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Test to enable research for zika, dengue, and chikungunya viruses from one sample using a single-tube qPCR solution

Zika, dengue, and chikungunya viruses are single-stranded RNA arboviruses that spread to humans primarily through the bite of an infected mosquito of the Aedes genus. Infected individuals may show non-specific symptoms of fever, rash, joint pain, and/or conjunctivitis. Concerns about in utero transmission have been raised from studies that have shown that Zika virus may be linked to congenital disorders. Differentiation between these three arboviruses is imperative for epidemiologic and genetic surveillance of these vector-borne diseases.

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In May 2015, the Pan American Health Organization (PAHO) issued an alert regarding the first confirmed Zika virus infection in Brazil, and on February 1, 2016, the World Health Organization declared Zika virus a public health emergency of international concern. Local transmission has been reported in many other countries and territories globally. Zika virus will likely continue to spread (Centers for Disease Control and Prevention, 2016).

For more up-to-date information on Zika virus and the current outbreak, visit the CDC website or The Department of Health website.

Although chikungunya, dengue, and Zika viruses can be detected by testing serum for the presence of viral nucleic acid or virus-specific immunoglobulin M (IgM) and neutralizing antibodies, serological cross-reactivity is strong between Zika and dengue viruses. Pathogen detection using real-time PCR is strongly recommended.

Thermo Fisher Scientific offers three real-time PCR test kit options for these three arboviruses. Each of these kits offers the following:

  • Less than five minutes hands-on time
  • No cold-chain management required; ship and store at room temperature
  • Easy-to-use one-step operation. Simply add extracted RNA and qRT-PCR master mix to lyophilized, pre-filled tubes
  • Compatible with the Applied Biosystems QuantStudio 5, 6, and 7 Real -Time PCR Systems, and the Applied Biosystems 7500, 7500 Fast systems

Applied Biosystems real-time PCR solutions are part of a sample-to-results workflow. Benefits include the ability to:

  • Detect and differentiate multiple pathogens
  • Obtain reliable real-time PCR results—Accurate, sensitive, and specific
  • Reduce time-to-implementation—Accelerate and streamline your validation process with Analytical Validation Consulting Services
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Workflow
 

ArborovirusWorkflow

The Zika virus sequence used to develop these primers and probes was identified in a 2014 research paper by Pyke et al., titled “Imported Zika Virus Infection from the Cook Islands into Australia, 2014”.

 

For viral RNA purification, we recommend use of the Applied Biosystems MagMAX Pathogen RNA/DNA Kit.

Mosquito on skin

Battling Zika—Do we have your attention now?

Although it may feel like Zika is a new anomaly within the infectious disease community, its history is long, and the warning signs may have been appearing long before the virus made recent headlines.

Read about battling Zika ›

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Fortune Favors the Prepared Mind—Developing the Zika Triplex Assay

For decades, quantitative PCR (qPCR) assays have helped researchers run various research-specific experiments. As with all technology, the goal is to make technology as simple as possible and accessible.

About the assay ›

Video: The State of Zika

Learn more about how scientists in Brazil work to find answers around the Zika virus, and how Thermo Fisher Scientific worked diligently to develop the Zika real-time PCR research assay.

Watch video ›


Related products for arbovirus detection by real-time PCR
 

Perform real-time PCR (Research Use Only, RUO)

QuantStudio 5 Real-Time PCR System (96-well, 0.2 mL or 0.1 mL block)
QuantStudio 5, 6, and 7 Real-Time PCR Systems

The QuantStudio 5, 6, and 7 Real-Time PCR Systems are ideal for all levels of users who need a high-performance instrument with features for maximum control of their experiments. Includes built-in security features, locked workflows, user account management, and options for web browser–based or desktop analysis.

Visit the QuantStudio Real-Time PCR System Models for more information. Learn more ›

Isolate viral RNA

MagMAX Pathogen RNA/DNA Kit

The MagMAX Pathogen RNA/DNA Kit enables purification of RNA and DNA from viruses and easy-to-lyse bacteria and parasites using magnetic particle technology in a 96-well format. PCR inhibitors are effectively removed in the process, making the resulting nucleic acid ideal for real-time PCR and RT-PCR applications.


Ordering information
 

TaqMan Arbovirus Triplex Kit

TaqMan Arbovirus Triplex Kit, 0.2 mL

The kit contains lyophilized reagents to which RT-PCR master mix is added to provide a one-step workflow for detection of Zika, Dengue, and Chikungunya virus RNA from urine or serum.  

Contents include twelve 8-well strips tubes for 96 total reactions per kit.

Order now ›

 

TaqMan Arbovirus Triplex Control Kit

TaqMan Arbovirus Triplex Control Kit

The kit contains control material for detection of Zika, Dengue, and Chikungunya virus RNA from urine or serum.  

25 reactions per kit.

Order now ›

TaqMan Arbovirus Triplex Kit

TaqMan Arbovirus Triplex Kit, 0.1 mL

The kit contains lyophilized reagents to which RT-PCR master mix is added to provide a one-step workflow for detection of Zika, Dengue, and Chikungunya virus RNA from urine or serum.  

Contents include twelve 8-well strips tubes for 96 total reactions per kit.

Order now ›

For more up-to-date information, visit these WHO websites: Zika, dengue, and chikungunya

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For Research Use Only. Not for use in diagnostic procedures.

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