Antibody-Binding Plates

Protein A, G and L coated plates

Protein A, G and L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein A, G or L and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Protein A coated microplates:

• Binds strongly to IgG from human, rabbit, guinea pig, pig, dog, and rhesus monkey
• Binds strongly to mouse IgG2a, IgG2b, and IgG3
• Binds to Fc region of antibodies for optimal orientation

Protein G coated microplates:

• Binds strongly to IgG from many species including human, mouse, rabbit, sheep, and goat
• Binds only to IgG—no cross-reactivity with other antibody classes
• Binds to Fc region of antibodies for optimal orientation

Protein A/G coated microplates:

• Fusion protein containing four antibody-binding sites from Protein A and two from Protein G
• Binds to most IgG species because it combines the specificities of Protein A and Protein G
• Binds to Fc region of antibodies, ensuring optimal orientation
• Binds well using a wide range of pH

Protein L coated microplates:

• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE, and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV, and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat, or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding

Secondary antibody–coated plates

Antibody-coated plates are ideal for binding assays when available antibodies are in low quantities or denature upon direct adsorption to polystyrene plates. Because these plates are IgG-specific, purified antibodies are not required. Pierce Antibody Coated Plates may be used for direct, indirect, competitive, or sandwich assays and are available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays.