ProQuantum High-Sensitivity Immunoassays 

ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification (Figure 2).

ProQuantum Software analyzes target-specific protein quantitation results for all ProQuantum high-sensitivity immunoassay kits. The software provides end-to-end support starting with step-by-step guidance to prepare an assay run including setting up the standard curve and plate layout for 96- or 384-well plates. The analysis features include standard curve qualification, with built-in outlier detection, interpolation of unknown samples for quantitation, and group-wise statistical analysis.

• Easily design your experimental 96 or 384-well plate run with automatic calculations for standard curve dilutions and suggested plate plans
• Get customized reaction volumes and lab instructions based on your plate plan (Cloud only)
• 4 or 5PL algorithm standard curve fit with built-in outlier detection
• Easily view unknown sample concentrations from standard curve graphs or in table format
• Statistical group-wise comparison
• Export data to .pdf, .xls or GraphPad Prism

Figure 3. Intuitive software for easy and comprehensive data analysis

To try the software with a ProQuantum data file, use this sample .eds data file to upload. Please click on create experiment (Figure 4), Type in Cat. No. A35601 for the autopopulated default settings, select duplicate and click next. Create a new plate plan according to this image (Figure 5) by typing in the number 30 in the Samples text box. Then, import the .eds file (Figure 6) and select your plate plan to apply for analysis.

Thermo Fisher Scientific is committed to designing our products with the environment in mind—it’s part of how we enable our customers to make the world healthier, cleaner, and safer. This fact sheet provides the rationale behind the environmental claim that Invitrogen ProQuantum Immunoassay Kits generate less waste and have more sustainable packaging than traditional ELISA kits. To learn more download the green features fact sheet