two scientists at a table pipetting solutions

Collaborating to accelerate your discovery

Advanced design and viral production services enable you to move forward toward your next discovery. From design, synthesis, and  production of viral particles, we offer end-to-end services to support every step in your lentivirus (LV) and Adeno-associated virus (AAV) RUO production. Our dedicated staff of R&D scientists and project managers are ready to partner with you and your colleagues to help accelerate your research program. If you’re looking for larger-scale production or seeking CDMO guidance on virus production.

Lentivirus and Adeno-Associated Virus RUO production available

  • We offer a complete end-to-end service for the creation of lentiviral and adeno-associated virus expression constructs and virus packaging
  • Packaged and titered virus, ready in as little as 4 weeks
  • Different promoters and selection markers available for maximum flexibility for transfer plasmid construction
     

Overview of RUO virus production process

Process diagram showing steps from plasmid development and virus production to purification, QC, and delivery to customer.

Viral production workflow

  • 100 mL production scale (please inquire for production scale of up to 1L)
  • Uses Gibco LV-MAX or AAV-MAX system CTS-grade for easy scale-up
  • Packaged virus particles can be concentrated up to 100X
  • Titer determined by p24 ELISA for Lentivirus or qPCR for AAV
  • Optional functional titer of viral particles available as a separate assay
  • Final deliverable: concentrated virus particles, 1 mL (5 x 200 µL) with an average titer range between 106–109  for lentivirus and 109–1012 for AAV
Step 1 : Gene synthesis
Step 2 : Virus packaging
  • 100 mL production scale (please inquire for production scale of up to 1L)
  • Uses Gibco LV-MAX or AAV-MAX system CTS-grade for easy scale-up
  • Packaged virus particles can be concentrated up to 100X
Step 3 : Titer
  • Titer determined by p24 ELISA for Lentivirus or qPCR for AAV
  • Optional functional titer of viral particles available as a separate assay
  • Final deliverable: concentrated virus particles, 1 mL (5 x 200 µL) with an average titer range between 106–109  for lentivirus and 109–1012 for AAV

Viral production depends on advanced technologies

The Gibco Lentiviral Production systems are the first optimized systems that provide all the high-quality components you need in a chemically defined, serum-free environment.

Gibco AAV Max Helper-Free AAV Production System Kit

AAV-MAX is a scalable, high-titer platform for suspension AAV production that can help enable you to seamlessly transition from discovery to commercial production.

  • High AAV titers
  • Scalability
  • Animal origin–free (AOF)
  • Clonal 293F-derived producer cells

Learn more

bottles and vials that make up the AAV-MAX Helper-Free AAV Production System Kit

Gibco LV-MAX Lentiviral Production System

LV-MAX is designed to seamlessly transition from discovery to commercial production, offering a scalable and high-yield lentiviral production platform. It is a suspension based, high-density culture of HEK 293–derived Viral Production Cells adapted to a special chemically defined, serum-free and protein-free LV-MAX Production Medium.

  • High titers
  • Scalability
  • Animal origin–free (AOF)

Learn more

bottle and vials for the LV-MAX Lentiviral Production System
Featured video
Thermo Fisher Cell Biology Services: Virus Production

A short video describing our service capabilities around virus production.

Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.