RNA cloning is used to study small RNAs, such as microRNAs and mRNA. Cloning of mRNA and microRNA requires that they be isolated from cellular samples or biofluids.

Obtaining high-quality, intact RNA is the first and often the most critical step in performing RNA cloning.

RNA cloning workflow for protein expression and functional analysis

Flow diagram for RNA cloning. First, RNA is isolated from tissues or cells, then reverse-transcription PCR is performed to generate a cDNA. The cDNA is amplified and placed into the desired plasmid/vector, followed by transformation, plasmid purification, and finally analysis by any number of methods.

mRNA capture for RNA cloning

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Messenger RNA (mRNA) molecules are important for gene expression. RNA cloning is one method of investigating functional differences in mRNA. Structurally, mRNA is distinct from other types of RNA (tRNA, rRNA, small RNAs, total RNA) due to their polyadenylated (poly(A)) tail, so selective targeting of mRNA by their poly(A) tails facilitates enrichment.

The Dynabeads mRNA DIRECT kit takes advantage of this unique characteristic of mRNA and combines it with Dynabeads magnetic bead technology.

Dynabeads mRNA DIRECT kit can help you:

  • Save money and lab space—kit can be used for a broad range of sample types including blood, serum, and plasma; mammalian, amphibian, fish, plant, and insect tissues; FFPE samples, and yeast
  • Save time—15-minute procedure yields pure, intact mRNA
  • Reduce extra steps for these downstream applications—recovered mRNA is suitable for RNA cloning, cDNA library construction, quantitative RT-PCR, RACE, SAGE, ribonuclease protection assays, subtractive hybridization, and primer extension

Learn more about mRNA extraction and enrichment methods

Ordering information for mRNA capture kits

MicroRNA isolation

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MicroRNAs (miRNAs) are a class of small RNAs that regulate protein translation. Much effort has gone into understanding how, when, and where miRNAs are produced and function in cells, tissues, and organisms. Isolating the mature miRNA fraction is the first critical step in cloning these small regulatory molecules.

Learn more about miRNA cloning
Compare microRNA and small RNA isolation kits

MicroRNA isolation kits offer efficient recovery of miRNA

Cellular RNA can greatly vary in size, but not all total RNA kits effectively recover all sizes. Traditional RNA isolation methods are not well suited for isolation of small RNAs, including microRNAs (or miRNAs). Variations of two methods, chemical (organic) extraction and solid-phase extraction, have been adapted for miRNA isolation. The mirVana miRNA Isolation Kit procedure combines the advantages of organic extraction and solid-phase extraction, while avoiding the disadvantages of both.

The mirVana miRNA isolation kit effectively recovers all RNA, allowing you to enrich for microRNA. High yields of ultra-pure, high quality, miRNA molecules can be prepared in about 30 minutes.

Benefits of mirVana miRNA Isolation Kit for miRNA isolation

  • Efficient isolation of small RNA-containing total RNA
  • Enrich for small RNA <200 nt to increase sensitivity in downstream analyses
  • Ideal for miRNA, siRNA, shRNA, and snRNA analysis

Ordering information for mirVana miRNA isolation kits

Resources
Technical notes
Stylesheet for Classic Wide Template adjustments

For Research Use Only. Not for use in diagnostic procedures.

Stylesheet for Classic Wide Template adjustments