Gibco Viral Vector HEK Media and Feed Panel | Thermo Fisher Scientific

Identify an optimal formulation for your target cell line

Manufacturers of adeno-associated viral (AAV) vectors need to scale up quickly and cost-effectively to meet the increasing demand from the gene therapy market. But testing and optimizing multiple catalog media takes time and expertise. What if you had a panel of media and an expert in media optimization to help you?

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The Gibco Viral Vector HEK Media Panel provides a diverse offering of media to accelerate gene therapy development and production. The media panel can be used for a broad range of HEK293 cells. It is not dependent on specific transfection reagents or on specific techniques. As a result, the ready-to-use format can help identify a formulation for your target cell line that is optimized for higher titers, is scalable, and may be further customized to improve performance.

Throughout the evaluation process our scientists will provide technical support. Acting as an extension of your R&D team, our scientists will provide process consultation, data analysis, and a summary report to expedite media optimization and cGMP manufacturing.

What’s new: Gibco Viral Vector HEK Feed Panel

Gibco Viral Vector HEK Feed Panel bottles

Confidently match the right feed with your cell line

The HEK Feed Panel provides a diverse offering of formulations to help optimize your viral vector processes. This library of feeds is designed for HEK cells and has been tested in multiple media, including the Gibco Viral Vector HEK Media Panel. The feeds included in the panel can help meet your cells’ nutritional needs for you to scale-up to production.

Scaling up with the HEK Feed Panel can provide:

Diversity—distinct formulations for various cell lineages
Simplicity—three ready-to-use concentrated feed formulations
Productivity—Potential titer improvement over batch
Expertise—dedicated field application scientist support through evaluation for customization, experimental design, and troubleshooting

Click the links to request more information on the Panel or download the new Gibco Viral Vector HEK Feed Panel Flyer

Viral Vector HEK Media Panel screen of one HEK cell line

Responses to the media panel vary based on HEK cell line origin and adaptation strategy performed. A HEK293 cell line grown in a chemically defined medium was adapted to the five media formulations in the Viral Vector HEK Media Panel and FreeStyle F17 Expression Medium. AAV2 titers were generated using triple transfection with a polymer-based reagent (biological triplicate performed). No titer enhancer was used to assess the effect of base media compared to the control titer obtained in FreeStyle F17 Expression Medium as indicated by the blue line (x=1).