There are at least three distinct mitogen-activated protein kinase (MAPK) signaling modules which mediate extracellular signals into the nucleus to turn on the responsive genes in mammalian cells, including ERK, JNK, and p38 kinase. The MAPK signaling pathway is essential in regulating many cellular processes including inflammation, cell differentiation, cell proliferation and death. The role of MAPK pathway in cancer, immune disorders and neurodegenerative diseases has been well recognized.

Life Technologies offers antibodies, ELISAs, Luminex® multiplex assays and growth factors for key targets in the MAPK signaling cascade. 

Find MAPK signaling pathway primary antibodies, antibody pairs, ELISAs and Luminex® assays by protein target: 


Targets A–F

 

Targets G–L

 

Targets M–Z

    

Key MAPK Pathway Target

One key target, p38 MAPK, is activated in response to a variety of extracellular stimuli including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), anisomycin, UV light, and growth factors. The activation of p38 MAPK is mediated by several upstream kinases including MAP kinase kinase 3 (MKK3), MAP kinase kinase 6 (MKK6) and MAP kinase kinase 4 (MKK4, also known as SEK1 and JNKK1). These kinases phosphorylate p38 MAPK at threonine 180 and tyrosine 182, resulting in p38 activation.

Western Blot Analysis

Western Blot with with Anti-p38MAPK Antibody

Western blot analysis of p38 MAPK [pTpY180/182] antibody peptide competition.

Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% polyacrylamide gel and transferred to PVDF. Prior to incubation with p38 MAPK [pTpY180/182] antibody, the immobilized membranes were incubated with the (lane 1) peptide immunogen, (lane 2) a generic phosphothreonine-containing peptide, (lane 3) a generic phosphotyrosine-containing peptide, (lane 4) the non-phosphorylated peptide corresponding to the phosphopeptide, (lane 5) no peptide, (lane 6) the phosphopeptide derived from the corresponding region of JNK1/2, and (lane 7) the phosphopeptide derived from the corresponding region of ERK1/2. The data show that only the phosphopeptide corresponding to p38 MAPK [pTpY180/182] blocks the antibody signal, thereby demonstrating the specificity of the phosphospecific antibody.

Immunohistochemistry

IHC with Anti-p38MAPK Antibody

Immunohistochemistry of human lung carcinoma tissue labeled with rabbit anti-ERK1/2 [pT185/pY187].

FFPE human lung carcinoma tissue was labeled with rabbit anti-ERK1/2 [pT185/pY187] (5 µg/ml). Tissues were pretreated with EDTA, then detected with SuperPicTure™ Polymer DAB . Images were taken at 20x magnification. Note nuclear and cytoplasmic staining in tumor cells.

ELISA Quantification

ELISA Quantification of p38MAPK

ELISA quantification of total p38 MAPK and phosphorylated p38 MAPK [pTpY180/182] in vitro.

Recombinant p38 MAPK was phosphorylated in vitro using MKK6 enzyme. Non-phosphorylated p38 MAPK was used as control. The phosphorylated and non-phosphorylated p38 MAPK were analyzed with p38 MAPK [pTpY180/182] ELISA and p38 MAPK (Total) ELISA. Both forms of the p38 MAK protein were detected using the total ELISA whereas only the phosphorylated p38 MAPK protein was detected with the phosphospecific ELISA.

Find MAPK Protein Targets A–F

TargetABfinity™ Monoclonal AntibodiesRecombinant Oligoclonal AntibodiesOther Primary Antibodies**Antibody PairsELISAProteinsLuminex®
Assays
BDNF     PHC7074 LHC7071
BMP2710022      
c-Met700261  CHO0285KHO0251  
c-Met (Soluble)   CHO0315KHO2031  
c-Met [pYpYpY1230/34/35]700139   KHO0281  
c-Raf [pS289/296/301]  A14039
(Rab)		    

 

TargetABfinity™ Monoclonal AntibodiesRecombinant Oligoclonal AntibodiesOther Primary Antibodies**Antibody PairsELISAProteinsLuminex®
Assays
CREB 710149     
CREB [pS133]700129   KHO0241  
CREB Total    KHO0231  
Cytokine 30-plex (Human)      LHC6003
(Polystyrene)
LHC6003M
(Magnetic)
EGF   AHG0064
(Rab)		 

KHG0061
KHG0062

PHG0311
PHG0313
PHG0314
PHG0315

 LHG0061
EGFR   AHR5072
(Ms)		 KHR9061  
EGFR [pY1068]701053   KHR9081 LHR9081
EGFR [pY1173]    KHR9071  
ERK1  138600
(Ms)		    
ERK1/2  44654G
(Rab)
136200
(Ms)		 KHO0081 LHO0231
ERK1/2 [pTpY185/187]700012 44680G
(Rab)		 KHO0091 LHO0241
ERK1/2 [pTpY202/204]  368800
(Rab)		    
ERK5/BMK1  44688G
(Rab)		    
ERK5/BMK1 [pTpY218/220]  44612G
(Rab)		    
FAK701094710119     
FAK [pT925]  A14010
(Rab)		    
FGFbasic    KHG0021
KHG0022		PHG0021
PHG0023
PHG0024
PHG0026
PHG0264		 LHG0021
**Rab=rabbit polyclonal IgG antibody. Ms=mouse monoclonal IgG antibody

Find MAPK Protein Targets G–L

TargetABfinity™ Monoclonal AntibodiesRecombinant Oligoclonal AntibodiesOther Primary Antibodies**Antibody PairsELISAProteinsLuminex®
Assays
IGF1-R 710065    LHO0491
IGF-1R [pYpY1135/1136]      LHO0501
IL-1α  AHC0912
(Ms)		 KAC1191PHC0011
PHC0015
PHC0017		LHC0811
IL-1ß    CHC1213KHC0011
KHC0012
KHC0014		PHC0811
PHC0815
PHC0816		LHC0011

 

TargetABfinity™ Monoclonal AntibodiesRecombinant Oligoclonal AntibodiesOther Primary Antibodies**Antibody PairsELISAProteinsLuminex®
Assays
IL-2701080 RHCIL220
(Rt)
AHC0722
(Ms)		 CHC1243KHC0021
KAC1241		PHC0021
PHC0023
PHC0026
PHC0027		LHC0021
IL-6 710042AHC0562
(Ms)		CHC1263KAC1261
KHC0061
KHC0061C
KHC0062
KHC0063
KHC0064
KHC0064C		PHC0061
PHC0063
PHC0064
PHC0065
PHC0066		LHC0061
LHC0063
IR      LHR9111
IR (B-subunit)    KHR9111  
IR [pY1158]    KHR9121  
IR [pY1328]    KHR9151  
IR [pY1334]    KHR9161  
IR [pY972]    KHR9141  
IR [pYpY1162/1163]    KHR9131 LHR9131
IRAK1700025      
IRAK4700026      
IκBα [pS32]    KHO0221  
JIP1 (N-Term)  345200
(Rab)		    
JIP1/2 (SH3)  345300
(Rab)		    
JNK 710075     
JNK1701048 359800
(Ms)		    
JNK1/2  AHO1362
(Ms)		 KHO0121 LHO0071
JNK1/2 [pTpY183/185]  44682G
(Rab)		 KHO0131  
JNK2  AHO1362
(Ms)		    
**Ms=mouse monoclonal IgG antibody; Rab=rabbit polyclonal IgG antibody.  Rt=rat monoclonal IgG antibody

Find MAPK Protein Targets M–Z

TargetABfinity™ Monoclonal AntibodiesRecombinant Oligoclonal AntibodiesOther Primary AntibodiesAntibody PairsELISAProteinsLuminex®
Assays
MAPKAPK2  AHO1242
(Ms)		    
MAPKAPK2 [pT334]  44516G
(Rab)		    
MEK1 [pS298]  44460G
(Rab)		    
MEK1 [pT286]  A14036
(Rab)		    

 


TargetABfinity™ Monoclonal AntibodiesRecombinant Oligoclonal AntibodiesOther Primary AntibodiesAntibody PairsELISAProteinsLuminex®
Assays
MEK1 [pT292]  44458G
(Rab)		    
MEK1 [pT386]  44462G
(Rab)		    
MEK1/2 [pS222]  44452
(Rab)		    
MEK1/2 [pSpS218/222]/222/226  44454G
(Rab)		    
MEK2 [pT394]  44466G
(Rab)		    
MEK3  326900
(Ms)		    
MEK4 [pSpT257/261]  44474
(Rab)		    
MEK7 [pSpT271/275]  44478
(Rab)		    

  
    
MKK7  327000
(Ms)		    
NFκB    KHO0371  
p38 -Beta 2 MAPK  338700
(Ms)		    
p38 MAPK  331300
(Ms)		 KHO0061 LHO0031
p38 MAPK [pTpY180/182]701057 44684G
(Rab)		CHO0075KHO0071  
p53 [pT81]  A13988
(Rab)		    
Pyk2 [pY402]700632710087     
RSK1  AHO1472
(Ms)		    
RSK1 [pSpS221/227]  44924G
(Rab)		    
Shc [pY239/240]  A13980
(Rab)		    
STAT2701105710135     
STAT3 [pY705]    KHO0481  
STAT5a    KHO0751  
STAT5a [pY694]    KHO0761  
STAT5b [pY699]    KHO5721  
STAT6701110710140     
STAT6 [pY641]700247 44375M
(Ms)		    
TGFb1    CHC1683 PHG9202
PHG9203
PHG9204
PHG9211
PHG9214		 
TNFa 710162 CHC1753KHC3011PHC3011
PHC3013
PHC3015
PHC3016		LHC3011
**Rab=rabbit polyclonal IgG antibody. Ms=mouse monoclonal IgG antibody

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.