KingFisher Sample Purification System Sample Data

Nasopharyngeal swab specimens were spiked with 250 copies/mL (left) or 750 copies/mL (right) of SARS-CoV-2 viral RNA (1x or 3x the limit of detection, respectively), and RNA was extracted from 200 μL or 400 μL of the spiked sample in triplicate using the MagMAX Viral/Pathogen Kit (Cat. No. A42352) with (+) or without (–) Wash 3. After elution in 50 μL, the samples were assayed by qRT-PCR for four RNA targets (colored symbols) in triplicate. C_t values for each target were highly consistent (symbols cluster closely) and virtually identical between sample volumes and wash conditions, validating the streamlined lower-volume protocols. All negative samples had C_t values of “Undetected” (data not shown).

A KingFisher Duo Prime was used to isolate DNA from two donors’ whole blood using the MagMAX Multi-Sample Ultra 2.0 Kit (Cat. No. A36570). Samples >400 µL were processed using the large-volume protocol in a 24-well plate, while samples 50–400 µL were processed using the small volume protocol in a 96-well plate. (No sample volume normalization was needed, even when 50 µL and 400 µL samples were run at the same time on the same plate.) Run time was 45 minutes. (Top) Recovery volumes were linear across both small and large volume inputs. (Bottom) A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀ ratios demonstrated high purity across sample volumes.

Total nucleic acid from fecal, urine, and saliva samples from two donors was isolated using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit (Cat. Nos. A42357 and A42358) on the KingFisher Flex, submitted for shotgun metagenomic sequencing, and compared with known microbial profiles. The figure shows abundance heat maps for selected microbiota for all samples. Results confirmed that each body habitat harbored different dominant signature taxa, with only a few shared species, mostly between urine and saliva. In each community, there was substantial overlap between donors but also marked individual differences.