Liver Microsomes, S9 Fractions and Cytosol

Subcellular fractions, derived from the endoplasmic reticulum of liver, contain a rich variety of metabolic enzymes for assessing the in vitro metabolism of drug candidates (Table 1) and are suitable for a variety of experiments:

• Cytochrome P450 inhibition studies
• Metabolic stability
• Cytochrome P450 phenotyping
• Metabolite characterization

50 donor human liver microsome pool
Designed to maximize functionality for all your study types- suitable for inhibition, clearance, or metabolic identification.

• Equal representation of all 50 donors
• Activities representing the typical patient population

11 specialty human liver microsome pools
Prepared with 5 to 20 donors each and pooled for your convenience according to age, gender, body mass index, high 3A activity, and high 2D6 activity.

Customized microsome pools
Our scientists will create a customized pool specifically for your unique research needs using our large, fully characterized single-donor database.

Life Technologies provides liver subcellular fractions from a variety of tox species, including human, nonhuman primates (Cynomolgus Monkey and Rhesus Monkey), dog (Beagle), rat (Sprague-Dawley), mouse (CD-1),  and trout (Oncorhynchus mykiss).  Custom species are available upon request. 

Each product contains an average representative pool of donors.  Human microsome pools are fully characterized (K_m and V_max) according to GLP standards for major cytochrome P450 activities and select Phase II enzymes using FDA recommended substrates (Tables 2-3).