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The Applied Biosystems TrueMark MSI Assay is a low-input, single-amplification solution for the rapid identification of microsatellite instability (MSI) in formalin-fixed paraffin-embedded (FFPE) samples from multiple tumor tissue types for research applications. The included analysis and reporting software featuring automated calling can help you have a more efficient workflow.
Microsatellite instability (MSI) is the condition of genetic hypermutability that results from impaired DNA mismatch repair (MMR). Studies indicate that the presence of high levels of MSI (MSI-H) may potentially serve as a marker for some conditions associated with increased cancer spread across a variety of tumor types, including colorectal, gastric, and endometrial cancers.
Defects in MMR also result in novel somatic mutations in unrelated loci throughout the genome. These mutations can produce immunogenic proteins that can “prime” an immune response to the foreign neoantigens in the tumor. MSI-H tumors are potentially more sensitive to immune checkpoint inhibitors than microsatellite-stable (MSS) tumors. There is therefore substantial interest in studying MSI in diverse tumors to identify those in which growth might be slowed by immune checkpoint inhibitors.
The TrueMark MSI Assay is a low-input, affordable assay with an expanded 13-MSI-marker panel and includes TrueMark MSI Analysis Software. The entire workflow takes as little as four hours to determine sample status. The TrueMark MSI Assay leverages a 6-dye set to incorporate the additional markers and does not require the use of a tumor-normal match, cutting the number of wells required per sample in half and reducing sample handling burden.
In this recorded webinar, our speakers discuss how genotypic biomarkers are being used to better understand cancer biology, develop prognostic markers, and predict therapeutic success.
This application note describes the development of our new MSI analysis solution that includes an expanded 13-MSI-marker panel and two highly variable STR sequences that can be used to track sample identity. It has been optimized for use on the SeqStudio and 3500 Series genetic analyzers. In addition, we developed software for analyzing the assay that does not require side-by-side analysis of normal, non-tumor tissue. Learn more about the TrueMark MSI Assay by downloading our application note.
In this tech note, we compare three analysis methods (IHC staining for DNA mismatch repair (MMR) proteins, the most commonly used commercial MSI by PCR assay, and the TrueMark MSI Assay) across 162 samples. We show that the TrueMark MSI Assay provides data concordant with IHC and demonstrates increased analytical sensitivity and specificity relative to the alternative commercially available MSI assay. See the comparison data by downloading our tech note.
Included at no additional cost, TrueMark MSI Analysis Software is designed specifically for the TrueMark MSI Assay to enable automated calling of markers and samples, offering greater ease of analysis by reducing the manual QC, analysis, and review required to determine sample status.
Learn more and download the TrueMark MSI Analysis Software ›
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.