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Western blotting is a multi-step process that can take hours or one to two days to complete, depending on the methods used. Below are videos that detail the common steps used to perform a western blot, from separating proteins in protein gel electrophoresis to developing the blot.
Separate proteins based on molecular weight.
How to separate proteins using an Invitrogen precast SDS-PAGE gel
Transfer the protein from gel to a solid-support membrane.
How to perform a western wet tank transfer using the Invitrogen Mini Blot Module
How to perform a western semi-dry transfer using the Invitrogen Power Blotter and the Select Transfer Stacks
How to perform a western blot semi-dry transfer using the Invitrogen Power Blotter with precut membranes and filter papers
How to perform a western blot dry transfer using the Invitrogen iBlot 3 Western Blot Transfer Device
Block unreacted sites on the membrane to reduce the amount of nonspecific binding. Probe blot with primary and secondary antibodies.
How to probe a western blot
How to automate your western blot processing using the Invitrogen iBind Western systems
How to process western blots with the Invitrogen Bandmate Automated Western Blot Processor
Detect proteins based on the detection method that you choose.
How to detect your target proteins using chemiluminescent detection reagents
How to detect your target proteins using fluorescence-based reagents
Capture and analyze your image.
How to capture images with Invitrogen iBright Imaging Systems
How to analyze images with Invitrogen iBright Imaging Systems
Customize exposure routines with Invitrogen iBright Imaging Systems
Learn about the signal accumulation, multi-exposure, and region-of-interest Smart Exposure features of the Invitrogen iBright Imaging System.
How to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System for quantitative western blotting
This video demonstrates how to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System to capture and quantitate western blotting data using total protein normalization.
Stripping and reprobe the blot for another protein of interest.
How to strip and reprobe your western blot
Separate proteins based on molecular weight.
How to separate proteins using an Invitrogen precast SDS-PAGE gel
Transfer the protein from gel to a solid-support membrane.
How to perform a western wet tank transfer using the Invitrogen Mini Blot Module
How to perform a western semi-dry transfer using the Invitrogen Power Blotter and the Select Transfer Stacks
How to perform a western blot semi-dry transfer using the Invitrogen Power Blotter with precut membranes and filter papers
How to perform a western blot dry transfer using the Invitrogen iBlot 3 Western Blot Transfer Device
Block unreacted sites on the membrane to reduce the amount of nonspecific binding. Probe blot with primary and secondary antibodies.
How to probe a western blot
How to automate your western blot processing using the Invitrogen iBind Western systems
How to process western blots with the Invitrogen Bandmate Automated Western Blot Processor
Detect proteins based on the detection method that you choose.
How to detect your target proteins using chemiluminescent detection reagents
How to detect your target proteins using fluorescence-based reagents
Capture and analyze your image.
How to capture images with Invitrogen iBright Imaging Systems
How to analyze images with Invitrogen iBright Imaging Systems
Customize exposure routines with Invitrogen iBright Imaging Systems
Learn about the signal accumulation, multi-exposure, and region-of-interest Smart Exposure features of the Invitrogen iBright Imaging System.
How to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System for quantitative western blotting
This video demonstrates how to use Invitrogen No-Stain Protein Labeling Reagent and the iBright Imaging System to capture and quantitate western blotting data using total protein normalization.
Stripping and reprobe the blot for another protein of interest.
How to strip and reprobe your western blot
For Research Use Only. Not for use in diagnostic procedures.