The QuantStudio 3D Digital PCR System has been discontinued since March 2023 and we recommend the QuantStudio Absolute Q Digital PCR System. Access to the AnalysisSuite app will be available until March 31, 2025. Customers are encouraged to download their data.

Having difficulties with your experiment?

We are dedicated to your success. Get back on track. View our expert recommendations for commonly encountered problem scenarios.

View the relevant questions below:

Beginning your experiment? Visit our

Getting Started page

Instrument

Is there a user group where I can find more help?

For troubleshooting guides, please register for our QuantStudio™ 3D Digital PCR System User Group Forum in the Applied Biosystems community.

How do I replace the fuses in the QuantStudio™ 3D Digital PCR System?

Please follow the procedures described in "Chapter A: Maintenance" of the QuantStudio™ 3D Digital PCR System User Guide to replace the fuses. Make sure to only replace with those listed and certified fuses of the same type and rating as those currently in the QuantStudio™ 3D instrument.

How do I update the firmware on the QuantStudio™ 3D Digital PCR System?

Please follow the procedures starting in "Chapter A: Maintenance" of the QuantStudio™ 3D Digital PCR System User Guide to update the instrument firmware.

I’m having trouble using AnalysisSuite™ software on Internet Explorer. What is wrong?

AnalysisSuite™ software is optimized for Google Chrome v4.0 or later. Firefox also works, but Internet Explorer is not recommended.

Data Analysis

How do I convert copies/µL to ng/µL?

First you need to know the amount of nanograms per copy of your sample. In this example, we’ll use human genomic DNA as the source of DNA.

dPCR-5

Now you just need to multiply ng/copy by copies/µL. You have 2,500 copies/µL of human gDNA.

     2,500 copies/µL x 0.0033 ng/copy = 8.25 ng/µL

How do I figure out the concentration of my DNA stock from the results I get from the QuantStudio™ 3D Digital PCR System?

If you include all of the necessary dilution factors into the AnalysisSuite™ software, the software will give you the copies/µL in your stock. There are 2 dilutions you need to take into account. The first is the dilution of the sample in the reaction. The second is the dilution of the stock that you make before adding it to the digital PCR reaction.

For example, say you want to add 1 µL of a sample that has been diluted 1:10 from the stock. If you add 1 µL of your sample to a 16 µL (final volume) reaction, the dilution factor of the sample is 1:16 or 1/16 = 0.0625. Since the stock has also been diluted 1:10 (0.1), you also need to factor this in. The final dilution factor to enter into the software is 0.0625 x 0.1 = 0.00625 (1:160). As you can see below, you can use either annotation to indicate the dilution factor in the AnalysisSuite™ software. If you enter that value into the “Dilution” column, the software will give you the copies/µL in your starting material (stock).

dPCR-4

Need more information? Contact us ›

For Research Use Only. Not for use in diagnostic procedures.