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Raw Materials for Molecular Diagnostics
Thermo Fisher Scientific has more than 45 years of experience developing enzymes, buffers, nucleotides, ladders, nucleic acids, and other reagents for life science research. Our dedicated OEM teams partner with customers with product development, manufacturing, and commercialization.
In your molecular assay development journey, our experts help you to select the best-fitting products for your application. Our R&D scientists support assay development efforts in RT-PCR, isothermal amplification, and NGS methods.
Raw materials for custom assay development
Molecular diagnostic assays and kits require high quality reagents. Our vast portfolio of reagents is available off the shelf in bulk quantities or can be customized for your specifications. Figure 1 lists our most popular customization capabilities.
Figure 1. Overview of customization capabilities.
Large-scale manufacturing and production
We offer large scale manufacturing and production services include sourcing and quality control of raw materials, manufacturing the assay components, assembling kits, packaging, customizing labels, and more.
Custom reagents and material modification
We tailor our existing products to your specifications. This may involve modifying existing assays to fit specific sample types, concentration and composition, or stability requirements.
Custom assay design and development
We offer comprehensive expertise in developing and designing assays, including but not limited to protocol design, reagent and components selection, condition optimization, performance evaluation, quality validation, and regulatory compliance. These services are available for protein cloning, expression, and purification, enzyme evolution, and modification of RT and PCR enzymes and buffers.
Reagents for custom assay development
Custom assay development relies on quality reagents optimized for molecular diagnostic assay. Reagents and enzymes commonly used in custom assay development are listed in Tables 1 to 5. Click on the accordions to see the tables.
Table 1. Reverse transcriptases available for assay development.
| Enzymes | Applications | ||||
|---|---|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR | One-step RT-PCR | LAMP | cDNA synthesis | |
| Invitrogen Lyo-ready SuperScript Reverse Transcriptase, One-step RT-qPCR | ✓ | – | – | – | – |
| Invitrogen SuperScript IV Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Invitrogen SuperScript III Reverse Transcriptase | – | – | ✓ | – | – |
| Thermo Scientific Maxima Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific Maxima H Minus Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific RevertAid Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific RevertAid H Minus Reverse Transcriptase | – | ✓ | ✓ | ✓ | ✓ |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction LAMP: Loop-mediated isothermal amplification | |||||
View the enzyme selection guide to select products suitable for your application
Table 2. DNA polymerases available for assay development.
| Enzymes | Applications | ||
|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR | PCR | |
| Invitrogen Platinum II Taq Hot-Start DNA polymerase | ✓ | ✓ | ✓ |
| Invitrogen Platinum Taq DNA Polymerase | ✓ | ✓ | ✓ |
| AmpliTaq Gold DNA Polymerase | ✓ | ✓ | ✓ |
| AmpliTaq Gold 360 DNA Polymerase | ✓ | ✓ | ✓ |
| Thermo Scientific LibertyTaq DNA Polymerase | ✓ | ✓ | ✓ |
| Thermo Scientific Taq DNA Polymerase, recombinant | ✓ | ✓ | ✓ |
| Invitrogen Platinum SuperFi II DNA Polymerase | – | – | ✓ |
| Invitrogen Platinum SuperFi DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion Plus DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion Hot Start II DNA Polymerase | – | – | ✓ |
| Thermo Scientific Phusion U Hot Start DNA Polymerase | – | – | ✓ |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction | |||
View the enzyme selection guide to select products suitable for your application
Table 3. Modifying enzymes available for assay development.
View the enzyme selection guide to select products suitable for your application
Table 4. dNTPs available for assay development.
| Products | Applications | ||||
|---|---|---|---|---|---|
| One-step RT-qPCR | Two-step RT-qPCR | LAMP | cDNA synthesis | ||
| Thermo Scientific dNTP Mix |
| ✓ | ✓ | ✓ | ✓ |
| Thermo Scientific dATP Solution (100mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dCTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dGTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dTTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| Thermo Scientific dUTP Solution (100 mM) | ✓ | ✓ | ✓ | ✓ | |
| RT-qPCR: Reverse transcription quantitative polymerase chain reaction LAMP: Loop-mediated isothermal amplification | |||||
View the enzyme selection guide to select products suitable for your application
Table 5. Enzymes for isothermal nucleic acid amplification (INAAT)
| Product | Application |
|---|---|
| Invitrogen Lyo-ready Bst DNA Polymerase | LAMP, HDA, EXPAR, RCA |
| Invitrogen SuperScript IV RT-LAMP Master Mix | LAMP |
| Invitrogen SuperScript IV Reverse Transcriptase and other RTs | LAMP, NASBA, TMA |
| Thermo Scientific T7 RNA Polymerase | NASBA, TMA |
| RNase inhibitors (Ribolock, RNaseOUt, and others) | LAMP, NASBA, TMA |
| RNase H | NASBA |
| Thermo Scientific Phi29 DNA Polymerase | MDA, WGA, RCA |
| Thermo Scientific EquiPhi29 DNA Polymerase | MDA, WGA, RCA |
| Thermo Scientific Klenow Fragment, exo– | SDA |
| EXPAR: Exponential amplification reaction HDA: Helicase-dependent amplification LAMP: Loop-mediated isothermal amplification MDA: Multiple displacement amplification NASBA: Nucleic acid sequence-based amplification RCA: Rolling circle amplification SDA: Strand displacement amplification TMA: Transcription-mediated amplification WGA: Whole-genome amplification | |
Buffers & other reagents
- DNA Ladders
- RNA Ladders
- Water
- BSA
- DTT
- PolyA
- Reaction buffers
- DMSO
- Co-Enzyme A
View the enzyme selection guide to select products suitable for your application
Resources for custom assay development
Explore our portfolio of products compatible for custom assay development
FAQs: Raw materials for custom assay development
No, High Fidelity enzymes are not compatible with TaqMan probes qPCR. Proofreading DNA polymerases lack 5’→ 3’-exonuclease activity which is essential for TaqMan assay.
Platinum II Taq Hot-Start DNA Polymerase works best with inhibitor-containing samples.
We recommend using Lyo-ready SuperScript Reverse Transcriptase 1-step RT-qPCR, which was specially created for one-step RT-qPCR application. We have prepared an optimized protocol for a one-step RT-qPCR application that contains Lyo-ready SuperScript Reverse Transcriptase, 1-step RT-qPCR, and Lyo-ready Platinum II Taq Hot-Start DNA Polymerase.
Read application note: SARS-CoV-2 RNA detection with the lyo-ready one-step RT-qPCR system
View the user guide: Lyo-ready one-step RT-qPCR system
We do not recommend using SuperScript IV reverse transcriptase for one-step RT-qPCR assay due to the high affinity to the template. This feature for one-step RT-qPCR application is excessive because it might prevent polymerase to act efficiently. SuperScript IV Reverse Transcriptase is the number one enzyme of choice for the NGS library preparation step as well as the two-step RT-qPCR assay.
Yes, Collibri Cleanup Beads and AMPure XP are interchangeable but note the differences in the cleanup/size selection protocols.
Yes, all Thermo Fisher Scientific DNA/RNA modifying enzymes are rigorously tested and suitable for NGS library prep workflows.
Resources
Check out our resources on molecular assay development.
Technical literature
Access technical and application notes on molecular cloning.
Webinars
Stay up-to-date on industry trends in molecular diagnostics with on-demand webinars from industry experts.
Digital handbooks
Review content on workflow, troubleshooting, strategies, and selection guides.