Protein Expression Optimization

Nearly all molecular biology experiments start with cloned DNA. The gene can be a starting point for protein expression, structural analysis, or a functional experiment.

Researchers may derive their DNA from PCR using natural sources or purchase it from a premade clone collection. Buying the gene from an ORF collection is the fastest way if the gene of interest is available. However, you have to rely on preexisting material. Modifying these constructs can be time consuming and costly. Gene synthesis offers the opportunity to design every construct from scratch—tailored for the experiment. Your starting point can be any sequence information. This offers maximum flexibility to get the gene of choice in about 8–10 business days, 100% sequence verified, cloned into a vector of choice.

Synthesis of an optimized gene further enhances protein expression levels. In addition, optimized genes are resistant to RNA knock down.