HID NIMBUS Presto QNA System Reagents and Kits

Conquer your caseload   

 

The Applied Biosystems HID NIMBUS Presto QNA systems are specifically scripted, optimized, and validated for use with Applied Biosystems forensic extraction, quantification, and STR amplification kits (listed below). The rapid, hands-free processing and high-quality yields of the HID NIMBUS Presto QNA systems with even the most challenging samples—including bone, tooth, and touch DNA—and the automated quantitation, normalization, and amplification of the HID NIMBUS QNA systems help simplify the workflow, improve efficiency, and offer reliable results in forensic DNA analysis. With the HID NIMBUS Presto QNA System, three steps of the forensic casework workflow are carried out by one instrument. 

 

Extraction kits

  • PrepFiler & PrepFiler BTA kits

Quantification kit

  • Quantifiler Trio kit 

PCR kits 

  • GlobalFiler & GlobalFiler IQC kits
  • VeriFiler Plus kit
  • Yfiler Plus kit
  • NGM Detect kit 

PrepFiler and PrepFiler BTA Automated Forensic DNA Extraction Kits

PrepFiler and PrepFiler BTA automated forensic DNA extraction kits are specifically designed to improve the quantity and quality of DNA isolated from forensic samples, supporting the ability to obtain maximum information from downstream STR analysis.

Using a combination of uniquely structured magnetic particles and a multicomponent surface chemistry, these kits are optimized to provide extremely efficient DNA binding capacity and maximum DNA recovery from simple to challenging forensic samples, including bone, teeth, and touch/trace samples, as well as adhesive substrates.

Quantifiler Trio Automated DNA Quantification Kit

The HID NIMBUS Presto QNA systems are designed to prepare samples for quantification on either the QuantStudio 5 or 7500 real-time PCR systems using the Quantifiler Trio Automated DNA Quantification Kit.

This kit is specially designed to quantify the total amount of amplifiable human DNA in a sample and includes all the necessary reagents for detection, amplification, and quantification of three human-specific DNA targets, including improved male Y target to allow more sensitive male:female ratio detection and improved results in sexual assault casework.

The kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal or miniSTR) and helps streamline the workflow while increasing downstream analysis success rates.

GlobalFiler PCR Amplification Kits

The GlobalFiler PCR Amplification Kits combine reduced amplification time with exceptional discrimination power, even on the most challenging sample types. The kit, with 10 mini-STR markers, incorporates the most commonly used loci—all in a single multiplex kit. It contains all markers recommended for inclusion by the CODIS Core Loci Working Group and those markers commonly used in Europe, including SE33. The multiplex selection of markers reduces the risk of adventitious matches while enabling more effective cross-border data sharing.  The GlobalFiler kit is approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

VeriFiler Pus PCR Amplification Kit

The VeriFiler Pus PCR Amplification Kit offers excellent genotyping results from single-source and challenging casework samples using a 25-marker STR multiplex assay that includes two gender determination markers, as well as the highly discriminatory Penta D and Penta E markers. VeriFiler Plus kits are approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

With 11 mini-STRs, VeriFiler Plus kits are specifically designed for challenging sample types: touch, inhibited, or degraded samples, with improved sensitivity and robustness to inhibition. In addition, an Internal Quality Control (IQC) system assesses for the presence of inhibitors in the sample to enable distinction between degraded and inhibited samples which can help forensics analysts make better decisions on how to further process problematic samples. The IQC system also verifies PCR amplification.

YFiler Plus PCR Amplification Kit

The YFiler Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA. It offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples. The kit is a multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.

NGM Detect PCR Amplification Kit

The NGM Detect PCR Amplification kit facilitates exceptional data quality and robustness along with powerful discrimination to support European cross-border data sharing initiatives. The NGM kits amplify all SGM Plus kit loci together with additional loci in the expanded European Standard Set as recommended by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP).

The NGM Detect kit, with 7 mini-STR markers, offers excellent sensitivity and maximizes information recovery, even from degraded casework samples. It also contains an Internal Quality Control (IQC) system, which assesses for the presence of inhibitors in the sample to enable distinction between degraded and inhibited samples, which can help forensics analysts make better decisions on how to further process problematic samples.

Ordering information

For Research, Forensic, or Paternity Use Only. Not for use in diagnostic procedures.