Choose transfection solutions for DNA, RNA, mRNA, and protein delivery to best suit your experiments.  Our products are designed to provide you with high transfection efficiency and cell viability. 

Plasmid transfection

Select plasmid transfection products

Plasmid miniprep, midiprep, maxiprep, megaprep, and gigaprep kits
Pure Link HiPure Plasmid Filter Maxiprep Kit
  • Low endotoxin (0.1–1 EU/µg)
  • Yield up to 20 µg–15 mg
  • Total protocol time of 30–120 min
Lipofectamine 3000 reagent
Lipofectamine 3000 box and tubes
  • Superior efficiency—10-fold higher efficiency into the broadest spectrum of difficult-to-transfect cells
  • Gentle with low toxicity—for improved cell viability
  • Best value—just pennies per reaction for best-in-class transfection results

siRNA or miRNA transfection

Select siRNA or miRNA products

Silencer Select siRNAs
Plates of Silencer Select siRNAs
  • Up to 100-fold more potent than other available siRNAs and fewer off-target effects
  • Reduced off-target effects up to 90% due to locked nucleic acid (LNA) chemical modifications
  • 100% guaranteed to silence, the best guarantee in the industry
mirVana miRNA mimics and inhibitors
mirVana miRNA mimic and inhibitor control
miRNA mimics
  • For microRNA gain-of-function studies
  • Highly specific due to chemical modifications that prevent passenger strand entry into RISC

miRNA inhibitors

  • For microRNA loss-of-function studies
  • Potent, long-lasting microRNA inhibition due to proprietary design and chemical modifications

For in vitro transfection

Lipofectamine RNAiMAX Reagent
Lipofectamine RNAiMAX Reagent box and tubes
  • Provides highly efficient siRNA/miRNA delivery with low cytotoxicity
  • Highly versatile across a wide range of cell types
  • Ideal for high-throughput siRNA/miRNA transfections

For in vivo transfection

Invivofectamine 3.0 Transfection Reagent
Invivofectamine 3.0 Transfection Reagent Starter Pack
  • Impressive results with up to 85% knockdown achieved using microgram levels of siRNA
  • Use up to 90% less siRNA than required with previous reagents for efficient, effective knockdown in vivo

mRNA transfection

MEGAscript transcription kits
MEGAscript T7 Transcription Kit
  • For synthesis of large amounts of unlabeled or low specific activity RNA
  • Yields over 10 times more RNA than conventional in vitro transcription reactions
mMESSAGE mMACHINE transcription kits
mMESSAGE mMACHINE™ SP6 Transcription Kit
  • For synthesis of large amounts of capped RNA
  • Yields up to 10–50X conventional in vitro transcription reactions
Lipofectamine MessengerMAX Transfection Reagent
Lipofectamine MessengerMAX Reagent box and tube
  • Faster protein expression with no risk of genomic integration with mRNA-based transfection
  • Impressive mRNA delivery performance with >60% transfection efficiency in neurons and primary cells

CRISPR-Cas9 transfection

Select DNA, RNA or Protein

GeneArt CRISPR-Cas9 vectors
GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit
  • All-in-one vector system for CRISPR-based genome editing
  • Affordable, ready-to-use cloning vectors
  • Provides enrichment for hard-to-transfect or difficult-to-modify cell lines
Lipofectamine 3000 reagent
Lipofectamine 3000 box and tubes
  • Superior efficiency—10-fold higher efficiency into the broadest spectrum of difficult-to-transfect cells
  • Gentle with low toxicity—for improved cell viability
  • Best value—just pennies per reaction for best-in-class transfection results
TrueGuide synthetic guide RNA (sgRNA)
Illustration of TrueGuide Synthetic gRNA
  • Ready-to-transfect, chemically modified CRISPR sgRNAs
  • Designed by a proprietary algorithm for high-efficiency editing
  • Predesigned for knockout or create a custom design for precise gene editing
GeneArt CRISPR Nuclease mRNA
Tube and box of GeneArt CRISPR Nuclease mRNA
  • Ready to co-transfect with a target-specific guide RNA (gRNA)
  • Smaller payload than plasmid-based Cas9 systems for improved delivery into the cell and better genome editing efficiency
  • Can be used with more than one gRNA to edit multiple genomic loci with one transfection

For transfection of sgRNA only to Cas9-expressing cells

Lipofectamine RNAiMAX Reagent
Lipofectamine RNAiMAX Reagent box and tubes
  • Provides highly efficient sgRNA delivery with low cytotoxicity
  • Highly versatile across a wide range of cell types
  • Ideal for high-throughput sgRNA transfections  

For co-transfection of sgRNA and Cas9 mRNA

Lipofectamine MessengerMAX Transfection Reagent
Lipofectamine MessengerMAX Reagent box and tube
  • Faster protein expression with no risk of genomic integration
  • >60% transfection efficiency in neurons and primary cells
TrueCut HiFi Cas9 Protein
Tube and box of TrueCut Cas9 Protein v2
  • Significantly reduce off-target effects in a broad range of cell types including primary, stem and immune cells
  • Preserved high on-target editing efficiency relative to TrueCut Cas9 v2 (wild type)
Lipofectamine CRISPRMAX Cas9 Transfection Reagent
Lipofectamine CRISPRMAX Cas9 Transfection Reagent
  • Provides highly efficient delivery of RNP (Cas9:gRNA complex) into cells with low toxicity
  • Demonstrated high cleavage efficiency in a broad range of cell types
  • Compatible with high-throughput setup
Quick tip: Designing custom genome editing?

The Invitrogen TrueDesign Genome Editor is a free online tool that enables scientists of all experience levels to easily design, select, and order reagents for accurate and successful gene editing experiments.

Try TrueDesign Genome Editor

Cotransfection of DNA, RNA, protein

Lipofectamine 3000 reagent
Lipofectamine 3000 box and tubes
  • Superior efficiency—10-fold higher efficiency into the broadest spectrum of difficult-to-transfect cells
  • Gentle with low toxicity—for improved cell viability
  • Best value—just pennies per reaction for best-in-class transfection results
Neon Transfection System

Performing electroporation? 

Use the Neon Transfection System for a significant increase in transfection efficiency and cell viability.

Learn more
Invitrogen TransfectionSelect product selection tool
Pro tip

Find recommended transfection solutions, protocols, citations quickly with the TransfectionSelect product selection tool. 

Try now
100 ml bottle of Opti-MEM I Reduced Serum Medium
Quick tip

 

Don’t forget Opti-MEM I Reduced Serum Medium to prepare the transfection complex.

Need more information?

When selecting a transfection method, it is important to consider the payload you wish to deliver (DNA, RNA, or protein) and the type of cells you want to transfect. 

For Research Use Only. Not for use in diagnostic procedures.