The Thermo Scientific Pierce Standards are designed for a variety of applications, including sensitivity assessment, determination of digestion efficiency, chromatography assessment and/or as controls for sample analysis. Standards range from single component formulations to complex human-based digests. Each standard is provided with a Certificate of Analysis.

  • More choices—different standards to meet a variety of applications/needs
  • Stable—store for more than one year at the recommended temperature
  • Validated—all standards manufactured in an ISO 9001 facility measured and fully tested using Thermo Scientific Mass Spectrometers

Choose the right standard for your application

 Pierce BSA Digest Standard<Pierce 6-Protein Mixture StandardPierce Yeast Digest StandardPierce HeLa Protein Digest StandardPierce TMT11plex Yeast Digest Standard
 Pierce BSA Digest StandardPierce 6-Protein Mixture StandardPierce Yeast Digest StandardPierce HeLa Protein Digest StandardPierce TMT11plex Yeast Digest Standard
Components
(abbreviated)		BSA tryptic peptidesLysozyme, BSA, Cytochrome C, Alcohol dehydrogenase, B-galactosidase, Apotransferrin (multi-species)Yeast lysate peptide digestHeLa lysate peptidesYeast lysate peptide digest
FormatLyophilizedLyophilizedLyophilizedLyophilizedLyophilized
Primary applicationProtein sample controlChromatography assessmentMonitoring LC/MS system performanceComplex sample controlComplex sample for TMT-labeling
Complexity of standard+++++++++++++++
Recommended storage-20°C-20°C-20°C-20°C-20°C
 OrderOrderOrderOrderOrder
 
 Pierce Reserpine Standard for LC-MS
Components
(abbreviated)		Reserpine molecule
FormatLiquid
Primary applicationSensitivity assessment
Complexity of standard+
Recommended storage4°C
 Order
 Pierce Peptide Retention Time Calibration MixturePierce LC-MS/MS System Suitability StandardPierce Yeast Digest Standard
 Pierce Peptide Retention Time Calibration MixturePierce LC-MS/MS System Suitability Standard (7 x 5 Mix)Pierce HeLa Digest/PRTC Standard
Components
(abbreviated)		15 heavy tryptic peptides (yeast)7 yeast tryptic peptides (5 variations of light or heavy amino acids)Mixture of 10 µg of HeLa protein digest with 5 pmol PRTC peptides
FormatFrozen liquidFrozen liquidLyophilized
Primary applicationChromatography assessmentTargeted MS Assay controlMonitoring LC/MS system performance
Complexity of standard++++++++
Recommended storage-80°C-80°C-20°C
 OrderOrderOrder
 
 Pierce Intact Protein Standard Mix
Components
(abbreviated)		IGF-I LR3, Thioredoxin, Protein G, Carbonic Anhydrase II, Protein AG (chimeric), Exo Klenow
FormatLyophilized
Primary applicationTop down method development
Complexity of standard+++
Recommended storage-20°C
 Order
Protein digests (peptides)
 Pierce BSA Digest Standard<Pierce 6-Protein Mixture StandardPierce Yeast Digest StandardPierce HeLa Protein Digest StandardPierce TMT11plex Yeast Digest Standard
 Pierce BSA Digest StandardPierce 6-Protein Mixture StandardPierce Yeast Digest StandardPierce HeLa Protein Digest StandardPierce TMT11plex Yeast Digest Standard
Components
(abbreviated)		BSA tryptic peptidesLysozyme, BSA, Cytochrome C, Alcohol dehydrogenase, B-galactosidase, Apotransferrin (multi-species)Yeast lysate peptide digestHeLa lysate peptidesYeast lysate peptide digest
FormatLyophilizedLyophilizedLyophilizedLyophilizedLyophilized
Primary applicationProtein sample controlChromatography assessmentMonitoring LC/MS system performanceComplex sample controlComplex sample for TMT-labeling
Complexity of standard+++++++++++++++
Recommended storage-20°C-20°C-20°C-20°C-20°C
 OrderOrderOrderOrderOrder
Small molecule
 
 Pierce Reserpine Standard for LC-MS
Components
(abbreviated)		Reserpine molecule
FormatLiquid
Primary applicationSensitivity assessment
Complexity of standard+
Recommended storage4°C
 Order
Heavy peptide standards
 Pierce Peptide Retention Time Calibration MixturePierce LC-MS/MS System Suitability StandardPierce Yeast Digest Standard
 Pierce Peptide Retention Time Calibration MixturePierce LC-MS/MS System Suitability Standard (7 x 5 Mix)Pierce HeLa Digest/PRTC Standard
Components
(abbreviated)		15 heavy tryptic peptides (yeast)7 yeast tryptic peptides (5 variations of light or heavy amino acids)Mixture of 10 µg of HeLa protein digest with 5 pmol PRTC peptides
FormatFrozen liquidFrozen liquidLyophilized
Primary applicationChromatography assessmentTargeted MS Assay controlMonitoring LC/MS system performance
Complexity of standard++++++++
Recommended storage-80°C-80°C-20°C
 OrderOrderOrder
Intact protein
 
 Pierce Intact Protein Standard Mix
Components
(abbreviated)		IGF-I LR3, Thioredoxin, Protein G, Carbonic Anhydrase II, Protein AG (chimeric), Exo Klenow
FormatLyophilized
Primary applicationTop down method development
Complexity of standard+++
Recommended storage-20°C
 Order

Looking for a standard to measure digestion efficiency?

Thermo Scientific Pierce Digestion Indicator is a single, non-mammalian recombinant protein (26kDa) with five signature peptides for use in determining the digestion efficiency and reproducibility across multiple samples. The protein sequence and recommended peptides to monitor across samples are supplied with the product instructions.

Featured data

TMT11plex Yeast Digest Standard

The TMT11plex Yeast Digest Standard can be used to assess instrument readiness and protein quantification accuracy. The TMT11plex Yeast Digest Standard was used to assess MS instrument readiness on the precision of quantification of a housekeeping protein, ENO2. Protein abundance was scaled to the parental lines in Proteome Discover 2.2 software, with expected ratios of 1:1. A total of 500 ng of the Pierce TMT11plex Yeast Digest Standard was measured using a 50 min gradient on an UltiMate 3000 RSLCnano system with a synchronous precursor selection (SPS)-MS3 (SPS-MS3) method of the Orbitrap Fusion Lumos Tribrid Mass Spectrometer.

Thermo Scientific Pierce HeLa Digest Standard base peak chromatogram.
Thermo Scientific Pierce HeLa Digest Standard base peak chromatogram. Chromatogram of 200 ng Pierce HeLa Protein Digest Standard separated using a Thermo Scientific Acclaim PepMap 100 3 μm x 75 μm x 15 cm column (Product # 160321) with a 2-35% gradient (A: 0.1% FA in water, B: 0.1% FA in 100% acetonitrile) at 300 nL/min for 120 minutes and detected on a Thermo Scientific LTQ Orbitrap XL Mass Spectrometer.
Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific™ Vantage™ triple quadrupole mass spectrometer.
Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific Vantage triple quadrupole mass spectrometer. Chromatogram of three 5 μL loop injections of 100 fg/μL Pierce Reserpine on a Hypersil GOLD aQ 2.1x20 mm Javelin column with isocratic 300 μL/min flow of 70% methanol: 30% water: 0.05% formic acid (upper panel).The SRM transitions monitored were 609.3-195.1 and 609.3-448.2 m/z with a 0.2 FWHM Q1 peak width (lower panel).
TMT11plex Yeast Digest Standard
TMT11plex Yeast Digest Standard

The TMT11plex Yeast Digest Standard can be used to assess instrument readiness and protein quantification accuracy. The TMT11plex Yeast Digest Standard was used to assess MS instrument readiness on the precision of quantification of a housekeeping protein, ENO2. Protein abundance was scaled to the parental lines in Proteome Discover 2.2 software, with expected ratios of 1:1. A total of 500 ng of the Pierce TMT11plex Yeast Digest Standard was measured using a 50 min gradient on an UltiMate 3000 RSLCnano system with a synchronous precursor selection (SPS)-MS3 (SPS-MS3) method of the Orbitrap Fusion Lumos Tribrid Mass Spectrometer.

HeLa digest standard base peak
Thermo Scientific Pierce HeLa Digest Standard base peak chromatogram.
Thermo Scientific Pierce HeLa Digest Standard base peak chromatogram. Chromatogram of 200 ng Pierce HeLa Protein Digest Standard separated using a Thermo Scientific Acclaim PepMap 100 3 μm x 75 μm x 15 cm column (Product # 160321) with a 2-35% gradient (A: 0.1% FA in water, B: 0.1% FA in 100% acetonitrile) at 300 nL/min for 120 minutes and detected on a Thermo Scientific LTQ Orbitrap XL Mass Spectrometer.
Example SRM of reserpine
Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific™ Vantage™ triple quadrupole mass spectrometer.
Example selected reaction monitoring (SRM) of 500fg reserpine on a Thermo Scientific Vantage triple quadrupole mass spectrometer. Chromatogram of three 5 μL loop injections of 100 fg/μL Pierce Reserpine on a Hypersil GOLD aQ 2.1x20 mm Javelin column with isocratic 300 μL/min flow of 70% methanol: 30% water: 0.05% formic acid (upper panel).The SRM transitions monitored were 609.3-195.1 and 609.3-448.2 m/z with a 0.2 FWHM Q1 peak width (lower panel).

Resources

For Research Use Only. Not for use in diagnostic procedures.