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Glycan analysis
Advancing eluents
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Getting started

Whether you are new to ion chromatography for carbohydrate analysis and looking for a guide to get you started, or an experienced user wanting to take your analysis to the next level, you will find the information here to help you make the right choices. 

  See why ion chromatography is a good choice of technique for your carbohydrate analysis as detailed in this white paper › 

Take one of our on-line learning modules to understand the fundamentals of carbohydrate analysis using high performance anion exchange with pulsed amperometric detection (HPAE-PAD) covering the theory and basics of the technique, why eluent preparation is so important and which column is the right choice for your application.   

Learn more about the basics of carbohydrate analysis using high performance anion exchange with pulsed amporemetric detection (HPAE-PAD) in these on-line tutorials.

Determine the right column for you

This handy columns selection guide for carbohydrates analysis will help you determine which is the right column for you.

Learn more about carbohydrates in food testing

If you are involved in food testing, head to our dedicated carbohydrates testing in food page for more detailed information on food applications.

Complex carbohydrate analysis

Complex carbohydrate analysis

If you are looking at more complex carbohydrates such as oligosaccharides or glycans, learn how the latest column technology can help: Filling the gaps in glycan analysis with the Thermo ScientificTM DionexTM CarboPacTM PA300 column.

Here we will find out about the Dionex CarboPac PA300 column and what areas we can resolve to understand glycans better, whether it's O-linked or N-linked, and if it's charged or neutral.

Using eluent generation to advance carbohydrate analysis

Simplify the complexity of carbohydrate analysis with eluent generation

From simple mono- and disaccharides to the more complex oligosaccharides - you often require multiple method conditions to separate all compounds.

This means a range of eluents and buffers may be required. Sourcing the purest chemicals to use for eluents can be challenging. Many cautious steps are needed to manually prepare eluents to ensure good reproducible data. Method development can take longer due to multiple eluent make ups.

Automated eluent generation can simplify carbohydrate analysis by providing:

  • Cleaner and purer eluents
  • Reproducible eluents, day to day, user to user, lab to lab
  • Minimal need for maintenance to your instrument
  • Time and resource savings
  • Smarter working and increased productivity
  • Ability to run gradients on an isocratic pump.

 
 
 
What is eluent generation?

What exactly does eluent generation mean?

What exactly does eluent generation mean?

This whitepaper explains how eluent generation is an electrolytic process allowing the production of high purity eluents for ion chromatography, also known as Reagent Free ion chromatography. The entire process only requires deionized water, eliminating the need to handle any acids or bases.

How does eluent generation work? This whiteboard video shows the chemistry behind eluent generation, the types of eluents that can be generated and the benefits this can bring to your workflows. Understand the role eluent generation plays in ion chromatography.  

 
 
 
What is dual eluent generation?

Dual eluent generation can be used for separations where sodium acetate buffers are usually required.

As well as the benefits of eluent generation detailed above, dual eluent generation also enables you to:

  • 'Just add water' to analyse complex carbohydrates without the need for sodium hydroxide/ sodium acetate gradients
  • Eliminate the risk of contamination from lower quality sodium acetate.

Find out more about dual eluent generation

 
 
 
Further information on complex carbohydrate analysis

Related application notes

Glycoprotein analysis

Hear how our customers at SGS Life Sciences, West Chester, USA are using ion chromatography to profile glycans in biological products.

 
 
 
Looking to advance even further with HPAE-PAD and mass spectrometry?

Webinar: Towards a more complete glycome - Advances in ion chromatography-mass spectrometry (IC-MS) for improved separation and analysis of carbohydrates

This on-demand webinar details how HPAE-PAD can be linked to mass spectrometry to advance carbohydrate analysis to the maximum to access data on the acidic subsets of the glycome to compliment LC-MS based methods.

Learn more about glycan and biopharmaceutical applications ›

Are you an existing user and need some practical support, find out more here

Troubleshooting your Carbohydrate Analysis

Troubleshooting your carbohydrate analysis

In this section we will cover best practices for maintaining your system for optimized output, and how to troubleshoot common carbohydrate analysis problems, such as poor signal-to-noise ratios, pressure increases and poor peak retention.








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