Affinity Columns | Thermo Fisher Scientific

Decorative image of POROS family of chromatography columns

POROS analytical chromatography columns are used for the quantification and assessment of purity of biomolecules throughout cell culture and purification processes. POROS A, G, AL, EP, OH, and POROS CaptureSelect columns are polymeric packings designed for high-resolution separation, specifically affinity chromatography of antibodies, proteins, polynucleotides, and other biomolecules with user-immobilized ligands. The resins consist of cross-linked poly[styrene-divinylbenzene] (DVB) flow-through particles for rapid mass transport. This base bead is coated with a cross-linked polyhydroxylated polymer (POROS OH), which is then activated with aldehyde (POROS AL) or epoxide (POROS EP) functional groups.

Illustration of binding sites for affinity ligands Protein A, Protein G, and Protein L

Figure 1. POROS A, G, and CaptureSelect ligands target multiple IgG epitopes. Figure 1 illustrates the binding sites for affinity ligands Protein A, Protein G, and Protein L. Novel POROS ligands provide alternative specificities that enable rapid binding for the quantitation or purification of engineered immunoglobulins.

Note: POROS affinity chromatography columns are shown in orange.

Ordering information

POROS A, G, and ligands are available in convenient prepacked HPLC columns. The column range addresses analytical and preparative requirements for the quantitation and purification of immunoglobulins or Fc-fusion or HSA-fusion proteins.

Affinity resin columns

POROS A 20 µm Columns

POROS A 50 micron Columns:

POROS G 20 µm Columns

POROS HE 50 – Columns

POROS MC µm Columns

Activated affinity

POROS AL 20 µm Columns

POROS EP 20 µm Columns

POROS OH 50 Micron columns

POROS AL 20 Activated Affinity Resins (Aldehyde)

POROS HP2