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Technical notes

How-To Videos

How to setup for Invitrogen™ QuantiGene™ Assays: Equipment, assay components, and storage conditions

How to calibrate incubators for the Invitrogen™ QuantiGene™ Singleplex and Plex Assays

How to prepare cell lysate and determine optimal sample input for Invitrogen™ QuantiGene™ Assays

How to set up and run an Invitrogen™ QuantiGene™ Singleplex Assay

Recent publications

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ApplicationTitleYear
Virus hemocytesAntisense oligonucleotide targeting CD39 improves anti-tumor T cell immunity. 2019
Mouse striatumCoxiella burnetiiRpoS Regulates Genes Involved in Morphological Differentiation and Intracellular Growth. 2019
Human A498Expression Levels of microRNAs miR-93 and miR-200a in Pancreatic Adenocarcinoma with Special Reference to Differentiation and Relapse-Free Survival 2019
Mouse cortexFolate receptor-beta expression as a diagnostic target in human & rodent nonalcoholic steatohepatitis 2019
Human MV-4-11MicroRNA-92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells 2019
Bacteria pond sedimentPreclinical Characterization of NVR 3-778, a First-in-Class Capsid Assembly Modulator against Hepatitis B Virus 2019
Human HCC1954, SKBR3, temporal-lobe tissueRegulation of ovarian cancer G protein-coupled receptor-1 expression and signaling 2019
Human BT474Structure-guided discovery of a novel, potent, and orally bioavailable 3,5-dimethylisoxazole aryl-benzimidazole BET bromodomain inhibitor 2019
Human hepatocytesArtificial miRNAs Reduce Human Mutant Huntingtin Throughout the Striatum in a Transgenic Sheep Model of Huntington's Disease 2018
Human, Mouse, Ovine cytotrophoblast cells, liver, kidney, striatum, plasmaComparison of partially and fully chemically-modified siRNA in conjugate-mediated delivery in vivo 2018
Human breast cancerDiscovery of TRPM8 Antagonist ( S)-6-(((3-Fluoro-4-(trifluoromethoxy)phenyl)(3-fluoropyridin-2-yl)methyl)carbamoyl)nicotinic Acid (AMG 333), a Clinical Candidate for the Treatment of Migraine 2018
Human, Mouse, NHP multiple primary tissuesDiverse lipid conjugates for functional extra-hepatic siRNA delivery in vivo 2018
Non-human primate brainEfficacy of NVR 3-778, Alone and In Combination With Pegylated Interferon, vs Entecavir In uPA/SCID Mice With Humanized Livers and HBV Infection 2018
Rat plasma, serummRNA treatment produces sustained expression of enzymatically active human ADAMTS13 in mice 2018
Human metastatic clear cell renal cell carcinomaNuclear Localization of Huntingtin mRNA Is Specific to Cells of Neuronal Origin 2018
Mouse, Non-human primate liver, plasmaQuantitative Systems Pharmacology Model of hUGT1A1-modRNA Encoding for the UGT1A1 Enzyme to Treat Crigler-Najjar Syndrome Type 1 2018
Bacteria water samplesRNAi modulation of placental sFLT1 for the treatment of preeclampsia 2018
Rat plasma, liverSafety Evaluation of Lipid Nanoparticle-Formulated Modified mRNA in the Sprague-Dawley Rat and Cynomolgus Monkey 2018
Human HeLaThe immune response of Mytilus edulis hemocytes exposed to Vibrio splendidus LGP32 strain: A transcriptomic attempt at identifying molecular actors 2018
Human liverTransvascular Delivery of Hydrophobically Modified siRNAs: Gene Silencing in the Rat Brain upon Disruption of the Blood-Brain Barrier 2018
Non-human primate liverA Unique Approach to Design Potent and Selective Cyclic Adenosine Monophosphate Response Element Binding Protein, Binding Protein (CBP) Inhibitors 2017
Non-human primate boneBDNF val66met Polymorphism Impairs Hippocampal Long-Term Depression by Down-Regulation of 5-HT3 Receptors 2017
Non-human primate spleenGNE-781, A Highly Advanced Potent and Selective Bromodomain Inhibitor of Cyclic Adenosine Monophosphate Response Element Binding Protein, Binding Protein (CBP) 2017
Non-human primate aortic branchHDX reveals the conformational dynamics of DNA sequence specific VDR co-activator interactions 2017
Non-human primate skinLoading of Extracellular Vesicles with Chemically Stabilized Hydrophobic siRNAs for the Treatment of Disease in the Central Nervous System 2017
Mouse lungLong noncoding RNA HULC modulates the phosphorylation of YB-1 through serving as a scaffold of extracellular signal-regulated kinase and YB-1 to enhance hepatocarcinogenesis 2017
Non-human primate ovary, pancreasSynthesis and Evaluation of Parenchymal Retention and Efficacy of a Metabolically Stable O-Phosphocholine-N-docosahexaenoyl-l-serine siRNA Conjugate in Mouse Brain 2017
Human hepatocytesThe Smc5/6 Complex Restricts HBV when Localized to ND10 without Inducing an Innate Immune Response and Is Counteracted by the HBV X Protein Shortly after Infection 2017
Human A549, liverA High-Throughput Method for Direct Detection of Therapeutic Oligonucleotide-Induced Gene Silencing In Vivo 2016
Human pancreatic tissueBiliary Elimination of Pemetrexed Is Dependent on Mrp2 in Rats: Potential Mechanism of Variable Response in Nonalcoholic Steatohepatitis 2016
Mouse fallopian tube, fat, heart, intestineBioprinting of 3D Convoluted Renal Proximal Tubules on Perfusable Chips 2016
Mouse liverComparison of the QuantiGene 2.0 Assay and Real-Time RT-PCR in the Detection of p53 Isoform mRNA Expression in Formalin-Fixed Paraffin-Embedded Tissues- A Preliminary Study 2016
Mouse neurons, muscle, pancreas, skin, spleenDendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose 2016
Mouse bladderDiscovery of a Potent and Selective in Vivo Probe (GNE-272) for the Bromodomains of CBP/EP300 2016
Mouse thymusDocosahexaenoic Acid Conjugation Enhances Distribution and Safety of siRNA upon Local Administration in Mouse Brain 2016
Virus HepG2Effect of nonalcoholic steatohepatitis on renal filtration and secretion of adefovir 2016
Mouse adrenal glandExosome-mediated Delivery of Hydrophobically Modified siRNA for Huntingtin mRNA Silencing 2016
Mouse kidneyMiR-99b-5p expression and response to tyrosine kinase inhibitor treatment in clear cell renal cell carcinoma patients 2016
Human WM-115The paraffin-embedded RNA metric (PERM) for RNA isolated from formalin-fixed, paraffin-embedded tissue 2016
Mouse heart, brainTherapeutic siRNA for drug-resistant HER2-positive breast cancer 2016
Mouse kidneyTranscriptional Profiling of Coxiella burnetii Reveals Extensive Cell Wall Remodeling in the Small Cell Variant Developmental Form 2016
Human MM.1STrimeprazine increases IRS2 in human islets and promotes pancreatic β cell growth and function in mice 2016
Human HDLM-2VEGF-A mRNA measurement in meningiomas using a new simplified approach: branched DNA and chemiluminescence 2016

For Research Use Only. Not for use in diagnostic procedures.