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Amplified Fragment Length Polymorphism-AFLP® Analysis
AFLP® is a technique used to detect polymorphisms in DNA when no information about the genome is known. Following restriction enzyme digestion of DNA, a subset of DNA fragments is selected for PCR amplification and visualization. A unique fingerprint is generated for a particular genome. AFLP®, first developed for plant studies, is now used for a wide variety of genetic analysis applications.
Click image to enlargeFigure 1.
Figure 1. Analysis of AFLP® assay data from the P1, P2, and F1 samples. Plots on the left contain the AFLP® peaks (blue) and size standard peaks (red) for the three samples. The plot on the right side shows the bins (gray bars) and allele calls.
The power of AFLP® analysis is the speed with which the technique can quickly generate large numbers of marker fragments for any organism, without the need for any sequence data.
Step-by-Step Guide to AFLP® Analysis
 DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: DNA Isolation |
 Cut genomic DNA into fragments with the restriction enzymes Msel and EcoRI and Ligate adaptors. Recommended Products: Perform Restriction digestion and LigationWhich Products are Right for You? |
 Recommended Products: Perform PCR
|
 Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis. Recommended Products: Prepare Sample for AnalysisSizing InformationTo learn more about our GeneScan Size standards used in sizing experiments please see our table below.
|
 During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the AFLP profile.
Recommended Products: Data Analysis |
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: DNA Isolation |
Cut genomic DNA into fragments with the restriction enzymes Msel and EcoRI and Ligate adaptors. Recommended Products: Perform Restriction digestion and LigationWhich Products are Right for You? |
Recommended Products: Perform PCR
|
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis. Recommended Products: Prepare Sample for AnalysisSizing InformationTo learn more about our GeneScan Size standards used in sizing experiments please see our table below.
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the AFLP profile.
Recommended Products: Data Analysis |
Step-by-Step Guide to AFLP® Analysis
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: DNA Isolation |
Cut genomic DNA into fragments with the restriction enzymes Msel and EcoRI and Ligate adaptors. Recommended Products: Perform Restriction digestion and LigationWhich Products are Right for You? |
Recommended Products: Perform PCR
|
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis. Recommended Products: Prepare Sample for AnalysisSizing InformationTo learn more about our GeneScan Size standards used in sizing experiments please see our table below.
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the AFLP profile.
Recommended Products: Data Analysis |
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: DNA Isolation |
Cut genomic DNA into fragments with the restriction enzymes Msel and EcoRI and Ligate adaptors. Recommended Products: Perform Restriction digestion and LigationWhich Products are Right for You? |
Recommended Products: Perform PCR
|
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis. Recommended Products: Prepare Sample for AnalysisSizing InformationTo learn more about our GeneScan Size standards used in sizing experiments please see our table below.
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the AFLP profile.
Recommended Products: Data Analysis |
Highlighted products for ISSR Analysis:
Visistat reference component
For Research Use Only. Not for use in diagnostic procedures.