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NGS Sequencing Support
Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
- For my application, what kits do I need for sequencing on the Ion Torrent™ Ion PGM™ system?
- What Ion PGM chips are available? How much sequencing data can I expect from each chip type?
- How many sequencing runs can be performed from a single kit?
- How do I update my Ion PGM to the latest software version?
- How many samples can I multiplex on a single chip for sequencing on the Ion PGM or Ion Torrent™ Ion Proton™ System?
Troubleshooting
- What should I do if R1, R2, R3, and/or R4 fails at Raw Traces and the screen turns red during the last step of initialization of my Ion PGM system?
- What should I do if W1 fails at Raw Traces and the screen turns red during the last step of initialization of my Ion PGM System?
- What should I do if a “No Connect FTP Server” alert appears on the Ion PGM touch screen?
- What should I do if the message “There may be a blockage or no NaOH in W1. Please check W1 and run line clear then try again” appears during initialization of my Ion PGM System?
- If the Chip Check fails on my Ion™ S5/Ion S5™ XL Instrument, what should I do?
Guides and Tools
General
- Application Note: The Ion PGM™ System, With 400-base Read Length Chemistry, Enables Routine High-Quality&de novoAssembly of Small Genomes
- Application Note: Discover Variants in 46 Cancer Genes Using the Ion AmpliSeq™ Cancer Panel v1
- Application Note: Ion AmpliSeq™ Designer Provides Full Flexibility to Sequence Genes of your Choice
- Application Note: Rapid Sequencing of Microorganisms with the Versatile Ion S5™ Next-Generation Sequencing Systems
- Application Note: Enabling Reproductive Genomics and Inherited Disease Research with the Ion S5™ Next-Generation Sequencing System
- Brochure: Ion S5™ and Ion S5™ XL Systems
- Brochure: Ion AmpliSeq™ DNA Custom Panels
- Brochure: Ion AmpliSeq™ Comprehensive Cancer Panel
- Brochure: Ion AmpliSeq™ Inherited Disease Panel
- Brochure: Ion AmpliSeq™ Sample ID Panel
- Manual: Ion S5™ and Ion S5™ XL Instrument
- Schematic: A History of Innovation in Genetic Analysis
Template Preparation and Sequencing Protocols
Ion Chef System with Ion S5 System/Ion S5 XL System
- Quick Reference: Ion 510 & Ion 520 & Ion 530 Kit - Chef
- Quick Reference: Ion 540 Kit - Chef
- Quick Reference: Ion 550 Kit - Chef
- User Guide: Ion 510 & Ion 520 & Ion 530 Kit - Chef
- User Guide: Ion 540 Kit - Chef
- User Guide: Ion 550 Kit - Chef
Ion Chef System with Ion PGM System
- Quick Reference: Ion PGM Hi-Q View Chef Kits
- User Guide: Ion PGM Hi-Q View Chef Kits
Ion Chef System with Ion Proton System
- Quick Reference: Ion PI Hi-Q Chef Kit
- User Guide: Ion PI Hi-Q Chef Kit
Ion OneTouch 2 System with Ion S5 System/Ion S5 XL System
- Quick Reference: Ion 520 and Ion 530 Kit for the Ion OneTouch 2 System
- Quick Reference: Ion 540 Kit for the Ion OneTouch 2 System
- User Guide: Ion 520 and Ion 530 Kit for the Ion OneTouch 2 System
- User Guide: Ion 540 Kit for the Ion OneTouch 2 System
Ion OneTouch 2 System with Ion Proton System
- Quick Reference: Ion PI Hi-Q Sequencing 200 Kit
- User Bulletin: Demonstrated Protocol: Initialization for 2×520 Flow Runs with the Ion PI Sequencing 200 Kit v3
- User Guide: Ion PI Hi-Q Sequencing 200 Kit
- User Guide: Ion PI Sequencing 200 Kit v3
- User Guide: Ion PI Sequencing 200 Kit v2
Ion OneTouch 2 System with Ion PGM System
- Quick Reference: Ion PGM Hi-Q View Sequencing Kit
- Quick Reference: Ion PGM Hi-Q Sequencing Kit
- Quick Reference: Ion PGM Sequencing 200 Kit v2
- User Guide: Ion PGM Hi-Q View Sequencing Kit
- User Guide: Ion PGM Hi‑Q Sequencing Kit
- User Guide: Ion PGM Sequencing 200 Kit v2
Search our database for Product Documents: Manuals, Vector Maps, Citations & References
Search our database for Quality and Safety Documents: MSDS, COA
- Overview: Next-Generation Sequencing
- Overview: Ion Torrent Next-Generation Sequencing Applications
- Overview: Ion Torrent Next-Generation Sequencing Workflow
- Overview: Ion Torrent Next-Generation Sequencing Technology
- Overview: Sequencing Education
- Overview: Targeted Sequencing by Ion Torrent Next-Generation Sequencing
- Quote Request: Ion AmpliSeq Request a Quote
- Citations: Ion Torrent Next-Generation Sequencing Publications & Literature
- Selection: Choose Next-Generation Sequencing or Sanger Sequencing Solutions
- Video: Introduction to Ion Torrent Next-Generation Sequencing Technology
- Videos: Ion Torrent Next-Generation Sequencing Featured Researchers
- Videos: Ion Torrent Videos
- Video: Ion S5™ Next-Generation Sequencing System Workflow
- Videos: Ion S5™ and Ion S5™ XL Next-Generation Sequencing Systems Customer Videos
- Workflow: Ion Torrent Next-Generation Sequencing Workflow
- Ion Academy: An exclusiveIon-authorized user educational group with live webinar session featuring the latest Ion Torrent information
- Browse our site for: Training, Events, and Webinars
- Ion AmpliSeq Designer: The Ion AmpliSeq Designer allows you to design your own AmpliSeq panel using the preloaded genomes or by uploading your own reference sequence.
- Instrument Service Coverage Lookup - Find warranty and service contract information for your instrument
Browse our site for: Mobile and Desktop Apps
Custom Services
Ion Torrent Certified Service Provider Program
Browse our global network of validated service providers that helps enable researchers in small and medium-sized laboratories to get high-quality exome and transcriptome sequencing data generated on the Ion Proton System from small amounts of input material with very fast turnaround. We offer services including whole-exome sequencing, whole-transcriptome sequencing, and targeted transcriptome sequencing.
Ion AmpliSeq Custom DNA Panels
We can create and design customized Ion AmpliSeq Panels to fit your needs. Use our free online assay design tool that connects you directly to our assay design pipeline. Panels can be designed against a number of preloaded genomes, or for any other organism by uploading your own FASTA file reference sequence.
Ion AmpliSeq Community Panels
Leveraging the power of the Ion Community and Ion AmpliSeq technology, we have developed Ion AmpliSeq Community Panels. Designed with leading disease researchers, Ion AmpliSeq Community Panels provide the ability to selectively analyze gene content with the aim of transforming cancer and inherited disease research. Your disease research is greatly simplified with the community-wide availability of these panels. In addition, for maximum flexibility, you will be able to rapidly customize any Ion AmpliSeq Community Panel to advance clinical research to fit your own project requirements.
For Research Use Only. Not for use in diagnostic procedures.