Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Search Thermo Fisher Scientific
Find the best tools for your workflow from our broad selection of resins and formats for the purification of proteins and antibodies, or create a customized purification solution by covalent attachment of a ligand to one of our activated supports.
Our prepared and activated magnetic beads, magnetic agarose beads, standard and Superflow agarose resins, Applied Biosystem POROS resins, and other chromatography media enable protein purification at nearly any scale: assay, screening, batch, pilot, or process. In addition, we’ve developed a range of accessory products for all aspects of purification, including disposable columns and binding and elution buffers including disposable columns, binding and elution buffers, and endotoxin quantitation, detection, and removal products.
Ion Exchange Purification | Antibody Purification | Fusion Protein Purification | Biotin Affinity Purification | Protein Immobilization | |
Ligand or chemistry |
|
|
|
|
|
Base support |
|
|
|
|
|
Formats |
|
|
|
|
|
Endotoxin contamination is a common problem during protein purification. Learn more about our endotoxin quantitation, detection, and removal products.
Our magnetic agarose resins offer the high binding capacity of agarose combined with the automation capabilities of magnetic beads. We offer a variety of ligands, including Protein A/G, Ni-NTA, glutathione, and anti-FLAG, that can help enable the high-throughput purification of recombinants protein and antibodies.
Detect bacterial endotoxin with certainty using our Pierce Rapid Gel Clot Endotoxin Assays. Measure endotoxin levels as low as 0.1 EU/mL in just 20 minutes with our Chromogenic Endotoxin Quantitation Kit. Reduce endotoxin levels up to 99% in less than one hour using our Pierce High Capacity Endotoxin Removal Spin Columns, with protein recovery ≥85%.
We offer strong cation exchange (SCX) and strong anion exchange (SAX) resins and membranes. Our ion exchange (IEX) resins are rigid polymeric beads with covalent surface chemistries, which enable easier handling and packing while offering superior physical and chemical stability.
We offer multiple formats and pack sizes of Protein A, Protein A alkaline stable, Protein G, Protein A/G, and Protein L immobilized resins that have specific affinity to immunoglobulins, allowing purification of all antibodies of a particular target class regardless of antigen specificity.
We offer a broad range affinity purification products designed for the purification of recombinant proteins—specifically cobalt and nickel ion-metal affinity chromatography (IMAC) resins for His-tagged proteins and immobilized glutathione resins for GST-tagged proteins. Small-scale resins are also available for c-myc and HA-tagged proteins.
The strong association between avidin and biotin has been successfully employed in various affinity purification schemes. We offer a variety of immobilized biotin binding supports, including avidin, streptavidin, NeutrAvidin protein, Invitrogen CaptAvidin, and monomeric avidin resins.
We offer kits in a range of formats for various immunoprecipitation (IP) and co-immunoprecipitation (co-IP) strategies, as well as products for capturing protein-nucleic acid interactions (ChIP and RIP) and solutions for enriching recombinant or modified proteins (pull-down assays).
Pipette tip-based, ready-to-use Thermo Scientific MSIA microcolumns enable affinity purification of very small quantities of target analytes from complex biological samples. Use robotics-ready MSIA microcolumns to immunoprecipitate and purify cleaner analytes with enhanced reproducibility.
Protein enrichment strategies involve the selective isolation of protein subclasses sharing a common post-translational modification (PTM), such as phosphorylation and glycosylation, typically via affinity ligands such as ion-metal affinity chromatography (IMAC) or immobilized lectins.
We’ve developed activated supports for the immobilization of proteins and other molecules for target-specific affinity purification. The functional groups on the resins utilize a range of coupling mechanism for immobilizing amine-, sulfhydryl-, carbonyl-, and carboxyl-based targets.
For your convenience, we offer a wide range of disposable plastic columns (spin- and gravity-based) for small-scale purification needs, Thermo Scientific BupH Dry-Blend Buffer Packs, and Thermo Scientific Pierce 10X and 20X Concentrated Buffers.
When you need high performance and high throughput on a large scale, POROS bulk chromatography resins are the industry standard for process-scale bioseparations.
Mini- and microcentrifuges to accelerate your protein preparation protocols with RCFs up to 21,000 x g in compact, safe, easy-to-use instruments.
Designed for solid performance and consistent results, our benchtop centrifuges deliver efficient sample processing for your protein purification & isolation applications.
For Research Use Only. Not for use in diagnostic procedures.