Reduce time and solvent usage with newer technology columns
Learn how to modernize your methods to reduce time and solvent spend, while becoming more efficient and environmentally friendly.
2022/2023 HPLC/UHPLC Columns Catalog
Access the latest interactive version of our Chromatography Consumables & Columns Catalog. Our enhanced online version features easier access to product information, key application notes and brand-new selector tools.
Resources
Style Sheet for Global Design System
Style Sheet for Komodo Tabs
CMD SchemaApp code